Method for determining content of epicatechin in Rubuscochinchinensis

A technique for epicatechin and determination method, applied in the field of epicatechin content determination, can solve different problems, achieve the effects of strong specificity, promotion of industrialized utilization, and good separation

Pending Publication Date: 2021-11-30
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different components and contents contained in different plants or medicinal materials, even the content determination methods of the same substance may be affected by interfering components and contents.

Method used

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  • Method for determining content of epicatechin in Rubuscochinchinensis
  • Method for determining content of epicatechin in Rubuscochinchinensis
  • Method for determining content of epicatechin in Rubuscochinchinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for determining the content of epicatechin in snake gluten, using high performance liquid chromatography to determine the content of epicatechin in snake gluten, specifically comprising the following steps:

[0039] (1) Preparation of reference substance solution: take epicatechin reference substance and dissolve it in methanol to make reference substance solution;

[0040] (2) Preparation of the testing solution: get the medicinal material powder of the root of the snakehead root and dissolve it in ethanol to obtain the medicinal solution, weigh it, then filter it through ultrasonic treatment, let it cool, and weigh it, and the filtered filtrate is the testing product solution;

[0041] (3) Get reference substance solution and need testing solution and inject high performance liquid chromatography, detect the content of epicatechin; Described high performance liquid chromatography condition is:

[0042] Chromatographic column: Hanbon lichrospher C18 250×4.6mm...

Embodiment 2

[0051] A method for determining the content of epicatechin in snake gluten, using high performance liquid chromatography to determine the content of epicatechin in snake gluten, specifically comprising the following steps:

[0052] (1) Preparation of reference substance solution: take epicatechin reference substance and dissolve it in methanol to make reference substance solution;

[0053] (2) Preparation of the testing solution: get the medicinal material powder of the root of the snakehead root and dissolve it in ethanol to obtain the medicinal solution, weigh it, then filter it through ultrasonic treatment, let it cool, and weigh it, and the filtered filtrate is the testing product solution;

[0054] (3) Get reference substance solution and need testing solution and inject high performance liquid chromatography, detect the content of epicatechin; Described high performance liquid chromatography condition is:

[0055] Chromatographic column: Hanbon lichrospher C18 250×4.6mm...

Embodiment 3

[0064] A method for determining the content of epicatechin in snake gluten, using high performance liquid chromatography to determine the content of epicatechin in snake gluten, specifically comprising the following steps:

[0065] (1) Preparation of reference substance solution: take epicatechin reference substance and dissolve it in methanol to make reference substance solution;

[0066] (2) Preparation of the testing solution: get the medicinal material powder of the root of the snakehead root and dissolve it in ethanol to obtain the medicinal solution, weigh it, then filter it through ultrasonic treatment, let it cool, and weigh it, and the filtered filtrate is the testing product solution;

[0067] (3) Get reference substance solution and need testing solution and inject high performance liquid chromatography, detect the content of epicatechin; Described high performance liquid chromatography condition is:

[0068] Chromatographic column: Hanbon lichrospher C18 250×4.6mm...

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Abstract

The invention discloses a method for determining the content of epicatechin in Rubuscochinchinensis, which adopts high performance liquid chromatography to determine the content of epicatechin in Rubuscochinchinensis. The high performance liquid chromatography conditions are as follows: the chromatographic column is Hanbonlichrospher C18; a mobile phase is acetic acid water-acetonitrile; the flow velocity is 0.8 to 1.2 ml / min; the time is 10 to 15 minutes; the detection wavelength is 280 nm; the column temperature is 35-42 DEG C; and the sample size is 0.002 to 0.012 ml. The method disclosed by the invention is precise, rapid and high in specificity, and a theoretical basis and an experimental basis are laid for establishing a quality standard of the Rubuscochinchinensis; the method has important significance in expanding medicinal resources of the Rubuscochinchinensis and promoting industrial utilization of the Rubuscochinchinensis.

Description

technical field [0001] The application relates to the technical field of traditional Chinese medicine, in particular to a method for determining the content of epicatechin in snake gluten. Background technique [0002] Serpentis sinensis is a commonly used Chinese herbal medicine in Hainan Province. It is recorded in "Hainan Botanical Illustrated Records". The root of snakeskin sinensis is the medicinal part of Rubuscochinchinensis. It is mainly distributed in low-altitude mountains, hills and roads in Hainan and Guangdong Province. In the sparse forest. Its taste is slightly pungent, sweet, astringent, warm. Have expelling wind and relieving pain, dispelling stagnation, detoxification. For the treatment of rheumatic low back pain, diabetes, intestinal gas, hemorrhoids, and eczema. There are few researches on the chemical constituents of snake gluten. Its congener plant, Rubus parvifolius L, mainly contains tannin, flavonoid glycosides, sugars, phenols, and amino acids. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 李永辉吴娇霞吴毓皇徐晗李立言
Owner HAINAN MEDICAL COLLEGE
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