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Method for isolated culturing hypericum regenerated plant

A technology for regenerating plants and hypericum, which is applied in botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems that no one has reported on the regeneration of cut hypericum, achieve genetic stability, simple operation, stable genetic effects

Inactive Publication Date: 2009-11-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, no research has been reported on the regeneration of the ornamental cut-off Hypericum

Method used

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  • Method for isolated culturing hypericum regenerated plant
  • Method for isolated culturing hypericum regenerated plant
  • Method for isolated culturing hypericum regenerated plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The ornamental cut flower (cut branch and fruit) hypericum that is publicly sold on the market, the variety is 'Hypericum'Magical Red' (Hypericum'Magical Red') series three varieties of Hypericum are 'Charm Red'3 (code 'Hyp3'), 'Glamour Red' No. 4 ('Hyp4') and 'Glamour Red' No. 7 ('Hyp7'). The stem section of the explant Hypericum supericum was sterilized according to a conventional sterilization method and then inoculated on the MS basic medium with 7.5g / L agar and 3g / L sucrose (referring to: compiled by Li Mingjun, plant tissue culture, China Agricultural Press, 1992, the same below) to carry out subculture;

[0032] (2) Preparation of regeneration medium: MS basic medium + thiadiazophenylurea (TDZ, the concentration is set to 0.05, 0.1, 0.5 mg / L in turn) + α-naphthaleneacetic acid (NAA, the concentration is set to 0.01 in turn, 0.05, 0.1mg / L)+filter paper (Shuangquan brand 9cm quantitative filter paper, one piece of the above-mentioned filter paper is spread on ...

Embodiment 2

[0039] (1) With the aseptic seedling of Hypericum 'Hyp3' kind (the culture step of aseptic seedling sees embodiment 1) as test material, inoculate in the MS basic medium that adds 7.5g / L agar and 3g / L sucrose carry out subculture;

[0040] (2) Preparation of regeneration medium: MS basic medium, supplemented with 7.5g / L agar, 3g / L sucrose, thiadiazophenylurea (concentrations are set to 0.08, 0.10, 0.30, 0.50, 0.80mg / L) , NAA (concentration is set to 0.01mg / L)+1 piece of filter paper (see Example 1 for placement method); adjust the pH of the culture medium to 5.8-6.0, and sterilize at 121 ℃ for 20min by high pressure steam (complete in a clean bench TDZ and filter paper), set aside.

[0041] (3) In the ultra-clean workbench, the 40-45 day hypericum aseptic seedlings that have been subcultured in step (1) are used as test materials, and 4 young tender leaves on the top are cut as explants (after excision of the leaf ends, the its adaxial surface facing the culture medium) was ...

Embodiment 3

[0045] (1) With the aseptic seedling of Hypericum 'Hyp3' variety (see the aseptic seedling (line) part of Example 1 for the source) as the test material, it is inoculated on MS basic with 7.5g / L agar and 3g / L sucrose Subculture in culture medium.

[0046] (2) Preparation of regeneration medium: MS basic medium, supplemented with 7.5g / L agar and 3g / L sucrose, thiadiazophenylurea (concentrations are set to 0.08, 0.10, 0.30, 0.50, 0.80) mg / L+ , NAA (concentration set to 0.01) mg / L + 1 piece of filter paper (see Example 1 for usage); adjust the pH of the medium to 5.8-6.0, and autoclave at 121° C. for 20 minutes. (Complete the addition of TDZ and filter paper in the ultra-clean bench), set aside.

[0047] (3) Under the aseptic condition of the ultra-clean workbench, take subcultured 40-45 days hypericum aseptic seedlings as test material, and cut the top 4 young tender leaves as explants (after excision of the leaf ends, its adaxial surface facing the regeneration medium) was in...

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Abstract

The invention belongs to the plant tissue culturing technical field, discloses a method for isolated culturing hypericum regenerated plant, the steps comprising: A: inoculating non-bacterial seeds of pre-cultivating hypericum different varieties in an MS minimal medium to cultivate for generation; B: formulating regeneration culture medium: MS minimal medium+TDZ0.08-0.8 mg / L+Alpha-fruitone 0.01-0.05 mg / L+filter parer as filter parer bridge; C: under sterilized condition, inoculating the blade of hypericum non-bacterial seeds of hypericum cultured for 40-45 days successively as explants in the MS regeneration culture medium, enabling the paraxial surface to be toward the regeneration culture medium, converting to cultivate under light irradiation after dark cultivating 15-25 days, after 24-26 days, obtaining the regeneration bud; D: inoculating the cutting regeneration bud of the step C in the MS minimal medium to cultivate successively to obtain the complete regenerated plant. The invention is simple, the operation is convenient, the regeneration frequency is high, the preparations of genetic transforming of the cutting flower hypericum and the molecule breeding materials can be quickened.

Description

technical field [0001] The invention belongs to the technical field of industrialized rapid seedling cultivation and molecular breeding of plant tissue culture, and more specifically relates to a method for cultivating hypericum regenerated plants in vitro. Background technique [0002] Hypericum is Hypericum (Hypericum L.), a plant of Garciniaceae (Clusiaceae), with colorful flowers and colorful berries. [0003] In recent years, in the flower market at home and abroad, cut hypericum is very popular among new cut flowers, and it is mainly used as a material in flower arrangement. The fruit-watching Hypericum in the market is mainly H.hircinum; H.frondosum, H.androsaemum and H.inodorum and other interspecific or intraspecific hybrids, such as the 'Charm Red' series (Hypericum 'Magical Red'); Tianzi Powder series (H.'Pinky Flair'), Tianzi Sweetheart series (H.'Sugar Flair'), etc. At present, in the cultivation process, especially many varieties planted in tropical regions s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王彩云张晓慧桂大萍王海菲刘峰黄丽莉
Owner HUAZHONG AGRI UNIV
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