Method for efficiently and rapidly propagating tetrastigma hemsleyanum Diels et Gilg test-tube plantlet
A technology of clover and test-tube seedlings, which is applied in the field of plant cultivation, can solve the problems of low cultivation efficiency and achieve the effects of neat germination, low variation rate, and high transplanting survival rate
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Embodiment 1
[0029] (1) Cut the stem section of Clover, remove the leaves, cut into small sections of about 10cm, wash with household detergent for 10 minutes, and then rinse with running water for 10 minutes, and bring the cleaned branches into the ultra-clean workbench, 75% volume Soak in fractional alcohol for 45s, then soak in mercuric chloride aqueous solution for 6min; rinse with sterile water 3 to 5 times.
[0030] (2) Cut the aseptic stem section of Clover clover obtained in step (1) into small sections with 1 to 2 axillary buds and inoculate them in solid medium, at 22 to 25°C, about 700 lux of illumination, and 12 hours of light per hour Under the condition of 45 days, the axillary buds germinate after culturing for about 45 days. When the buds grow to 1-2 cm, take the axillary buds for later use; wherein, the composition of the solid medium is: 6-benzylaminopurine 1.0mg / L+sucrose 30g / L+agar 6g / L+potato juice 60g / L+activated carbon 0.5g / L+MS medium+pH5.8.
[0031] (3) Place the ...
Embodiment 2
[0035] Substantially the same as in Example 1, the axillary buds germinate in step (2), step (3), and step (4), and the three stages of subculture proliferation cultivation and strong seedling cultivation are respectively screened with the medium combinations listed in Table 1 to optimize Optimal medium combination formula. Table 1 explains as follows:
[0036]① The optimization of the medium formula is mainly to design different basic medium, hormone concentration, etc. to optimize the design of the medium. In this example, it is designed as a solid medium. The agar concentration in each formula is 6g / L, and the sucrose is 30g / L, the culture environment is a culture room with a temperature of 25°C, a light intensity of 1000-2000 lux, and a light time of 12h / d. ②Aiming at production, the culture medium formula is designed in different stages of cultivation. In the stage of germination of the axillary buds of C. trifoliata, the indicators such as the speed of germination, gro...
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