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Rapid propagating and seedling culturing method by virtue of Bletilla tubers

A Bletilla striata and fast technology, applied in the field of medicinal plant seedling raising engineering, can solve the problems of low cultivation environment, short cultivation time, many operation steps, etc., and achieve the effects of high transplanting survival rate, high application value and high proliferation rate.

Inactive Publication Date: 2014-06-04
ZHANGJIAGANG BENCAO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Due to the low reproduction coefficient of Bletilla striata through pseudobulbs, the operation steps are many, the process is complicated, and the aseptic operation environment and related operating technicians are required in the tissue culture technology, resulting in large investment and low output in group culture. , therefore the purpose of the present invention is to provide a kind of Bletilla striata rapid propagation method that cultivation environment is not high, input is low, and reproduction coefficient is high, reach suitability for suitability for industrialized production, prescription effect is good, germination rate is high, cultivation time is short, plant growth is vigorous, can Strong operability and high application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Put the silt soil on the lowest layer, the silt layer height is 2-6cm, put the nutrient soil on the silt upper layer, the nutrient soil layer height is 5-8cm, spray 0.8% carbendazim solution of 20kg per mu for sterilization; Select the buds on the pseudobulbs of Bletilla striata as the culture material, remove the roots of Bletilla striata, and then separate the small tubers of Bletilla striata, wash them with detergent and tap water, rinse them under running water for 10-30 minutes, and place them in 75% alcohol. Treat it for 10-30 seconds, cut off the discolored part of the explant and set it aside; scratch the small tuber of Bletilla striata on one side with a common scalpel two to three times to a depth of 2-3.5mm, and place it in a pot containing 0.6% gibberella In the seedling induction solution of 0.6% carbendazim and 1.5% 6-benzylaminoadenine, they were treated for 9 minutes, and then the small tubers of Bletilla striata were taken out from the induction solution...

Embodiment 2

[0034] The silt soil is placed on the lowest layer, the silt layer height is 2-6cm, the nutrient soil is put into the silt upper layer, the nutrient soil layer height is 5-8cm, and the 0.6% carbendazim aqueous solution of 30kg is sprayed per mu for sterilization; Select the buds on the pseudobulbs of Bletilla striata as the culture material, remove the roots of Bletilla striata, and then separate the small tubers of Bletilla striata, wash them with detergent and tap water, rinse them under running water for 10-30 minutes, and place them in 75% alcohol. Treat it for 10-30 seconds, cut off the discolored part of the explant and set it aside; scratch the small tuber of Bletilla striata on one side with a common scalpel two to three times to a depth of 1-2.5mm, and place it in a pot containing 0.8% gibberella 0.9% carbendazim and 2.0% 6-benzylaminoadenine in the seedling induction solution for 7 minutes, then take out the small tubers of Bletilla striata from the induction solution...

Embodiment 3

[0036] The silt soil is placed on the lowest layer, the silt layer height is 2-6cm, the nutrient soil is put into the silt upper layer, the nutrient soil layer height is 5-8cm, and the 0.5% carbendazim solution of 35kg per mu is sprayed for sterilization; Select the buds on the pseudobulbs of Bletilla striata as the culture material, remove the roots of Bletilla striata, and then separate the small tubers of Bletilla striata, wash them with detergent and tap water, rinse them under running water for 10-30 minutes, and place them in 75% alcohol. Treat it for 10-30 seconds, cut off the discolored part of the explant and set it aside; scratch the small tuber of Bletilla striata on one side with a common scalpel two to three times, and the depth reaches 1-2.0mm, and put it into a pot containing 0.8% gibberella 0.5% carbendazim, 2.5% 6-benzylaminoadenine seedling induction solution for 7 minutes, then take out the small tubers of Bletilla striata from the induction solution and plac...

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PUM

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Abstract

The invention provides a rapid propagating method of Bletilla. Bletilla seedlings are obtained through the steps of treatment of seedling culturing beds, selection and pretreatment of explants, cluster bud induction, Bletilla seedling root system culturing, Bletilla division culturing, final-period management and the like. The method is very suitable for industrial production, and has the characteristics of excellent formula effect, high bud ratio, short culture time, vigorous plant growth, high transplanting survival rate, strong operability, high application value, low investment, high yield and the like.

Description

technical field [0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a breeding and seedling breeding method for rapidly inducing seedlings by making full use of epidermal tissue on bletilla striata tubers. Background technique [0002] Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption exceeds the regeneration capacity of natural resources, it will inevitably lead to endangered or even extinction of species. Simultaneously along with the day by day destruction of natural ecological environment, also further lead to the scarcity of medicinal plant resources. [0003] Bletilla striata, also known as Baiji and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 向华唐克华
Owner ZHANGJIAGANG BENCAO SCI & TECH
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