Induction and propagating method of Tetrastigma hemsleyanum Diels et Gilg adventitious root
A cloverleaf and induction medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as bottlenecks in the production of gourd tuberous roots and tuberous roots, and achieve the effect of solving the problem of contradiction between supply and demand
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[0019] The preferred embodiments of the present invention will be described in detail below.
[0020] The present embodiment trefoil adventitious root induction and propagation method, it carries out as follows:
[0021] (1) Induction of callus: rinse the leaves extracted from the Clover plant, sterilize with ethanol on the ultra-clean workbench and transfer to mercuric chloride for disinfection, then rinse with sterile water for 5 to 6 times, Cut into about 0.5cm×0.5cm in size, put it face up on the induction medium, and culture in the dark at 27±1°C for 30 days. In this step, the concentration of ethanol is 75%, soak the leaves for 30 seconds, transfer to 0.1% mercuric chloride for immersion and disinfection for 10 minutes, and the induction medium formula is MS+30g L -1 Sucrose+2.0mg·L -1 6-BA+0.2mg·L -1 NAA+6.5g·L -1 agar.
[0022] (2) Proliferation of callus: Cut the callus obtained in step (1) into small pieces on an ultra-clean workbench, inoculate on the prolifera...
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