Test paper for detecting pig breeding disorder virus epidemic pathogen

A technology for pig reproduction and infectious diseases, which is applied in the field of test strips for detecting the pathogens of porcine reproductive disorder virus infectious diseases, can solve the problems of inconvenient use by grassroots personnel, and achieve false negative and false positive misjudgments, reduced investment, and high efficiency. Effects of Specificity and Sensitivity

Inactive Publication Date: 2009-01-07
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the dot immunogold diafiltration method reported at present only detects a kind of swine disease pathogen once, even so, also need to add 2 times of reagents and wash 2 times at least and just can obtain result; A variety of kits and more than a dozen operating steps, it is still inconvenient for non-professional grassroots personnel to use
[0005] Patent document CN101216488A discloses a test strip for detecting porcine reproductive and respiratory syndrome antibody and its preparation method, which is to diagnose by detecting PRRSV antibody instead of directly detecting the presence of the virus, and can only indirectly diagnose three kinds of One of the major porcine reproductive disorder viruses (PRRSV), with significant limitations

Method used

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  • Test paper for detecting pig breeding disorder virus epidemic pathogen
  • Test paper for detecting pig breeding disorder virus epidemic pathogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: A kind of test strip that simultaneously detects three kinds of porcine reproductive disorder virus infectious disease pathogens, see figure 1 and figure 2 , in the figure 1 is the support layer made of plastic thin strips, 2 is the sample adsorption layer made of glass wool, 3 is the anti-PRRSV (code R), anti-PPV (code P) and anti-PRRSV (code P) with colloidal gold labels attached. The glass wool fiber layer of JEV (code name J) monoclonal antibody (Wi), 4 is the nitrocellulose membrane, and 5 is the water-absorbing layer made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted on the support in turn. On layer 1, the fibers at the junction where they are spliced ​​with each other interpenetrate with each other. On the nitrocellulose membrane 4, the detection blots 6 (R+P+J) of the three viruses were marked with anti-PRRSV, anti-PPV, and anti-JEV polyclonal antibody IgG solutions (Xi) respectively, and the detection blots 6...

Embodiment 2

[0042] Example 2: A test strip for simultaneous detection of three porcine reproductive disorder virus infectious disease pathogens, its structure and preparation method are basically the same as in Example 1, the difference is: the gold-labeled antibody fiber layer made of nylon fibers Colloidal gold-labeled anti-PRRSV, anti-PPV and anti-JEV gold-labeled polyclonal antibodies (Xi) (R+P+J) are attached to 3; on nitrocellulose membrane 4, anti-PRRSV, anti-PPV and anti-JEV Monoclonal antibody IgG solution (Wi) sprays three detection blots (R+P+J), and goat (or rabbit) anti-pig IgG solution sprays a control blot C, and the arrangement of the detection blots and control blots is " / / / / ","king"," ", "...", "∷". Others, including test sample preparation, operation method and result judgment, are the same as in Example 1 and will not be repeated.

Embodiment 3

[0043] Example 3: A test strip for simultaneously detecting two kinds of porcine reproductive disorder virus infectious disease pathogens, its structure and preparation method are basically the same as in Example 1, the difference is: the gold-labeled antibody fiber layer made of polyester fiber Only anti-PRRSV, anti-PPV gold-labeled monoclonal antibody (Wi) is attached on it; On the cellulose film layer 4 that polyvinylidene fluoride is made, respectively anti-PRRSV, anti-PPV polyclonal antibody IgG solution (Xi) Mark the two detection blots, and print the control blot with goat (or rabbit) anti-mouse IgG solution, and the arrangement of the detection blot and the control blot is " ", " / / / ", " ", "±", "...", " ", "∵". Others including test sample preparation, operation method and result judgment are the same as in Example 1.

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Abstract

The invention discloses a test strip which is used for detecting porcine reproductive difficulty virus infectious disease pathogen, comprising a non-hygroscopic support layer and an adsorption layer which adheres to the support layer. The adsorption layer consists of a sample adsorption fiber layer, a gold antibody fiber layer, a cellulose film layer and a hygroscopic layer, and the four layers are spliced sequentially. The cellulose film layer has a contrast blot which contains anti-porcine/mouse IgG and at least one detect blot which contains porcine reproductive difficulty virus antibody that is one of anti-PRRSV, PPV and JEV monoclonal antibodies/polyclonal antibodies; the gold antibody fiber layer is adhered with gold polyclonal antibodies/monoclonal antibodies of anti-porcine reproductive difficulty virus corresponding to the virus antibody. When used for testing, the test strip has the advantages of strong specificity, high sensitivity and no need of additional device or reagent. The test strip can be operated by everyone and is suitable for fast identifying and diagnosing porcine reproductive difficulty virus infectious disease on site.

Description

technical field [0001] The invention relates to a device for detecting the pathogen of porcine viral infectious disease, in particular to a test strip for detecting the pathogen of porcine reproductive disorder viral infectious disease. technical background [0002] my country is a big producer and consumer of live pigs. With the number of large, medium and small-scale pig farms and breeding pig farms increasing year by year, pig reproductive disorders have become one of the most important diseases in large-scale pig farms in my country, with an average incidence of more than 30%, causing huge economic losses. losses, and seriously restrict the development of the pig industry. Porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV) and porcine Japanese encephalitis virus (JEV) are three important viruses that cause reproductive disorders in pigs. PRRSV causes porcine reproductive and respiratory syndrome (PRRS), which is mainly manifested as fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/545G01N33/544
Inventor 张改平肖治军李学伍邓瑞广杨艳艳杨继飞赵东王爱萍邢广旭柴书军刘庆堂
Owner HENAN ACAD OF AGRI SCI
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