Primer, probe and kit for detecting microorganism in urogenital canal and use method of kit

Abstract

The invention discloses a primer, a probe and a kit for detecting the microorganism in a urogenital canal and a use method of the kit. By the primer, the probe and the kit for detecting the microorganism in the urogenital canal and the use method of the kit, the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus can be detected simultaneously, the extraction of the nucleic acid is only conducted and only one step of the PCR detection reaction is conducted through the real-time fluorescence PCR amplification technique, the use method of the kit is simple and rapid, the specificity is good, the pathogene microorganisms of the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus are not detected, the problem of false positive result is not easy to cause, thesensitivity is as high as 400 copies / ml, the detection range is 400 copies / ml to 4.00E+09 copies / ml, and the detection range is wide.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a primer, a probe, a kit and a method for using the kit for the detection of microorganisms in the urogenital tract. Background technique [0002] The study of urogenital microorganisms has important clinical and scientific value. There are many kinds of microorganisms in the genitourinary tract, and the common ones are Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU) and Neisseria gonorrhoeae (NG). [0003] Chlamydia trachomatis is a prokaryotic microorganism with a unique developmental cycle and strict intracellular parasitism. It is a special microorganism between viruses, bacteria and Rickettsia. Chlamydia, Chlamydia, and Chlamydia. Chlamydia trachomatis is the most common genitourinary tract infectious microorganism at home and abroad, and ranks third among STDs in genital tract Chlamydia infection in my country, and its incidence rate is increasing year b...

AI Technical Summary

Problems solved by technology

[0007] At present, there are some kit products based on real-time fluorescent quantitative PCR technology to detect Chlamydia trachomatis, Ureaplasma urealyticum or Neisseria gonorrhoeae DNA, but there are generally problems of poor specificity and low sensitivity, and most of them are for Chlamydia trachomatis, Ureaplasma urealyticum A single detection kit for pathogens or Neisseria gonorrhoeae is particularly poor for the detection of a mixture of pathogenic microorganisms, and the processing process is cumbersome

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