Method for efficiently and visually discriminating authenticity of Tetrastigma hemsleyanum Diels et Gilg by using DNA mimic enzyme
A cloverleaf, simulating enzyme technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of no verification, time-consuming, etc., avoid gel electrophoresis analysis, and the method is simple , good stability
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[0026] Such as figure 1 As shown, the method for efficiently and visually identifying the authenticity of Clover clover provided by this embodiment includes the following steps:
[0027]Step A, DNA extraction of the genuine and counterfeit Clover: the plant DNA extraction CTAB method is used to extract the DNA of the genuine Clover and the fake Clover; wherein the genuine Clover comes from Zhejiang, Guangxi, Hunan and Jiangxi, etc. The fake Clover is selected from Tuyuaner. Specific steps are as follows:
[0028] (1) 10mL CTAB (cetyltrimethylammonium bromide) separation buffer (CTAB separation buffer is: 2% cetyltrimethylammonium bromide, 20mM ethylenediaminetetraacetic acid disodium salt , 100mM tris-hydrochloric acid (PH=8.0), 1.4M sodium chloride, 0.2% mercaptoethanol) into a 50mL centrifuge tube, and placed in a 60°C water bath to preheat.
[0029] (2) Take 2.0ɡ leaves, place them in a pre-cooled ceramic mortar, pour liquid nitrogen into them, and grind the leaves quick...
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