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Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition

A technology of Clover and its composition, which is applied in the field of the composition and its preparation obtained by fermenting Clover with edible and medicinal fungi, can solve the problems of increased processing steps, low activity, low content of active ingredients, etc., and achieve the highest content and activity High, increased content and activity, the effect of increased content

Active Publication Date: 2016-05-11
杭州林悦农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Clover contains active ingredients such as polysaccharides, flavonoids, and polyphenols, as well as nutritional ingredients such as starch and protein. In the traditional extraction and separation process of active ingredients, starch, protein, and irritating ingredients will be extracted together, which increases the separation. Processing steps that result in lower levels of active ingredients and less activity in the final product

Method used

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  • Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition
  • Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition
  • Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Material preparation

[0054] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0055] Inoculate the Agaricus Agaricus strains preserved on the slope and the Streptomyces strains preserved on the slope respectively on the PDA plate medium, and carry out activation culture. Later Streptomyces species.

[0056] The composition of liquid MRS medium is: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, Tween-801.0ml, dihydrogen phosphate Potassium 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g and distilled water 1.0 liter.

[0057] The fermentation basal medium is composed of the following components in mass percentage: glucose 1%, K 2 HPO 4 0.1%, MgSO 4 0.05% and the balance water.

[0058] 1 LPBS buffer: 0.27g of potassium dihydrogen phosphate, 1.42g of disodium hydrogen phosphate, 8g of sodium chloride...

Embodiment 2

[0072] 1. Material preparation

[0073] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0074] The Agaricus Agaricus strains preserved on the slope and the Streptomyces strains preserved on the slope were respectively inoculated on the PDA plate medium for activation culture, the culture temperature was 20°C, and the culture time was 25 days to obtain activated Agaricus Agaricus strains and activated mushroom strains respectively. Later Streptomyces species.

[0075] The fermentation basal medium is composed of the following components in mass percentage: glucose 3%, K 2 HPO 4 0.2%, MgSO 4 0.1% and the balance water.

[0076] Liquid MRS medium and PBS buffer are the same as in Example 1.

[0077] Two, the preparation of fermented products of Clover

[0078] (1) Take 1cm 2 The size-activated Agaricus Agaricus strains were inoculated into 1L liquid MRS medium and cultured at 20°C for 8 days,...

Embodiment 3

[0090] 1. Material preparation

[0091] PDA plate medium, liquid MRS medium and PBS damping fluid are the same as embodiment 1.

[0092] Inoculate the Agaricus Agaricus strains preserved on the slope and the Streptomyces strains preserved on the slope respectively on the PDA plate medium, and carry out activation culture, the culture temperature is 30°C, and the culture time is 3 days, and the activated Agaricus Agaricus strains and activated Later Streptomyces species.

[0093] The fermentation basal medium is composed of the following components in mass percentage: glucose 2%, K 2 HPO 4 0.15%, MgSO 4 0.08% and the balance water.

[0094] Two, the preparation of fermented products of Clover

[0095] (1) Take 4cm 2 The size-activated Agaricus Agaricus strains were inoculated into 1L liquid MRS medium and cultured at 30°C for 2 days, and the culture solution was centrifuged at 6000rpm for 10 minutes to collect the first bacteria; the first bacteria were suspended in a bil...

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Abstract

The invention discloses a composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and a preparation method of the composition. The composition comprises a tetrastigma hemsleyanum diels et gilg. fermentation subcritical extract I and a streptomycete fermentation product III, and has a favorable function of reducing blood lipid. The preparation method comprises the following steps of inoculating Brazilian mushroom liquid seed fluid into tetrastigma hemsleyanum diels et gilg. solid state fermentation culture mediums for culturing, wherein the tetrastigma hemsleyanum diels et gilg. solid state fermentation culture mediums contain tetrastigma hemsleyanum diels et gilg. and bletilla striata, and then placing the cultured Brazilian mushroom liquid seed fluid in alternating magnetic field environment for culturing once again so as to obtain tetrastigma hemsleyanum diels et gilg. solid state fermentation products; then performing subcritical water extraction so as to obtain the tetrastigma hemsleyanum diels et gilg. fermentation subcritical extract I; inoculating streptomycete seed fluid into streptomycete liquid fermentation culture mediums for culturing so as to obtain the streptomycete fermentation products III, wherein the streptomycete liquid fermentation culture mediums contain extraction remnants II. According to the method disclosed by the invention, nutrient components and effective components in the tetrastigma hemsleyanum diels et gilg. can be effectively biodegraded and utilized, and the increase of the content and the activity of active components in finished products is facilitated.

Description

technical field [0001] The invention relates to the field of processing and utilization of cloverleaf, in particular to a composition obtained by fermenting cloverleaf with edible and medicinal fungi and a preparation method thereof. Background technique [0002] Clover (Tetrastigmahemsleyanum DielsetGilg) is a plant of the genus Cliff, alias: golden thread gourd, stone mouse, snake aconite, belongs to Rhamnaceae, and is a herbaceous vine of Vitis vinaceae. Scientific name: Cliff clover clover, is a traditional green herbal medicine in Zhejiang, Fujian and other regions, and the medicinal effect of underground tubers and fruits is the best. It has been used for a long time to treat tumors in Zhejiang folks. Clover is a local medicinal material, which is extremely rare. It is distributed in Zhejiang, Jiangxi, Fujian, Anhui, Guangdong, Guangxi, Yunnan and other places. Clover has the functions of cooling, regulating qi, and promoting blood circulation. , Spleen, detoxificatio...

Claims

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Application Information

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IPC IPC(8): A23L33/00A23L31/00A61K36/87A61K36/899A61P3/06C12N1/20C12N1/14C12R1/465
CPCA23V2002/00A61K36/87A61K36/899A61K2236/19C12N1/14C12N1/20A61K2300/00A23V2200/3262A23V2250/208
Inventor 程俊文贺亮程诗明杨华卢庭高胡传久魏海龙
Owner 杭州林悦农业科技有限公司
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