55 results about "L-Epicatechin" patented technology

The invention relates to the technical field of analysis and detection, in particular to a molecularly imprinted sensor for in-situ detection of epicatechin in plant leaves and a preparation and application method of the molecularly imprinted sensor. The molecular imprinting sensor for detecting epicatechin is prepared by assembling a zeolite imidazate skeleton-amide functionalized single-walled carbon nanotube composite material, amino-formyl imidazole modified reduced graphene and an MIP film on the surface of an electrode layer by layer. The high-selectivity and high-sensitivity detection on the target molecule epicatechin is realized. The technology can effectively reduce the detection cost, simplify the operation process and improve the performance of the sensor. Particularly, due to the introduction of the MIP, the sensor has the capabilities of in-situ detection and high-precision molecular recognition, can realize rapid and reliable quantitative analysis in a complex sample, and has a wide application prospect.

Provided are a use of epicatechin or a legume extract containing epicatechin for preparing a composition for tranquilizing skin, and a skin itch treatment method comprising a step of applying a cosmetic composition to the skin of an individual causing itch, the cosmetic composition being the composition for tranquilizing skin.

The invention relates to the technical field of polymer degradable materials, in particular to a biodegradable material as well as a preparation method and application thereof. The material takes modified polylactic acid as a main body, and an antioxidant, a plasticizer, a flame retardant and an inorganic filler are used as auxiliary materials in parts by weight. The method is characterized in that lactic acid and an epicatechin imidazole ammonium chloride polymer are polymerized under the catalysis of stannous octoate, and an epicatechin bioactive group is introduced into a polylactic acid main chain in a chemical bonding manner, so that the biodegradation rate, the mechanical property and the thermal stability of the material are remarkably improved. Bioactive substances and ionic groups in the material promote microbial degradation, and the material can be completely decomposed in a compost environment in a short time; meanwhile, the strength, the toughness and the high-temperature processability of the polylactic acid are effectively improved through molecular-level enhancement and an ionic cross-linking effect. The material can be widely applied to the fields of disposable tableware, food packaging films, degradable agricultural mulching films and the like.

The invention relates to the field of cosmetics, in particular to a berry composition for scalp care and a preparation method and application thereof, and the preparation method comprises the following steps: A, pretreating fruits to obtain berry freeze-dried powder; b, preparing a deep eutectic solvent; c, mixing the berry freeze-dried powder with the deep-eutectic solvent, and performing oscillation extraction to obtain a berry procyanidine crude extract; d, purifying the berry procyanidine crude extract with macroporous resin to obtain a first purified berry procyanidine product; e, removing the solvent from the first purified berry proanthocyanidins to obtain a second purified berry proanthocyanidins; and F, dissolving epicatechin, mixing the dissolved epicatechin with the second purified product of the berry procyanidine, uniformly heating in a vacuum environment, cooling after relieving the vacuum environment, mixing with amino acid hydrochloride, and preserving heat to obtain the berry extract.

The present invention discloses a method for improving the quality of Millettia reticulata, belonging to the technical field of Millettia reticulata cultivation. It applies a growth regulator in combination with spectral lighting. The growth regulator is methyl jasmonate, with a concentration of 100 - 400 μmol / L. The LED light quality of the spectral lighting is red light and blue light, and the total photon flux density of red light and blue light is 200 μmol / m 2 / s, and the light quality ratio of red light to blue light is 2 - 5:1. The method of the present invention can effectively improve the growth of Millettia reticulata seedlings, significantly increase the contents of the active ingredients total flavonoids, catechins, epicatechins and gallocatechins, especially the improvement effect of catechins is the best. In an embodiment, compared with the blank group, the contents of total flavonoids, catechins, epicatechins and gallocatechins are significantly increased by 83.05%, 314.72%, 81.03% and 179.62% respectively, and the effect is remarkable.

The invention provides a detection method of flavan-3-alcohol sulfonate and application of the detection method. The method comprises the following specific steps: preparing and separating flavane-3-alcohol sulfonate, analyzing and screening molecular characteristic correlation and mass spectrum signal response, identifying the structure of flavane-3-alcohol sulfonate, drawing a standard curve of a flavane-3-alcohol sulfonate standard product, and detecting and analyzing flavane-3-alcohol sulfonate in wine. According to the invention, the epicatechin-(4beta)-sulfonate and the epigallocatechin-(4beta)-sulfonate are obtained by inserting sulfonyl into catechin compounds for preparation and separation, and the epicatechin-(4beta)-sulfonate and the epigallocatechin-(4beta)-sulfonate are used as standard substances for detecting the flavan-3-alcohol sulfonate in the grape wine and are applied to detection and analysis of the flavan-3-alcohol sulfonate in the grape wine. The method provided by the invention has the characteristics of simple and rapid compound synthesis route, easy control of operation conditions, high identification accuracy and sensitivity and strong specificity, provides a good method reference for detection of flavan-3-alcohol sulfonate in the wine aging process, and ensures the fragrant and mellow flavor and taste of wine.

The invention discloses a method for separating and extracting epicatechin, formononin and genistin from caulis spatholobi and application of the formononin, belongs to the technical field of natural product extraction and medical application, and relates to an efficient and controllable extraction process. According to the method, the three active ingredients of epicatechin, ononin and genitin can be enriched and purified at the same time from the caulis spatholobi, the potential medicinal value of ononin in gastric cancer treatment is further revealed, and a scientific basis is provided for deep development and comprehensive utilization of the caulis spatholobi.

The invention provides application of epicatechin in preparation of a medicine for resisting frog iridovirus. Related experiments prove that the epicatechin can efficiently prevent and control infection and transmission of the frog iridovirus, so that the harm of the frog iridovirus to healthy culture of frogs such as bullfrogs can be reduced to a great extent, and high-quality ecological culture and economic benefits of the frog culture industry are promoted.

The invention relates to efficient modified resin for adsorbing epicatechin. According to the method, alkalescent resin D301G is taken as a raw material, and chloromethylation is carried out to prepare the D301G-Cl resin. The preparation method comprises the following steps: adding D301G-Cl resin and N, N-dimethylformamide into a three-necked flask with a stirrer and a thermometer, sealing a cover, soaking, and adding NaCl, p-aminobenzene sulfonic acid and NaOH into the flask; stirring the mixed solution by using a stirrer, and reacting for a period of time at constant temperature; and after the reaction is finished, filtering by adopting a filter screen with a certain mesh number, and recycling filtrate. Repeatedly washing the resin with ethanol and distilled water until the added AgNO3 solution does not precipitate; the modified resin (D301G-SO3 <->) with the sulfonic acid group is obtained. The macroporous resin contains amino groups and sulfonic acid groups, so that the adsorption of epicatechin hydroxyl groups is further enhanced on the basis of benzene ring pi-pi adsorption. The adsorption capacity of the D301G-SO3-resin is larger than that of AB-8 under the condition that the pore volume is small, the adsorption effect of the D301G-SO3-resin on epicatechin is remarkably improved, the maximum adsorption capacity is improved by one time, the maximum adsorption capacity is kept within the PH range of 7-9, and the adsorption efficiency on the epicatechin is greatly improved.

Provided herein are process steps for the synthesis of (-)-epicatechin. The synthesis method provided by the invention is divided into two stages: (1) synthesizing an epoxide intermediate and (2) synthesizing the (-)-epicatechin.

The invention provides a deep eutectic solvent and a preparation method and application thereof, the effective components of the deep eutectic solvent are composed of choline chloride and ethylene glycol, the molar ratio of choline chloride to ethylene glycol is 1: 4, the water content of the effective components is 30%, and the deep eutectic solvent can replace an organic solvent to extract total flavonoids from fructus amomi; chemical components capable of distinguishing the varieties of the fructus amomi are determined through quantitative experiments on the fructus amomi flavonoid compounds, serve as differential markers epicatechin, procyanidine B2 and procyanidine B4 for distinguishing the varieties of the fructus amomi and have very high antioxidant activity, and H2O2-induced GES-1 cell oxidative stress is relieved by removing ROS in cells and up-regulating the level of SOD and GSH-Px.

The invention relates to construction of a chinaroot greenbrier medicinal material fingerprint spectrum and application of the chinaroot greenbrier medicinal material fingerprint spectrum in medicinal material quality control. The HPLC fingerprint spectrum of the chinaroot greenbrier medicinal material has 23 characteristic fingerprint chromatographic peaks, the peak 6 is chlorogenic acid, the peak 7 is epicatechin, the peak 9 is 5-O-caffeoylshikimic acid, the peak 10 is polydatin, the peak 11 is oxyresveratrol, the peak 12 is neoastilbin, the peak 13 is astilbin, the peak 14 is neoiso-astilbin, the peak 15 is resveratrol, the peak 16 is engeletin, and the peak 17 is quercitrin. The peak 20 is kaempferol.

This invention relates to the field of polymer degradable materials technology, specifically to a biodegradable material, its preparation method, and its applications. The material is based on modified polylactic acid (PLA), supplemented by antioxidants, plasticizers, flame retardants, and inorganic fillers in specific weight proportions. Its unique feature lies in the polymerization of lactic acid and epicatechin imidazole ammonium chloride polymer under stannous octoate catalysis, using chemical bonding to introduce the bioactive groups of epicatechin into the PLA backbone, significantly improving the material's biodegradation rate, mechanical properties, and thermal stability. The bioactive substances and ionic groups in this material promote microbial degradation, achieving complete decomposition in a composting environment within a short time. Simultaneously, through molecular-level reinforcement and ionic cross-linking effects, the strength, toughness, and high-temperature processing performance of PLA are effectively improved. This material can be widely used in disposable tableware, food packaging films, and biodegradable agricultural mulch films.

The invention discloses a low caffeine nerve stimulation tea wine based on litchi wood and sandalwood and a preparation process thereof, the wine is mainly prepared by blending litchi wood barrel aged tea wine and litchi wood / sandalwood barrel aged tea wine with different baking degrees, and the mass ratio of protocatechuic acid to catechin to epicatechin in the wine body is (0.15-0.28): 1: (0.15-0.25). The mass ratio of the protocatechuic acid to the alpha-santalol to the beta-santalol is 1: (0.4-0.6), the mass ratio of the quercetin to the gallic acid to the ferulic acid is (1.7-2.5): 1: (2.5-3.5), the mass ratio of the protocatechuic acid to the alpha-santalol is (1-2.5): 1, and the total content of the protocatechuic acid, the catechin, the epicatechin, the alpha-santalol, the beta-santalol, the quercetin, the gallic acid and the ferulic acid in the wine is 60-600mg / 100mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The invention relates to the field of preparation of starch nano-particles, and particularly provides pepper starch nano-particles loaded with epicatechin and a preparation method of the pepper starch nano-particles. According to the preparation method, pepper starch is used for loading epicatechin, and contrast experiments prove that the specific process combination (debranching, stirring and homogenizing) is crucial for realizing high encapsulation efficiency. The finally prepared pepper starch nanoparticles can significantly improve the entrapment efficiency of epicatechin. Therefore, the load efficiency is higher, the waste of active ingredients is lower, and the method has a remarkable industrial cost advantage.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a construction method of a specific chromatogram of airpotato yam rhizome and a preparation thereof, and the construction method comprises the following steps: (1) preparing a test solution; (2) the test solution is taken and detected by adopting a high performance liquid chromatography, octadecylsilane chemically bonded silica is taken as a filler, a mobile phase comprises an aqueous solution containing phosphoric acid and acetonitrile, a gradient elution procedure comprises 0-3 min, 15 min, 30 min, 37 min and 40 min, and the volume percentage of acetonitrile in the mobile phase is 10%-10%-12%-27%-47%-47%. According to the method, 11 common characteristic peaks can be separated, the common characteristic peaks including epicatechin are included, the obtained characteristic spectrum is stable in baseline, good in characteristic peak shape and high in separation degree, a basis is provided for quality detection and control of airpotato yam and a preparation thereof, and airpotato yam can be clearly distinguished from airpotato yam, oriental stephania root and girald pteroxygonum root.

The present invention discloses a quality detection method for a traditional Chinese medicine compound Qingkui Yuyang Decoction. The UPLC-Q-Exactive Orbitrap-MS / MS analysis method is used to qualitatively analyze the chemical components of the six medicinal materials in the Qingkui Yuyang Decoction, namely, coptis chinensis, frankincense, sanguisorba officinalis, indigo naturalis, catechu, and dragon's blood. Then, the high performance liquid chromatography method is used to determine the contents of epicatechin, catechin, rutin, coptisine, and berberine in the Qingkui Yuyang Decoction. The UPLC-Q-Exactive Orbitrap-MS / MS analysis method has the advantages of high resolution, good mass accuracy, and strong qualitative ability, and can perform extensive and accurate detection of the chemical components in complex traditional Chinese medicine compounds. The high performance liquid chromatography can provide detection results with high accuracy, high sensitivity, and good repeatability, is simple and easy to operate, and has passed methodological verification, with good linearity. The precision, stability, repeatability, and recovery rate all meet the requirements. The combination of qualitative and quantitative analysis lays the foundation for the establishment of the quality standard for Qingkui Yuyang Decoction.

The present disclosure is related to dietary supplements. For example, this disclosure relates to compositions that include, one or more agents that decrease the production of tumor necrosis factor-alpha (TNF-α), decrease the CCL2, and decrease the interleukin-1 beta (IL-1β) responsible for low grade chronic inflammation.In some embodiments, the composition comprises: epicatechin present in an amount of about 0.25% to about 1% w / w of the composition; EGCG present in an amount of about 0.01% to about 0.1% w / w of the composition; lycopene present in an amount of about 1% to about 3% w / w of the composition; quercetin present in an amount of about 0.1% to about 1% w / w of the composition; luteolin present in an amount of about 0.1% to about 1% w / w of the composition; vitamin A present in an amount of about 1% to about 7.5% w / w of the composition; vitamin C present in an amount of about 40% to about 77% w / w of the composition; and vitamin E present in an amount of about 15% to about 40% w / w of the composition. Such compositions are useful for improving LGCI by decreasing TNF-α, CCL2, and IL-1β concentration.

The present disclosure relates to compositions of biocompounds derived from cacao, mainly aromatic compounds such as flavanols and methylxanthines in the form of suspended particles, and to methods for producing the compositions from cacao beans, in particular unfermented cacao beans. The disclosed compositions have high concentrations of epicatechin, procyanidin B2 and theobromine and demonstrate high bioavailability in in vitro tests. These results suggest that the compositions developed are potentially useful for producing functional foods.

The application belongs to the technical field of natural fruit active substance detection, and discloses a method for simultaneously detecting the contents of multiple active components in green fruits based on HPLC, wherein the active components include seven main active components in green fruits, i.e., pyrogallol, gallic acid, epicatechin, ethyl gallate, p-coumaric acid, epigallocatechin and ellagic acid. The detection method established by the application is good and the instrument is precise, and the method has the technical advantages of good selectivity, high sensitivity and accuracy and reliability for detecting the contents of the main active components in green fruits, thereby providing a new analysis method and reliable basis for quality analysis and quality control of green fruits.

The invention belongs to the technical field of plant disease prevention and control, and particularly relates to application of epicatechin and protocatechuic acid in prevention and control of citrus huanglongbing. Through systematic experimental research and application verification, it is found that epicatechin and protocatechuic acid can remarkably inhibit the number of citrus huanglongbing pathogenic bacteria, have a good synergistic effect, can remarkably improve the overall health condition of citrus trees and have excellent application prospects and popularization value. As epicatechin and protocatechuic acid have the characteristics of being natural, environment-friendly and efficient, the epicatechin and protocatechuic acid show important application potential in the field of prevention and treatment of the citrus huanglongbing, and are expected to become novel key tools in the field. The discovery not only provides a new thought for green prevention and control of the citrus huanglongbing, but also provides powerful technical support for sustainable development of the global citrus industry, and is of great significance for guaranteeing stable development of the industry.

The invention discloses a preparation method and application of hempseed (-)-epicatechin-3-O-gallate, and belongs to the technical field of biology. According to the preparation method disclosed by the invention, the non-ester catechin and the ester-rich catechin are separated by adopting the mixed solvents of the ethanol, the methanol and the isopropanol, so that the extraction rate of the (-)-epicatechin-3-O-gallate can be improved (97% or above). Meanwhile, recrystallization is carried out by adopting organic solvents of methanol, normal propyl alcohol and isopropyl alcohol after extraction, so that the purity of the hempseed (-)-epicatechin-3-O-gallate can be improved (98% or above). The extracted epicatechin-3-O-gallate (ECG) is a natural antioxidant, has good biological activity, and has a good treatment effect on prevention and treatment of the hyperuricemia nephropathy.

The invention discloses an active composition for treating atopic dermatitis and application thereof, and belongs to the technical field of biological medicine. The (+)-catechin hydrate, the epicatechin, the chlorogenic acid and the amyrin in a specific proportion are compounded to serve as active ingredients, the multi-target and multi-channel synergistic interaction effect is achieved, and the effect of remarkably treating atopic dermatitis is achieved; besides, the components of the carrier are optimized, the stability, skin permeability and slow release performance of the active components are remarkably improved, percutaneous penetration and target site accumulation of the active components are enhanced, and therefore the local drug concentration and the curative effect durability are improved; the composition has the advantages that the composition can be rapidly formed at low temperature, the biological activity of the active ingredients is better, the composition can be processed and prepared into various local external dosage forms such as gel, cream, dressing and spray according to clinical requirements, the application is flexible, the adverse reaction possibly caused by long-term use of traditional hormone or chemical synthetic drugs is avoided, and the composition has a good industrial conversion prospect.

The invention discloses a quality detection method of a traditional Chinese medicine compound fester-clearing and ulcer-healing decoction. Chemical components in six medicinal materials including coptis chinensis, frankincense, sanguisorba officinalis, indigo naturalis, catechu and dragon's blood in the fester-clearing and ulcer-healing decoction are qualitatively analyzed by using a UPLC-Q-Exactive Orbitrap-MS / MS analysis method; and determining the contents of epicatechin, catechin, rutin, coptisine and berberine in the fester-clearing and ulcer-healing decoction by using a high performance liquid chromatography method. The UPLC-Q-Exactive Orbitrap-MS / MS analysis method has the advantages of high resolution, good quality precision, strong qualitative ability and the like, and can be used for widely and accurately detecting the chemical components in the complex traditional Chinese medicine compound; high performance liquid chromatography can provide detection results with high accuracy, high sensitivity and good repeatability, operation is simple and easy to implement, methodology verification is passed, the linear relation is good, precision, stability, repeatability, the recovery rate and the like all meet regulations, and the combination of qualitative analysis and quantitative analysis lays a foundation for establishment of the quality standard of the fester-clearing and ulcer-healing decoction.

The present application relates to the field of preparation of starch nanoparticles, and specifically provides a kind of epicatechin loaded pepper starch nanoparticles and its preparation method.The preparation method is to use pepper starch to load epicatechin, and through comparative experiment, it is proved that the specific process combination (debranching, stirring, homogenization) of the present application is crucial to realize high encapsulation rate.The finally prepared pepper starch nanoparticles can significantly improve the encapsulation rate of epicatechin.This means higher loading efficiency and lower waste of active ingredients, with significant industrial cost advantage.

The invention relates to the technical field of pharmaceutical biology, in particular to application of (-)-epicatechin to inhibition of toxicity caused by MEZ. The toxicity caused by the MEZ is developmental toxicity or hepatotoxicity. The developmental toxicity comprises the following symptoms: embryo malformation, embryo apoptosis and developmental morphology abnormality. The hepatotoxicity comprises liver development delaying, cholesterol and lipid metabolism disorder, liver injury and liver cell apoptosis. According to the research, the effect of the L-epicatechin on inhibiting MEZ-induced developmental toxicity and hepatotoxicity is found, embryo injury and liver injury caused by MEZ can be effectively relieved, and the pathogenesis of the MEZ-induced developmental toxicity and hepatotoxicity can be further understood.

The invention relates to a composition comprising a mixture of catechin and / or epicatechin, safranal and crocetin in aglycone and / or glycosylated form. Such a composition can be used as a nutritional product or medicament and has a synergistic action for improving cognitive functions and / or executive functions in humans or animals.

Methods steps for the synthesis of (−)-epicatechin are provided herein. The synthetic methods are provided in two phases: (1) synthesis of epoxide intermediates and (2) synthesis of (−)-epicatechin.

The invention provides an asparagus microspore induction culture medium and a culture method, and relates to the technical field of crop cultivation. The asparagus microspore induction culture medium comprises the following components, 50 to 1500 mg / L of KNO3, 300 to 340 mg / L of NH4H2PO4, 155 to 195 mg / L of CaCl2. 2H2O, 18 to 224 mg / L of MgSO4. 7H2O, 60 to 700 mg / L of K2SO4, 7.3 to 9.5 mg / L of MnSO4. 4H2O, 2.0 to 4.0 mg / L of ZnSO47H2O, 2.0 to 4.0 mg / L of H3BO3, 0.5 to 1.0 mg / L of KI, 2.0 to 2.4 mg / L of acetylcholine, 20 to 30 mg / L of spermidine, 5.0 to 6.0 mg / L of proline, 1.5 to 2.5 mg / L of glycine, 20 to 240 mg / L of glutathione, 80 to 100 mg / L of inositol, 2.4 to 3.6 mg / L of acetosyringone-220, 2 to 4 mg / L of The addition of acetylcholine, spermidine, proline, glutathione, acetosyringone, epicatechin, hydrocortisone and other additives can promote the induction of the microspore callus.

The invention discloses application of hempseed (-)-epicatechin-3-O-gallate, and belongs to the technical field of biology. According to the invention, the EPI (-)-epicatechin-3-O-gallate extracted from the EPI is applied to the diabetic kidney diseases, so that the EPI can significantly reduce blood sugar and blood fat levels, improve pathological changes of the kidney of diabetic mice, delay senescence and fibrosis of renal tubular epithelial cells of the diabetic mice, improve the drug efficacy of DKD (Dikyl Ketene Dimer), and can be used for treating the diabetic kidney diseases. And reliable treatment targets and theoretical basis are provided for treating DKD by natural medicine monomer components.