Method for simultaneously detecting multiple nucleoside components in antler

Abstract

The invention discloses a method for simultaneously detecting various nucleoside components in antler, which comprises the following steps: preparation of a test solution: taking antler powder, adding water, oscillating and uniformly mixing, refrigerating and standing, ultrasonically extracting, centrifuging, taking supernate and filtering to obtain the test solution; preparing a mixed reference substance solution, namely precisely weighing a proper amount of cytidine, uracil, hypoxanthine, uridine, thymine, 2 '-deoxyuridine, inosine, guanosine, 2'-deoxyinosine, 2 '-deoxyguanosine, thymidine, guanine and xanthine, dissolving and diluting to prepare the mixed reference substance solution; and performing UPLC content detection: performing ultra-high performance liquid chromatography detection on the test solution and the mixed reference solution. The detection method disclosed by the invention is simple and convenient to operate, high in sensitivity and good in stability, repeatability and precision, nucleoside components in the antler can be comprehensively analyzed, and a basis is provided for development and utilization of commercial antler instead of medicinal antler.

Description

technical field [0001] The invention belongs to the technical field of quality detection of traditional Chinese medicines, in particular to a method for simultaneously detecting multiple nucleoside components in antlers. Background technique [0002] As one of the traditional precious traditional Chinese medicines in my country, antlers have a long history of use and were first recorded in "Shen Nong's Materia Medica". The antlers included in the 2020 edition of the Pharmacopoeia of the People's Republic of China are the ossified horns of the red deer Cervus elaphus Linnaeus or the sika deer Cervus Nippon Temminck or the horn base dropped in the second spring after sawing velvet, which has a warm kidney yang , Strengthening the muscles and bones, reducing swelling and blood flow. Modern medicine believes that antlers are rich in nutrients, including amino acids, polypeptides, nucleosides, polysaccharides, lipids, sex hormones and mineral elements, etc. , anti-inflammatory ...

AI Technical Summary

Problems solved by technology

The pharmacological effects and chemical components of these antlers are similar to those of medicinal antlers, and it is feasible to use them as a substitute resource for medicinal antlers, but there is no scientific evaluation index for medicinal use

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