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A hickory leaf extract condyloconin with uric acid-lowering effect and its preparation method and application

A technology of hickory leaves and ballpinen, which is applied in the field of medicine, can solve problems such as hyperuricemia that have not been seen, and achieve the effect of reducing serum uric acid levels

Active Publication Date: 2018-06-15
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the treatment of hyperuricemia with hickory leaf extract ballochamin

Method used

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  • A hickory leaf extract condyloconin with uric acid-lowering effect and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] A kind of preparation method of the hickory nut leaf extract bulbocpinin that has uric acid-lowering effect, comprises the following steps: (1) take hickory nut leaf 35-65 ℃ oven-dried, pulverize to 40-80 order, put into round bottom flask , add 70-95vol% ethanol aqueous solution, extract at 80-110°C under normal pressure for 20-100 minutes, repeat the extraction 1-3 times; then filter with filter paper, and combine the filtrates;

[0020] (2) adding concentration in the filtrate is 1mol / L sodium hydroxide solution alkalization 12h, every 4ml filtrate adds 0.5-1mL concentration and is the sodium hydroxide solution of 1mol / L, adds the filtrate after sodium hydroxide solution alkalization and poly Amides (40-200 mesh) are fully mixed at a liquid-to-mass ratio of 10-80mL:1g, concentrated to dryness under reduced pressure at 55°C and 300±100pa vacuum, then directly add water to the column, at a flow rate of 5-10BV / h, sequentially Elute with water, 20vol% ethanol, 60vol% eth...

specific Embodiment 1

[0023] After hickory leaves are dried, mechanically pulverize them, weigh 200 g of hickory leaf powder, add 10 times the amount (g: mL) of 95% ethanol solution by volume, extract 2 times, each time for 30 min, combine the filtrates, The filtrate, 0.7mL of sodium hydroxide solution with a concentration of 1mol / L, was alkalized for 12h, and 100mL of the alkalized sample solution was taken, mixed with 5g of polyamide (100-200 mesh), and vacuum decompressed at 300±100pa Concentrate to dryness, put on a chromatographic column (15mm×350mm i.d), and successively elute with 10BV water, 10BV20vol% ethanol, 10BV60vol% ethanol, collect 60% ethanol eluate, and concentrate under reduced pressure at 300±100pa to Dry to obtain the crude extract of hickory nut leaves.

[0024] The obtained pecan leaf crude extract is dissolved in methanol solution, the dissolving ratio is 1g pecan leaf crude extract, 20mL methanol solution, and then an equal volume of petroleum ether is used to extract 10 tim...

specific Embodiment 2

[0025] After hickory leaves are dried, mechanically pulverize, take by weighing hickory leaf powder 400g, add 10 times of volume percentage and be 95% ethanol solution, extract 2 times, each 30min, merge filtrate, press every 4mL filtrate, 0.7mL concentration is The proportion of sodium hydroxide solution of 1mol / L, carry out alkalization for 12h, take 6000mL of the sample solution after alkalization, and 300g polyamide (100-200 mesh), fully mix, vacuum rotary evaporation to dryness, upper chromatography column ( 7.5cm×90.0cm i.d), first eluted with 10BV of water, then eluted with 10BV of 20% ethanol solution by volume percentage, and finally eluted with 10BV of 60% ethanol solution by volume percentage, and collected the 60% ethanol eluate , Concentrated to dryness under reduced pressure at 300±100pa vacuum to obtain the crude extract of pecan leaves.

[0026] The obtained pecan leaf crude extract is dissolved in methanol solution, the dissolving ratio is 1g pecan leaf crude ...

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Abstract

The invention discloses Chinese walnut leaf extract pinostrobin having a uric acid reducing effect and a preparation method thereof and application. The mass percent of pinostrobin in the Chinese walnut leaf extract pinostrobin is 90% or above. Walnut leaves serve as the basis, pinostrobin in the walnut leaves is prepared, effects of pinostrobin are developed, and new medicinal resources of the walnut leaves are expanded. The Chinese walnut leaf extract pinostrobin can remarkably reduce serum uric acid level of a model rat and can be used for making medicines and health care products having a main function of preventing hyperuricemia.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a hickory leaf extract conchapinen with the effect of lowering uric acid and its preparation method and application. Background technique [0002] Uric acid is a metabolite of endogenous purines and dietary purines. In the purine metabolism of the human body, ATP and others undergo a series of metabolic reactions to produce hypoxanthine and xanthine, which generate uric acid under the action of xanthine oxidase. When the purine metabolism is disordered, the blood uric acid level is too high, leading to the occurrence of hyperuricemia (Hyperuricemia), which in turn leads to the crystallization of urate and precipitation in the joints, causing gout attacks, often involving the kidneys and causing chronic interstitial nephritis and Uric acid kidney stones, and may lead to metabolic syndrome and related cardiovascular disease. In recent years, the incidence of hyperuricemia has i...

Claims

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Application Information

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IPC IPC(8): A61K31/352C07D311/32C07D311/40A61P19/06
CPCA61K31/352C07D311/32C07D311/40
Inventor 丁志山钱朝东蒋福升黄燕芬金波吕敏朱学鑫丁滨
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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