Tissue culture method of Pitaya

A technology of tissue culture and dragon fruit, applied in the field of bioengineering, can solve the problems such as affecting the growth of the mother plant, unfavorable expansion of production, slow reproduction speed, etc., and achieves the effects of ensuring genetic stability, easy material sampling, and easy acquisition.

Inactive Publication Date: 2010-11-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the breeding of dragon fruit only relies on cuttings to raise seedlings, the propagation speed is slow and affects the growth of the mother plant, which is not conducive to expanding production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0020] 1. Selection and sterilization of explants: select the mature stems on the vigorous female stems of dragon fruit, first clean the outside of the stems with sterile water, then cut them into triangles with a length of about 2 cm, with at least one axillary bud growth point, Treat with 70% alcohol for about 30 seconds on the aseptic operating table, then disinfect with 0.1% mercuric chloride for 15-18 minutes, rinse with sterile water for 5-7 times for later use;

[0021] 2. Induction of axillary buds: Inoculate axillary bud growth point upwards in MS+paclobutrazol 1.5mg / L medium, inoculate 2-3 explants in each bottle in the inoculation bottle, cultivate under 300lux light, keep the temperature at 24+2°C, and cultivate After about 30 days, the axillary buds will reach a height of 1.5-2.5 cm, cut off and transferred to the proliferation medium;

[0022] 3. Cluster bud induction and proliferation: Cut the excised axillary buds into 2-3 sections and inoculate them in MS+6-BA...

Embodiment 3

[0025] 1. Selection and sterilization of explants: select the mature stems on the vigorous female stems of dragon fruit, first clean the outside of the stems with sterile water, then cut them into triangles with a length of about 2 cm, with at least one axillary bud growth point, Treat with 70% alcohol for about 30 seconds on the aseptic operating table, then disinfect with 0.1% mercury chloride for 15-18 minutes, rinse with sterile water for 5-7 times and set aside;

[0026] 2. Induction of axillary buds: Inoculate the axillary bud growth point upwards in MS+paclobutrazol 0.1mg / L medium, inoculate 2-3 explants in each bottle in the inoculation bottle, cultivate under 300lux light, keep the temperature at 24+2°C, and cultivate After about 40 days, the axillary buds will reach a height of 1.5-2 cm, cut off and transfer to the proliferation medium;

[0027] 3. Cluster bud induction and proliferation: Cut the excised axillary buds into 2-3 segments and inoculate them in MS+6-BA1....

Embodiment 4

[0030] 1. Selection and sterilization of explants: select the mature stems on the vigorous female stems of dragon fruit, first clean the outside of the stems with sterile water, then cut them into triangles with a length of about 2 cm, with at least one axillary bud growth point, Treat with 70% alcohol for about 30 seconds on the aseptic operating table, then disinfect with 0.1% mercuric chloride for 15-18 minutes, rinse with sterile water for 5-7 times for later use;

[0031]2. Induction of axillary buds: Inoculate the axillary bud growth point upwards in MS+paclobutrazol 1.0mg / L medium, inoculate 2-3 explants in each bottle in the inoculation bottle, cultivate under 300lux light, keep the temperature at 24+2°C, and cultivate After about 30 days, the axillary buds reach a height of 2-3 cm, cut off and transfer to the proliferation medium;

[0032] 3. Cluster bud induction and proliferation: Cut the excised axillary buds into 2 to 3 sections and inoculate them in MS+6-BA 2.0mg...

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Abstract

The invention discloses a tissue culture method of pitayas, belonging to the technical field of plant tissue culture. The method comprises the following steps: sterilizing a ripe stem section of a pitaya, inoculating the sterilized stem section on an axillary bud induction culture medium containing MS+0.1-1.5mg/l of paclobutrazol, cutting generated axillary bud and placing on the other culture medium containing MS+6-1.0-6.0mg/l of BA+0.1-1.5mg/l of chlormequat chloride, taking root simultaneously for 42% of test tube seedling which can be transplanted directly with above 95% of transplant survival rate. The method in the invention can be applied to industrialization production, and has the good effect of formula and fast budding. The pitaya germchit produced by the method has the advantages of high genetic stability, strong operability and high application value. By using the method, fast factorial growing seedlings can be realized and pitaya is developed and utilized more effectively.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to plant tissue culture technology, in particular to a method for tissue culture of dragon fruit. Background technique [0002] Dragon fruit (Hylocereus undatus) is a fruit cultivar of the genus Cactaceae (Cactaceae), which originated in Central America and is naturally distributed in Costa Rica, Nicaragua, Mexico, Cuba, etc. Tropical rainforests and deserts in countries and regions. In Asia, dragon fruit is mainly distributed in Taiwan and Vietnam. The fruit of dragon fruit is oval, and the pericarp has two kinds of red and yellow, and the color is bright, and the color of the flesh is red and white. Dragon fruit has high fiber, low calorie, rich in vitamin A, vitamin B, vitamin C, vitamin E, vitamin K, amino acids and mineral nutrition. The soluble solid content of normal fruit is 12% to 13%, the highest reaches 22%, and the content of various vitamins is 46mg / 100g. The sesame-like ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 易自力陈智勇蒋建雄黄丽芳覃静萍艾辛王红权
Owner HUNAN AGRICULTURAL UNIV
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