Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata

Abstract

The invention provides a rapid breeding method for bletilla striata. Bletilla striata seedlings are obtained by the steps of processing of a seedling culture bed, selection and preprocessing of explants, induction of cluster buds, culture of roots of the bletilla striata seedlings, division culture of the bletilla striata, final-period management and the like. The method has the advantages of high suitability for industrial production, excellent formula effect, high bud ratio, short culture time, vigorous growth of plants, high transplanting survival rate, strong operability, high application value and the like and has the characteristics of low investment and high output.

Description

technical field

[0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a breeding and seedling breeding method for rapidly inducing seedlings by making full use of epidermal tissue on bletilla striata tubers. Background technique

[0002] Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption exceeds the regeneration capacity of natural resources, it will inevitably lead to endangered or even extinction of species. Simultaneously along with the day by day destruction of natural ecological environment, also further lead to the scarcity of medicinal plant resources.

[0003] Bletilla striata, also known as Baiji and...

Examples

Embodiment 1

[0030] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 4cm, and the carbendazim of wine spray 1%, puts nutrient soil on silt soil, and nutrient soil layer height is 6cm.

[0031](2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 20 minutes, place it in 75% alcohol for 30 seconds, and cut off the discolored part of the explant for later use.

[0032] (3) Induction of clustered buds: Cut the small tubers of Bletilla striata two or three times with a knife to a depth of 1-3.5 cm, and add them to the seedling induction solution for 8 minutes, which contains 0.4% gibberellin and 0.5% carbendazim , 0.8% 6-benzylaminoadenine. Put the small tubers of Bletilla striata flatly on the s...

Embodiment 2

[0037] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 6cm, and the carbendazim of wine spray 1%, puts nutrient soil on silt soil, and nutrient soil layer height is 8cm.

[0038] (2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 30 minutes, place it in 75% alcohol for 30 seconds, and cut off the discolored part of the explant for later use.

[0039] (3) Cluster bud induction: Cut the small tubers of Bletilla striata two to three times with a knife to a depth of 1-3.5 cm, and add them to the seedling induction solution for 10 minutes, which contains 0.5% gibberellin and 0.5% carbendazim , 1.5% 6-benzylaminoadenine. Put the small tubers of Bletilla striata flatly on the seedl...

Embodiment 3

[0044] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 4cm, and the carbendazim of 1% is sprayed with wine, puts nutrient soil on silt soil, and nutrient soil layer height is 7cm.

[0045] (2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 15 minutes, place it in 75% alcohol for 25 seconds, and cut off the discolored part of the explant for later use.

[0046] (3) Induction of clustered buds: cut the small tubers of Bletilla striata two to three times with a knife on one side, and the depth reaches 1-3.5cm, and add them to the seedling induction solution for 10min, which contains 0.5% GA (gibberellin), 0.5% Carbendazim, 1.2% 6-benzylaminoadenine. Place the small tubers of Bl...

technical field

[0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a breeding and seedling breeding method for rapidly inducing seedlings by making full use of epidermal tissue on bletilla striata tubers. Background technique

[0002] Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption exceeds the regeneration capacity of natural resources, it will inevitably lead to endangered or even extinction of species. Simultaneously along with the day by day destruction of natural ecological environment, also further lead to the scarcity of medicinal plant resources.

[0003] Bletilla striata, also known as Baiji and...

Examples

Embodiment 1

[0030] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 4cm, and the carbendazim of wine spray 1%, puts nutrient soil on silt soil, and nutrient soil layer height is 6cm.

[0031](2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 20 minutes, place it in 75% alcohol for 30 seconds, and cut off the discolored part of the explant for later use.

[0032] (3) Induction of clustered buds: Cut the small tubers of Bletilla striata two or three times with a knife to a depth of 1-3.5 cm, and add them to the seedling induction solution for 8 minutes, which contains 0.4% gibberellin and 0.5% carbendazim , 0.8% 6-benzylaminoadenine. Put the small tubers of Bletilla striata flatly on the s...

Embodiment 2

[0037] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 6cm, and the carbendazim of wine spray 1%, puts nutrient soil on silt soil, and nutrient soil layer height is 8cm.

[0038] (2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 30 minutes, place it in 75% alcohol for 30 seconds, and cut off the discolored part of the explant for later use.

[0039] (3) Cluster bud induction: Cut the small tubers of Bletilla striata two to three times with a knife to a depth of 1-3.5 cm, and add them to the seedling induction solution for 10 minutes, which contains 0.5% gibberellin and 0.5% carbendazim , 1.5% 6-benzylaminoadenine. Put the small tubers of Bletilla striata flatly on the seedl...

Embodiment 3

[0044] (1) The processing of nursery bed: silt is placed on lowest layer, and silt layer height is 4cm, and the carbendazim of 1% is sprayed with wine, puts nutrient soil on silt soil, and nutrient soil layer height is 7cm.

[0045] (2) Selection and pretreatment of explants: select the buds on the pseudobulbs of Bletilla striata as culture materials, remove the root hairs of Bletilla striata tubers, then separate the small tubers of Bletilla striata, wash them with detergent and tap water, and put them under running water After rinsing for 15 minutes, place it in 75% alcohol for 25 seconds, and cut off the discolored part of the explant for later use.

[0046] (3) Induction of clustered buds: cut the small tubers of Bletilla striata two to three times with a knife on one side, and the depth reaches 1-3.5cm, and add them to the seedling induction solution for 10min, which contains 0.5% GA (gibberellin), 0.5% Carbendazim, 1.2% 6-benzylaminoadenine. Place the small tubers of Bl...