Colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and preparation method

Abstract

The invention discloses a colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and a preparation method thereof. The test strip consists of a lining plate and a sample pad, a gold label bonding pad, a coating film and a water absorption pad engaged on the lining plate in turn, wherein on the coating film, a straight detection line T is coated by using carrier protein solution coupled with the sibutramine and the derivative thereof, and a straight contrast line C is coated by using goat-anti-mouse IgG solution. The method comprises the following steps of: preparing artificial conjugated antigens; preparing monoclonal antibodies of the sibutramine and the derivative thereof; preparing colloidal gold solution; and obtaining a colloidal gold marker and the gold label bonding pad for resisting the monoclonal antibodies of the sibutramine and the derivative thereof. The test strip has the advantages of strong specificity, high sensitivity, strong timeliness and vivid and accurate result display, and is simple, convenient and quick.

Description

technical field [0001] The invention relates to a colloidal gold chromatography test strip and a preparation method for rapidly detecting sibutramine and its derivatives, belonging to the technical field of detection of biological immune methods. Background technique [0002] Sibutramine is a weight-loss drug that acts on the central nervous system. It has the effects of excitement and appetite suppression. It may cause serious side effects such as increased blood pressure, increased heart rate, anorexia, insomnia, and abnormal liver function. Because of its low price and certain effect of suppressing eating and losing weight, it has been illegally added to weight-loss health care products by some unscrupulous manufacturers for profiteering. According to the survey, this ingredient is added to 98% of the weight loss products sold on the market. Sibutramine will increase the risk of heart disease and stroke for users. Its instructions also clearly list that patients with uns...

AI Technical Summary

Problems solved by technology

Sibutramine will increase the risk of heart disease and stroke for users. Its instructions also clearly list that patients with unsatisfactory hypertension control, as well as patients with coronary heart disease, heart failure, arrhythmia and stroke are contraindicated in taking the drug. If consumers take weight-loss health food containing this ingredient without knowing it, there may be serious consequences. Therefore, in order to ensure the safety of people's diet and medicine, we must intensify efforts to crack down on illegal additions

However, in recent years, the means of illegal production by criminals have become increasingly concealed, and the detection method of sibutramine is relatively complicated, requiring sophisticated analytical instruments and professional technicians, resulting in high costs for supervision and random inspection by regulatory authorities, but the results are not satisfactory.

For example, a patent with application number 200910030401.1: a colloidal gold chromatography test strip for rapid detection of sibutramine and its preparation method. The preparation method adopts a soaking process, which takes 2-3 hours for a long time and is slow to dry. Gold chromatography test strips are prepared based on colloidal gold-labeled polyclonal antibodies, which have low affinity, weak specificity, and are prone to crossover

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