Clopidol colloidal gold chromatography detection test strip or card

A technology of pyridine and colloidal gold, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of high price, complicated operation process, and application limitation of ELISA method, and achieve strong timeliness, high sensitivity, and easy promotion Effect

Inactive Publication Date: 2013-04-03
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ELISA method requires a supporting microplate reader and supporting reagents, and the operation process is still relatively complicated. Moreover, the country is currently in the research and development stage, and imported foreign products are expensive, so the application of the ELISA method is greatly restricted.

Method used

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  • Clopidol colloidal gold chromatography detection test strip or card
  • Clopidol colloidal gold chromatography detection test strip or card
  • Clopidol colloidal gold chromatography detection test strip or card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of chlorhydrin antigen, antibody

[0030] 1.1 Modification and identification of the clohydroxypyridine hapten

[0031] The nucleophilic substitution reaction introduces an ester bond (-COO- group), and then under the action of alkali and acid, a carboxyl-containing cloxyridine hapten is synthesized.

[0032] Take 5mmoL CLOP and dissolve it in 15ml 0.5mol / L NaOH solution. After it is completely dissolved, distill it under reduced pressure to remove the water. Esters were reacted at a constant temperature of 60°C for 3 hours, cooled to room temperature, 150mL of distilled water was added to the reaction liquid, cooled overnight in a refrigerator at 4°C, filtered with suction → washed with water → filtered with suction to obtain CLOP derivatives containing ester bonds. Then add 20mL of distilled water, heat to 85°C, add dropwise 1mol / L NaOH solution to dissolve all the solids, cool to room temperature, then add dropwise 1mol / L HCI to make th...

Embodiment 2

[0039] Embodiment 2, the preparation of CLOP colloidal gold immune test strip and test paper card

[0040] 2.1 Colloidal gold chromatography detection test strip and test card detection reaction principle

[0041] When the colloidal gold chromatography detection test strip or the test paper card test end is added to the sample solution to be tested, the solution to be tested will drive the sample to be tested and the gold-labeled antibody in the gold-labeled binding pad to diffuse to the coating film through siphon action, and finally Infiltrate absorbent filter paper at the handle end. During the diffusion process, the analyte can be combined with the gold-labeled antibody, thereby blocking the antigen binding point of the analyte on the gold-labeled antibody, preventing the gold-labeled antibody from combining with the detection line coupled to the carrier protein antigen on the coating membrane, and the detection line It cannot develop color, but the goat or rabbit anti-mo...

Embodiment 3

[0049] Embodiment 3, the performance of the chlorhydrin colloidal gold immune test strip and the test paper card of the present invention

[0050] 3.1 Sensitivity test of chlorhydrin colloidal gold immune test strip and test paper card of the present invention

[0051] Add the CLOP standard with the concentration of clopyridine mother solution (1mg / mL) diluted to 0, 2.0, 4.0, 8.0, 16.0, 32.0 μg / L and 64 μg / L respectively to CLOP-test strip and test paper card (CLOP-Strip ) on the sample pad, let stand to react for 10 minutes, and read the relative optical density value (G / D×A-ROD(pixel) of the T-line scanning area optical density with a strip reader, where G is a graph indicating a chart, and D is Density means optical density, A means area means scanning area, D×A means optical density of scanning area, ROD means relative optical density means relative optical density value, pixel means image speed at different distances), standard products with different concentrations and b...

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PUM

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Abstract

The invention discloses a clopidol colloidal gold chromatography detection test strip or card which comprises a lining board, a sample pad, a gold mark combined pad, a nitrocellulose membrane and a glass water-absorbing pad, wherein the sample pad, the gold mark combined pad, the nitrocellulose membrane and the glass water-absorbing pad are sequentially connected on the lining board; the gold mark combined pad is an anti-clopidol monoclonal antibody or a polyclonal antibody glass fiber cotton which is absorbed with a colloidal gold mark; and an invisible detection line printed by using a clopidol-carrier protein conjugate and an invisible comparison line printed by using goat anti-mouse IgG are positioned on the nitrocellulose membrane. The clopidol colloidal gold chromatography detection test strip and card disclosed by the invention has the advantages of high specificity, high sensitivity, easiness, convenience, high speed, high timeliness, vivid, visual and accurate result display, cost saving, wide application range and convenience for popularization.

Description

technical field [0001] The invention relates to a device for rapidly detecting a small amount of cloxazine, in particular to a colloidal gold chromatography detection test strip or card for the analysis of cloxazine residues in feed and animal-derived food. Background technique [0002] Chloroxypyridine (CLOP), the trade name is gram phenol, the chemical name is 3,5-dichloro-2,6-dimethyl-4-hydroxypyridine, it is a feed drug for preventing coccidiosis in livestock and rabbits Additives, long-term use or non-compliance with prescribed medication will cause chlorhydrin residues in animals and tissues, and have certain teratogenicity and embryotoxicity. Now, the European Union, the United States, Canada, Japan, China, and Taiwan of China have all stipulated the maximum limit of chlorhydrin residues in various animal tissues. my country has stipulated that the residue limit of chlorhydrin in chicken is 0.01mg / kg. [0003] The detection methods of chlorhydrin in tissues mainly a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
Inventor 张海棠范国英王自良姜金庆王淑云杨雪峰丁涵李艺
Owner HENAN INST OF SCI & TECH
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