Tetrastigma hemsleyanum Diels et Gilg twig single-bud cutting propagation method

Abstract

The invention discloses a tetrastigma hemsleyanum Diels et Gilg twig single-bud cutting propagation method. The method comprises the following steps that 1, cuttings are cut, wherein annual tender branches of tetrastigma hemsleyanum Diels et Gilg are cut and are cut into single-bud cuttings with leaves, the length of the lower ends of the cuttings is 4-5 cm, and the upper ends of the cuttings are 2 cm away from single buds; 2, the cuttings are processed, wherein the cuttings are dipped in a rooting conditioning solution for 15-60 seconds, the concentration of the rooting conditioning solution is 50-150 mg/L, and the rooting conditioning solution is at least one of NAA, IBA and IAA; then the cuttings are put in a matrix through cuttage, wherein the matrix contains perlite and also contains at least one of turf and vermiculite, and the cuttage depth makes the single buds rightly exposed out of the matrix; 3, management is performed after cuttage, wherein timely spraying is performed for 30 minutes after cuttage. By adopting the method, the influence of the matrix, the types and combinations of hormones and treatment time on the survival rate of tetrastigma hemsleyanum Diels et Gilg single-bud twig seedlings raised by cuttage is researched, preferred cultivation conditions are provided for quick propagation of tetrastigma hemsleyanum Diels et Gilg seedlings, and sustainable supply of tetrastigma hemsleyanum Diels et Gilg resources is achieved.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, in particular to a method for single-bud propagation of clover twigs. Background technique [0002] Clover, the scientific name is Cliff climbing vine, also known as snake monkshood, stone spore, stone monkey, stone mouse, and golden thread hanging gourd. It is a perennial vine of the family Vitis genus. Rare medicinal plants are mainly distributed in more than 10 provinces including Zhejiang, Jiangxi, Fujian, Hunan, Hubei, Guangdong, and Guangxi (Peng Xin, Ji Qingyong, Liang Yaqing, Zhang Yujiong, Lou Tianling. The rare medicinal plant Clover Research status of germplasm resources [J]. Chinese Modern Traditional Chinese Medicine, 2016, 18(8): 1088-1092), growing in hillside shrubs, valleys, In crevices of rocks under the forest by the stream (Wei Shugen, Dong Qingsong, Wei Ying. et al. Investigation and research on the resources of the endangered and rare Chinese herbal medicine C...

AI Technical Summary

Problems solved by technology

[0005] Due to the late start of the research on artificial cultivation of clover, its artificial cultivation technology system is immature and cannot be popularized and applied, resulting in the stagnation of artificial cultivation of clover and the inability to expand the planting area

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