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Ethanol extract ethyl acetate extractive fraction of Tetrastigma hemsleyanum leaf and preparing method and application thereof

A technology of ethyl acetate extraction and ethanol extraction, which is applied in applications, food preparation, skin care preparations, etc., to achieve the effect of inhibiting bacterial growth

Inactive Publication Date: 2015-11-18
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to extract the best antibacterial clover stock solution through the extract is not disclosed in the patent application specification.

Method used

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  • Ethanol extract ethyl acetate extractive fraction of Tetrastigma hemsleyanum leaf and preparing method and application thereof
  • Ethanol extract ethyl acetate extractive fraction of Tetrastigma hemsleyanum leaf and preparing method and application thereof
  • Ethanol extract ethyl acetate extractive fraction of Tetrastigma hemsleyanum leaf and preparing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Get the leaf pulverization of dry Cloverleaf to obtain dry powder, use volume percentage 95% ethanol aqueous solution as solvent extraction 90min, the consumption of ethanol aqueous solution is 20mL / g of dry powder quality, filter, and filtrate is dried to obtain the total amount of Cloverleaf. Extract;

[0025] (2) Suspending the total extract of A. trifoliate with water at 1:1, extracting with ethyl acetate, and evaporating the extract to dryness to obtain the ethyl acetate extraction part of the ethanol extract of A. trifoliate;

[0026] (3) The ethanol extract ethyl acetate extraction part of the leaf of Cloverleaf is dissolved in the water containing 2% DMSO by volume percentage with the concentration of 16mg / mL, after the filter membrane filtration of 0.45 μm is sterilized, makes antibacterial medicinal liquid, Each drug solution was stored at 4°C for later use.

[0027] (4) Detect the minimum inhibitory concentration (MIC) of each liquid medicine on bacteria...

Embodiment 2

[0033] (1) Get the leaf pulverization of dry Cloverleaf to obtain dry powder, use volume percentage 95% ethanol aqueous solution as solvent extraction 90min, the consumption of ethanol aqueous solution is 20mL / g of dry powder quality, filter, and filtrate is dried to obtain the total amount of Cloverleaf. Extract;

[0034](2) Suspending the total extract of A. trifoliate with water at 1:1, extracting with ethyl acetate, and evaporating the extract to dryness to obtain the ethyl acetate extraction part of the ethanol extract of A. trifoliate;

[0035] (3) The ethanol extract ethyl acetate extraction part of the leaf of Cloverleaf is dissolved in the water containing 2% ethanol by volume percentage with the concentration of 16mg / mL, after the filter membrane filtration of 0.45 μm is sterilized, makes antibacterial medicinal liquid, Each drug solution was stored at 4°C for later use.

[0036] (4) Detect the minimum inhibitory concentration (MIC) of each liquid medicine on bacter...

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Abstract

The invention discloses an ethanol extract ethyl acetate extractive fraction of tetrastigma hemsleyanum leaf and a preparing method and application thereof. The method includes: 1, crushing dried leaves of tetrastigma hemsleyanum to obtain dry powder, extracting with ethanol water, performing filtering, drying filtrate to obtain total extract of the tetrastigma hemsleyanum leaf; 2, mix-suspending the total extract of the tetrastigma hemsleyanum leaf and water, extracting with ethyl acetate to obtain ethyl acetate extract, and steam-drying the ethyl acetate extract to obtain the ethanol extract ethyl acetate extractive fraction of tetrastigma hemsleyanum. The ethanol extract ethyl acetate extractive fraction of the tetrastigma hemsleyanum leaf prepared by the preparing method is applicable to the preparation of antibacterial products, is effective in inhibiting germ growth and killing germs, applicable to drugs, cosmetics, health foods, foods and applicable to bacterial inhibition for the human, animals and the aquaculture process, and has a promising application prospect.

Description

technical field [0001] The present invention relates to the technical field of trifoliate leaf extract, in particular to an ethyl acetate extraction part of an ethanol extract of trifoliate green leaf and its preparation method and application. Background technique [0002] The traditional Chinese medicine clover (Tetrastigmahemsleyanum Dielset Gilg) is a vine of the family Vitis genus. It grows in damp hillsides, valleys or under forests beside streams. It is mainly distributed in southwest my country and Zhejiang, Anhui, Jiangxi, Fujian and Guangxi. And other provinces. Clover is mainly used as root, and the whole plant is also used as medicine, but there is no special description. At present, Clover sold in pharmacies and commercially available Chinese herbal medicine powder are all from the root of Clover. Qing also mostly refers to the root part of Clover. The roots of Clover have the effects of improving immunity, anti-virus, and enhancing liver function, especially f...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P31/04A61K8/97A61Q19/00A23L1/30
Inventor 许海顺吴学谦俞盈熊科辉么春艳徐娟
Owner ZHEJIANG FORESTRY UNIVERSITY
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