Method for rapidly breeding seedlings by using bletilla striata tubers

Abstract

The invention provides a method for rapidly breeding seedlings by using bletilla striata tubers. According to the method, rapid breeding is realized by using bletilla striata tubers, and the technical route of synchronization of bud increase and rooting is adopted, complete plants can be formed after about 50 days, and a large number of complete plants can be obtained within 4-6 months, so that large-scale industrial seedling breeding of bletilla striata is realized. The method for rapidly breeding seedlings by using bletilla striata tubers is simple, and the seedling breeding cost is reduced greatly. By adopting the method, quick budding and high reproduction rate are realized, the method is simple, tissue culture equipment is not required to be added, the production cost is low, batch production is realized and the application value is high.

Description

technical field

[0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a propagation method for rapidly cultivating seedlings by using bletilla striata tubers. Background technique

[0002] Bletilla striata, also known as Baiji and Ruolan, is a kind of Orchidaceae Baiji, a perennial herb with pseudobulbs, rhizomes, white, plump, and finger-like divergences. Stem stout, erect, 30-60 cm tall. Leaves 3-6, lanceolate or broadly lanceolate, apex acuminate, base sheath-like clasping. Raceme terminal, sparse, with 3-8 flowers, large and beautiful, purple-red. Petals 3, lip obovate-oblong, deeply 3-lobed, middle lobe with wavy teeth on edge, side lobe partially covering stamen. Sepals 3, petallike. Capsule, terete, above, with 6 longitudinal ribs prominent. The seeds are as small as dust. The flowering period is from late April to late May, and the fruit ripening period is mid-to-late November. There is a variety of Bletil...

Examples

Embodiment 1

[0035] Select the fresh tubers of Bletilla striata harvested on October 15, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 80%, maintain the temperature at about 23°C, turn it halfway 6 times, and process it for 15 days to obtain a single tuber of bletilla striata that turns green. Cut each green bletilla striata tuber crosswise with a knife into small pieces about 2cm thick (divide into small ellipsoidal pieces at the vertical angle of the tuber growth direction of bletilla striata, each single tuber can be divided into 3 to 8 small pieces) , and then mixed with 4 kg of sterilized granular activated carbon of about 5 mesh and 10 kg of bletilla striata small pieces, and then sprayed the aqueous solution containing 2 g / L of 6-BA, 0.3 g / L NAA, and 3% carbendazim once every other day, Spray...

Embodiment 2

[0037] Select the fresh tubers of Bletilla striata excavated on September 15, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 8% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 75%, maintain the temperature at about 21°C, stir 8 times halfway, and process for 18 days to obtain a single tuber bletilla striata that turns green. Each greening Bletilla striata tuber was cut crosswise with a knife and divided into 10 kg of small pieces with a thickness of 1.5 cm. After mixing with 6 kg of granulated activated carbon of about 5 mesh after sterilization, spray 6-BA containing 2 g / L, 0.2 g / LNAA, 4% carbendazim aqueous solution once, spray 15kg / time per 100kg Bletilla striata tubers, maintain relative humidity at 75%, light intensity 1000-1200LX, light 14 hours a day, temperature at 20-25°C, 28 days to sprout body. Spray 100kg of bletilla striata...

Embodiment 3

[0039] Select the fresh tubers of Bletilla striata excavated on July 10, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% to 10% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 80%, maintain the temperature at about 25°C, turn it halfway 7 times, and process it for 20 days to obtain a single tuber of bletilla striata that turns green. Cut each greening Bletilla striata tuber crosswise with a knife into 20kg of small pieces with a thickness of 1.5cm, mix them with 7kg of granular activated carbon of about 5 mesh after sterilization, and spray 6-BA containing 3g / L, 0.4g every other day / LNAA, 2% carbendazim aqueous solution once, spray 8kg / time per 100kg bletilla striata tubers, maintain relative humidity at 75%, light intensity 1000-1200LX, light for 14 hours a day, temperature at 20-25°C, sprouts in 30 days . After the bud-inducing tre...

technical field

[0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a propagation method for rapidly cultivating seedlings by using bletilla striata tubers. Background technique

[0002] Bletilla striata, also known as Baiji and Ruolan, is a kind of Orchidaceae Baiji, a perennial herb with pseudobulbs, rhizomes, white, plump, and finger-like divergences. Stem stout, erect, 30-60 cm tall. Leaves 3-6, lanceolate or broadly lanceolate, apex acuminate, base sheath-like clasping. Raceme terminal, sparse, with 3-8 flowers, large and beautiful, purple-red. Petals 3, lip obovate-oblong, deeply 3-lobed, middle lobe with wavy teeth on edge, side lobe partially covering stamen. Sepals 3, petallike. Capsule, terete, above, with 6 longitudinal ribs prominent. The seeds are as small as dust. The flowering period is from late April to late May, and the fruit ripening period is mid-to-late November. There is a variety of Bletil...

Examples

Embodiment 1

[0035] Select the fresh tubers of Bletilla striata harvested on October 15, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 80%, maintain the temperature at about 23°C, turn it halfway 6 times, and process it for 15 days to obtain a single tuber of bletilla striata that turns green. Cut each green bletilla striata tuber crosswise with a knife into small pieces about 2cm thick (divide into small ellipsoidal pieces at the vertical angle of the tuber growth direction of bletilla striata, each single tuber can be divided into 3 to 8 small pieces) , and then mixed with 4 kg of sterilized granular activated carbon of about 5 mesh and 10 kg of bletilla striata small pieces, and then sprayed the aqueous solution containing 2 g / L of 6-BA, 0.3 g / L NAA, and 3% carbendazim once every other day, Spray...

Embodiment 2

[0037] Select the fresh tubers of Bletilla striata excavated on September 15, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 8% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 75%, maintain the temperature at about 21°C, stir 8 times halfway, and process for 18 days to obtain a single tuber bletilla striata that turns green. Each greening Bletilla striata tuber was cut crosswise with a knife and divided into 10 kg of small pieces with a thickness of 1.5 cm. After mixing with 6 kg of granulated activated carbon of about 5 mesh after sterilization, spray 6-BA containing 2 g / L, 0.2 g / LNAA, 4% carbendazim aqueous solution once, spray 15kg / time per 100kg Bletilla striata tubers, maintain relative humidity at 75%, light intensity 1000-1200LX, light 14 hours a day, temperature at 20-25°C, 28 days to sprout body. Spray 100kg of bletilla striata...

Embodiment 3

[0039] Select the fresh tubers of Bletilla striata excavated on July 10, remove the fibrous roots, separate each tuber to form a single tuber, and wash it with 5% to 10% carbendazim solution. Continuously irradiate for 24 hours with illuminance of 1000-2000LX, maintain the relative humidity of the environment at about 80%, maintain the temperature at about 25°C, turn it halfway 7 times, and process it for 20 days to obtain a single tuber of bletilla striata that turns green. Cut each greening Bletilla striata tuber crosswise with a knife into 20kg of small pieces with a thickness of 1.5cm, mix them with 7kg of granular activated carbon of about 5 mesh after sterilization, and spray 6-BA containing 3g / L, 0.4g every other day / LNAA, 2% carbendazim aqueous solution once, spray 8kg / time per 100kg bletilla striata tubers, maintain relative humidity at 75%, light intensity 1000-1200LX, light for 14 hours a day, temperature at 20-25°C, sprouts in 30 days . After the bud-inducing tre...