72 results about "L-Epicatechin" patented technology

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

The present invention provides a method for the preparation of a myceliated cacao bean or other agricultural product. This method includes providing cacao beans or other agricultural substrate, optionally hydrating the cacao beans or other agricultural product, and optionally pasteurizing or sterilizing the cacao beans or other agricultural substrate to provide prepared cacao beans or other agricultural substrate, and a step of inoculating the prepared cacao beans or other agricultural substrate with a prepared fungal component and culturing the inoculated cacao beans or other agricultural substrate to prepare the myceliated product. The methods of the instant invention result in prepared cacao beans or other agricultural substrate having reduced levels of undesirable taste components, such as theobromine, catechin, epicatechin, gallic acid equivalents, and / or 2-methoxy-3-isopropylpyrazine, and increased levels of myceliation products, such as fungal β-glucans, chitin, proteins, glycoproteins, pyrazines and polysaccharides, relative to starting cacao beans or other agricultural substrate.

The invention relates to a preparation method of an epicatechin monomer. The preparation method comprises the following steps of: (1) adding tannase to a solution containing large-leaf green tea catechin extract, carrying out static enzymolysis at 30-35 DEG C, intermittently stirring, and centrifugally filtering to obtain an enzymolysis solution; (2) introducing the enzymolysis solution into a chromatographic column loaded with adsorptive resin, carrying out gradient elution with ethanol elution agent, collecting eluate, concentrating eluate to 16-18 Baume degree to obtain concentrate, standing the concentrate at 0-4 DEG C for 48-72h, adding a little of epicatechin monomer, crystallizing and recrystallizing, centrifuging and drying to obtain the epicatechinmonomer. According to the method, epicatechin gallate is subjected to enzymolysis by using tannase, so that the method has good transformation effect, high transformation rate and high product quality, short preparation period, and low cost, and is suitable for scaled production.

The present invention pertains to the enhanced activity of (+) epicatechin over (−) epicatechin. The present invention is related to novel analogs of (+) epicatechin of the formula (I), which enhances the pharmacokinetics and therefore the pharmacodynamics of (+) epicatechin. The present invention is related to analogs of (+) epicatechin of the formula (I). The general structure of the analogs of the present invention may be represented by Formula (I): Formula (I) wherein A and B are independently OR1 and C and D are independently OH; wherein R1 is independently C1 to C10 lower straight or branched chain acyclic or cyclic alkyl, or is selected from the group comprising, hydroxy butyric acid, dichloroacetic acid; phenyl butyric acid; valproic acid.

The present invention discloses a novel, stable and synergistic combination of epicatechin with anti-cancer compounds. The present invention also discloses a composition comprising the novel combination of epicatechin with anti-cancer compounds along with other pharmaceutically acceptable excipients.

The present invention provides synthetic processes for preparing racemic and / or optically pure epicatechin, epigallocatechin and related polyphenols as such or as their variously functionalized derivatives. A principle objective of the disclosure is to provide a new and useful method of synthesis to obtain polyphenols in isomerically pure and / or racemic forms.

The present invention relates generally to catechin and epicatechin conjugates of formula (I). For example, a chemical synthesis process for the preparation of catechin and epi-catechin compounds, in particular of catechin and epi-catechin conjugates is disclosed. A further aspect of the invention pertains to new catechin and epi-catechin conjugate compounds.

The present invention relates to an improved process for the separation of gallated epicatechins (EGCG & ECG) from green tea extract or green tea dust. The present invention specifically relates to animproved process for the separation of gallated epicatechins (EGCG & ECG) from green tea extract or green tea dust by forming hydrated gallated epicatechins (EGCG & ECG)-isonicotinic acid complex using isonicotinic acid as acid, organic solvents for liberating gallated epicatechins. The present invention more specifically relates to the process for the separation of gallated epicatechins (EGCG &ECG) from green tea extract or green tea dust, wherein processes comprises steps of dissolving, concentrating by centrifugation, adding, precipitating, filtering, slurrying, removing supernatant liquid, washing, extracting and freeze drying. The present invention also relates to hydrated gallated epicatechins (EGCG &ECG)-isonicotinic acid complex.

The invention discloses a persimmon proanthocyanidins precursor transmembrane transport gene DkMATE7 and an application thereof, and belongs to the technical field of plant genetic engineering. A differential expression gene DkMATE7 gene is screened and obtained for the first time through a transcriptome database, and meanwhile, it is found for the first time that DkMATE7 protein has a proanthocyanidins transport function. In-vivo complementary experimental analysis of Escherichia coli further proves that the DkMATE7 gene promotes preference transmembrane transport of persimmon procyanidine precursor catechin, epicatechin and epicatechin gallic acid. A scientific basis is provided for deeply researching the transport and accumulation of the procyanidins in the sweet persimmons, and a new gene resource is also provided for genetic improvement.

The invention discloses a molecular marker site linked with epicatechin (epicatechin, EC) content of camellia sinensis trees and an application of the molecular marker site. For the first time, the inventor of the invention finds that an SNP molecular marker site pertinent to the epicatechin content of the camellia sinensis trees on a camellia sinensis tree genome Scaffold2233: 468642, and the genotype is significantly correlated with the epicatechin content. The relevance and significance verification result indicates that compared with GG genotype samples and GA genotype samples, the epicatechin content in tea soup dry substances corresponding to AA genotype samples has significant differences. Judgment is performed through statistics, when the genotype is double mutation AA, the epicatechin content in dry substances in the camellia sinensis trees is higher than that of the normal average level obtained from wild type GG or single mutation genotype GA by large probability. The detection method for detecting the site is further established, can be used for evaluating the epicatechin content of the camellia sinensis trees to be further used for screening and molecular breeding of high-EC (epicatechin) camellia sinensis tree resources, and has great research value.