Tetrastigma hemsleyanum Diels et Gilg root tuber polysaccharide with anti-pyretic and anti-inflammatory functions and application of Tetrastigma hemsleyanum Diels et Gilg root tuber polysaccharide
An anti-inflammatory effect, the technology of cloverleaf, applied in the field of medicine, can solve the problem that there is no report on the antipyretic and anti-inflammatory effect of cloverleaf polysaccharide, and achieve a significant antipyretic and anti-inflammatory effect
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[0024] Preparation of trifolium root polysaccharide with antipyretic and anti-inflammatory effects:
[0025] (1) Preparation of crude polysaccharides from Pseudomonas clover root: Take Pseudomonas clover root and dry it in an oven at 35-65°C, dry it to a constant weight and then pulverize it, mix the pulverized product with double distilled water according to the ratio of 1g: 20mL of material to liquid, and heat to reflux Extract 3 times, 1h each time. After filtration, the extracts were combined for 3 times, concentrated by distillation under reduced pressure, 3 times the volume of 95% ethanol was added, after standing overnight, centrifuged at 4200rpm for 15min at high speed, the precipitate was dissolved in water, and then ethanol precipitation was carried out again, centrifuged to obtain the crude polysaccharides.
[0026] (2) Determination of crude polysaccharide content by phenol-sulfuric acid method:
[0027] ①Preparation of standard solution: D-glucose standard produ...
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