Method for efficiently and rapidly propagating tetrastigma hemsleyanum Diels et Gilg test-tube plantlet

A technology of clover and test-tube seedlings, which is applied in the field of plant cultivation, can solve the problems of low cultivation efficiency and achieve the effects of neat germination, low variation rate, and high transplanting survival rate

Active Publication Date: 2015-12-23
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for efficiently and rapidly propag

Method used

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  • Method for efficiently and rapidly propagating tetrastigma hemsleyanum Diels et Gilg test-tube plantlet
  • Method for efficiently and rapidly propagating tetrastigma hemsleyanum Diels et Gilg test-tube plantlet
  • Method for efficiently and rapidly propagating tetrastigma hemsleyanum Diels et Gilg test-tube plantlet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Cut the stem section of Clover, remove the leaves, cut into small sections of about 10cm, wash with household detergent for 10 minutes, and then rinse with running water for 10 minutes, and bring the cleaned branches into the ultra-clean workbench, 75% volume Soak in fractional alcohol for 45s, then soak in mercuric chloride aqueous solution for 6min; rinse with sterile water 3 to 5 times.

[0030] (2) Cut the aseptic stem section of Clover clover obtained in step (1) into small sections with 1 to 2 axillary buds and inoculate them in solid medium, at 22 to 25°C, about 700 lux of illumination, and 12 hours of light per hour Under the condition of 45 days, the axillary buds germinate after culturing for about 45 days. When the buds grow to 1-2 cm, take the axillary buds for later use; wherein, the composition of the solid medium is: 6-benzylaminopurine 1.0mg / L+sucrose 30g / L+agar 6g / L+potato juice 60g / L+activated carbon 0.5g / L+MS medium+pH5.8.

[0031] (3) Place the ...

Embodiment 2

[0035] Substantially the same as in Example 1, the axillary buds germinate in step (2), step (3), and step (4), and the three stages of subculture proliferation cultivation and strong seedling cultivation are respectively screened with the medium combinations listed in Table 1 to optimize Optimal medium combination formula. Table 1 explains as follows:

[0036]① The optimization of the medium formula is mainly to design different basic medium, hormone concentration, etc. to optimize the design of the medium. In this example, it is designed as a solid medium. The agar concentration in each formula is 6g / L, and the sucrose is 30g / L, the culture environment is a culture room with a temperature of 25°C, a light intensity of 1000-2000 lux, and a light time of 12h / d. ②Aiming at production, the culture medium formula is designed in different stages of cultivation. In the stage of germination of the axillary buds of C. trifoliata, the indicators such as the speed of germination, gro...

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Abstract

The invention discloses a method for efficiently and rapidly propagating a tetrastigma hemsleyanum Diels et Gilg test-tube plantlet. According to the method, an annual semi-lignified stem section of tetrastigma hemsleyanum Diels et Gilg is sterilized, rinsed and sheared, and small sections with 1-2 axillary buds are obtained and inoculated to a solid medium to be cultured; after the axillary buds germinate, the axillary buds are inoculated to a proliferation medium to be cultured and grafted once every 50-60 days until the required seedling quantity is reached, the axillary buds are inoculated to a rooting medium for rooting culture, and after 50-60 days, when the seedlings of the tetrastigma hemsleyanum Diels et Gilg grow 2-3 roots, acclimatizing and transplanting can be performed. Compared with the prior art, the method can be directly used for industrial and large-scale seedling production and has the advantages of little pollution, high proliferation speed, low aberration rate, orderly and robust seedlings, low production cost, convenience in transportation, high survival rate of transplanting and the like.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for efficiently and rapidly propagating test-tube seedlings of A. trifoliate. Background technique [0002] Clover, scientific name: Tetrastigmahemsleyanum DielsetGilg, also known as golden thread hanging gourd, snake monkshood, stone mouse, belongs to Rhamnaceae, Vitaceae, and Tetrastigmahemsleyanum Dielset Gilg is a herbaceous vine. It is a unique medicinal plant in my country, widely distributed in the provinces south of the Yangtze River, mainly in Zhejiang, Jiangxi, Fujian, Hunan, Hubei, Guangdong, Guangxi, Guizhou, Chongqing, Sichuan, Tibet, Yunnan, Hainan and Taiwan. In thickets under forests on hillsides or in crevices of rocks under forests in valleys and streams, growing at an altitude of 300-1300m [0003] The whole herb of Clover can be used as medicine, and the medicinal effect of underground roots and fruits is the best. It is flat in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 史俊贾君席刚俊汪善锋徐超杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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