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A kind of method for raising seedlings of Aescin

A technology of Aescinus aesculus and rhizomes, which is applied in the field of rapid induction of seedlings of Aescinus aescini, can solve the problems of difficult seedling propagation, high dormancy, and slow germination, so as to quickly adapt to the natural environment and ensure Genetic stability, the effect of promoting bud production

Active Publication Date: 2017-10-27
江西厚达科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some key technologies that need to be overcome in the artificial cultivation of Aescin
[0003] Aescinus aesculus has biological characteristics such as high degree of dormancy, slow germination, and low emergence rate, so it is difficult to propagate seeds and seedlings; rhizome propagation is not only due to the use of vegetative bodies for reproduction, but also the large demand for Aescinus aesculus rhizomes, which makes wild resources sharp. and it will degrade the germplasm and reduce the stress resistance; so far, no satisfactory results have been obtained in the tissue culture of Aescin
[0015] So far, there have been no reports at home and abroad about drug-induced formation of multiple independent buds directly targeting the rhizomes of Aescinus aesculus on ordinary seedling beds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Choose the fresh rhizomes of Aesculus aesculata excavated on January 20, and wash them with 7% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots, and the rhizome without bud head is used for medicinal purposes. Cut 10 kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix them with 15 kg of sterilized 5-mesh granular activated carbon, and keep the relative humidity at 75% at 3°C ​​for 15 days at low temperature . After the low-temperature treatment finishes, spray the germ growth-promoting solution once every three days, the germ-promoting solution is the aqueous solution containing 6-BA, 0.2g / L NAA, 0.05g / L GA3, 4% carbendazim of 3g / L, Every 100kg of Aesculus chinensis rhizome is sprayed with 10kg / time of the bud growth promoting solution, the relative humidity is maintained at 80%, the light intensity is 400-600LX, the light is 10...

Embodiment 2

[0038] Choose the fresh rhizome of Aesculus chinensis excavated on February 5, and wash it with 8% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. Cut 10kg of each Aescin flower bud head vertically into 4 to 8 small pieces, and then mix them with 16kg of sterilized 4 mesh granular activated carbon, then maintain the relative humidity at 80% at 4°C, and carry out low temperature treatment for 18 days . After the low-temperature treatment finishes, spray 1.2kg of a germ growth-promoting solution every three days. Aqueous solution, maintain relative humidity at 80%, light intensity 300-400LX, 10 hours of light per day, temperature at 19°C, sprouts in 25 days. After the bud grows, it is processed three times with the solution for cultivating roots, and the three-time treatment interval is 4 days. The solution for cultivating roots is sprayed with 1.8kg / time ...

Embodiment 3

[0040] Choose the fresh rhizomes of Aesculus aesculi that was excavated on January 20, and wash them with 9% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. Cut 10kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix them with 20kg of sterilized 3-mesh granular activated carbon, keep the relative humidity at 75% at 6°C, and carry out low-temperature treatment for 20 days . After the low temperature treatment, spray the germ growth-promoting solution once every three days. The germ-promoting solution is an aqueous solution containing 2.5g / L 6-BA, 0.3g / L NAA, 0.07g / L GA3, 4% carbendazim Spray 13kg of bud growth-promoting solution per 100kg of Aesculus chinensis rhizome, maintain relative humidity at 85%, light intensity of 400-600LX, 10h of light per day, temperature at 16°C, and sprouts in 32 days. After the buds grow out, treat them ...

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Abstract

The invention discloses a seedling method for paris polyphylla. A large number of complete plants can be obtained by breaking dormancy of latent buds at a low temperature and then carrying out shoot-inducing and root-inducing treatment, so that large-scale industrial breeding of the paris polyphylla is achieved. According to the seedling method, the inhibiting effect of various latent buds on a single rootstock of the paris polyphylla is completely avoided; the propagation coefficient is increased to the maximal extent; the hereditary stability of the paris polyphylla variety is fully ensured; and meanwhile, the phenomenon that variation of seeds and tissue culture seedlings is easily generated is avoided. Compared with other propagation methods employing the rootstocks, the seedling method has the advantages that completely separate plants can be formed; and seedlings with a plurality of buds can also be formed.

Description

technical field [0001] The invention relates to the technical field of medicinal plant seedling cultivation, in particular to a propagation method for rapidly inducing seedlings of Aesculus aescini. Background technique [0002] Aescin is a perennial herbaceous plant, also known as unipedal lotus, one arrow, grass river car and so on. Wild Aescin is distributed in Hunan, Yunnan, Sichuan, Guizhou and other provinces. The main production areas are Xiangxi in Hunan, Central Yunnan, Western Yunnan and Northwestern Yunnan. It grows at an altitude of 1400-3100m, with an annual average temperature of 10 ~ 13 ℃, annual rainfall 850 ~ 1200mm in cool mountainous areas, mostly in evergreen broad-leaved forests, coniferous and broad-leaved mixed forests, damp places on hillsides and shrublands. Aescin germinates and grows after 5 to 6 years from the seed, and then enters the reproductive growth period. Aescin is used as medicine with rhizomes and harvested in autumn. It is slightly c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/06
CPCA01G7/06A01G22/00
Inventor 向君民
Owner 江西厚达科技有限公司
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