A kind of total flavonoids of pecan leaf extract with uric acid-lowering effect and its preparation method and application

A technology of hickory leaves and extracts, applied in the field of medicine, can solve problems such as hyperuricemia that have not been seen, and achieve the effect of reducing serum uric acid levels

Active Publication Date: 2019-09-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the treatment of hyperuricemia with the total flavonoids of hickory leaf extract

Method used

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  • A kind of total flavonoids of pecan leaf extract with uric acid-lowering effect and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0018] A preparation method of total flavonoids of hickory leaf extract with uric acid-lowering effect, comprising the following steps: (1) taking hickory nut leaves for oven drying at 35-65° C., pulverizing them to 40-80 mesh, putting them into a round-bottomed flask, Add volume percentage concentration of 70-95% ethanol aqueous solution (add 10mL per g hickory leaf pulverization), extract at 80-110°C under normal pressure for 20-100 minutes, repeat the extraction 1-3 times; then filter with filter paper and combine filtrate;

[0019] (2) the filtrate is concentrated under reduced pressure at 300 ± 100pa vacuum, so that the ethanol concentration in the filtrate is 45-65%, centrifugal (2000-4000 revs per minute, centrifugal 20 minutes) gets the supernatant, passes this supernatant SD200 macroporous adsorption resin column, the sample loading speed is 1BV / h-8BV / h, and the sample volume is 4-8BV; at 20-35°C, under normal pressure, at a flow rate of 1BV / h-8BV / h, wash with distill...

specific Embodiment 1

[0021] After hickory leaves are dried, mechanically pulverize, take by weighing hickory leaf powder 200g, add 10 times of amount (g:mL) volume percentage is 95% ethanol solution, extract 2 times, each 30min, combine filtrate, at 300 ± Concentrate under reduced pressure under a vacuum of 100 Pa to make the ethanol concentration in the filtrate 55%, centrifuge at 3000 rpm for 20 minutes, and take the supernatant. Weigh 4.0 g of SD200 aromatic macroporous adsorption resin (Zhejiang Zhengguang Industrial Co., Ltd.), put it into a chromatographic column (15mm×350mm i.d), and load the sample at a flow rate of 2BV / h, with a sample volume of 6.5BV. First eluted with 10BV of water, then eluted with 10BV of 20% ethanol solution by volume percentage, and finally eluted with 10BV of 70% ethanol solution by volume percentage, the elution rate was 4BV / h, and the 70% ethanol eluate was collected , reduced pressure and rotary evaporation to dryness to obtain the total flavonoids of hickory nu...

specific Embodiment 2

[0023] After the hickory leaves are dried, crush them mechanically, weigh 400g of hickory leaf powder, add 10 times the amount of ethanol solution with a volume percentage of 95%, extract twice, each time for 30min, combine the filtrates, and depressurize under a vacuum of 300±100pa Concentrate so that the ethanol concentration in the filtrate is 55%, centrifuge at 3000 rpm for 20 minutes, and take the supernatant. Weigh 200.0 g of SD200 macroporous adsorption resin, put it into a chromatographic column (15mm×350mm i.d), and load the sample at a flow rate of 2BV / h, with a sample volume of 6.5BV. First eluted with 10BV of water, then eluted with 10BV of 20% ethanol solution by volume percentage, and finally eluted with 10BV of 70% ethanol solution by volume percentage, the elution rate was 4BV / h, and the 70% ethanol eluate was collected , rotary evaporation under reduced pressure to dryness, to obtain the total flavonoids of hickory nut leaf extract, wherein the total content o...

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Abstract

The invention discloses pecan leaf extract total flavone with uric-acid lowering effect and a preparation method and application thereof. The pecan leaf extract total flavone contains pinostrobin, pinostrobin chalcone, chrysin and cardamonin whose total mass percentage is 50% or above. The total flavone in pecan leaf is prepared on the basis of the pecan leaf, efficacy of the pecan leaf is developed, and novel medicinal resources of the pecan leaf are expanded. The pecan leaf extract total flavone can remarkably lower serum uric acid level of model rats and can be used for medicaments and healthcare products having main functions of preventing and treating hyperuricemia.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to total flavonoids from hickory nut leaf extracts with the effect of lowering uric acid and its preparation method and application. Background technique [0002] Uric acid is a metabolite of endogenous purines and dietary purines. In the purine metabolism of the human body, ATP and others undergo a series of metabolic reactions to produce hypoxanthine and xanthine, which generate uric acid under the action of xanthine oxidase. When the purine metabolism is disordered, the blood uric acid level is too high, leading to the occurrence of hyperuricemia (Hyperuricemia), which in turn leads to the crystallization of urate and precipitation in the joints, causing gout attacks, often involving the kidneys and causing chronic interstitial nephritis and Uric acid kidney stones, and may lead to metabolic syndrome and related cardiovascular disease. In recent years, the incidence of hyperu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/52A61P19/06
Inventor 丁志山钱朝东吕敏蒋福升金波朱学鑫陈铌铍高承贤
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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