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Phenobarbital fast detecting test paper bar

A technology for detecting phenobarbital and test strips, which is applied in the field of rapid detection test strips for phenobarbital, which can solve the problems of shortening the detection time, false detection, missed detection, etc., to ensure food safety, low cost, and not easy to false positive and the effect of false negatives

Inactive Publication Date: 2006-08-02
河南省动物免疫学重点实验室
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Problems solved by technology

The detection process is about 2 to 4 hours, which greatly shortens the detection time and reduces the detection cost. It can be used for both qualitative and quantitative detection, but the probability of false positive or false negative is relatively high.
Since ELISA is a highly sensitive detection method, different operators often have different results, so this method often causes artificial false detection and missed detection.
At present, the immunological detection method of phenobarbital is still blank in China, and urgently needs to be studied and solved

Method used

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  • Phenobarbital fast detecting test paper bar
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Examples

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Effect test

Embodiment 1

[0035] Embodiment 1. See Figure 1 and Figure 2. In the figure, 1 is the support layer, made of hard plastic strips, 2 is the test end adsorption fiber layer, made of glass fiber cotton, and 3 is the phenobarbital gold-labeled antibody fiber layer, according to the above specific implementation (3) The described preparation method is to prepare glass fiber cotton with anti-phenobarbital monoclonal antibody gold-labeled antibody, 4 is a cellulose membrane layer, this embodiment uses a nitrocellulose membrane, and 5 is a water-absorbing material layer at the handle end. It is made of filter paper; the layers numbered 2, 3, 4, and 5 are pasted and fixed on the plastic sheet 1 from left to right, and the fibers at the junction between each other cross and penetrate each other. On the cellulose membrane layer 4, 6 is the invisible detection band, coupled with the phenobarbital carrier protein solution is bovine serum albumin (BSA), blotted on the cellulose membrane to make the detection...

Embodiment 2

[0040] Example 2: The structure of the phenobarbital rapid detection test strip is basically the same as that of Example 1, except that: the support layer 1 is made of non-absorbent hard paper, and the test end adsorption fiber layer 2 is made of nylon membrane, benzene Barbiturate gold-labeled antibody fiber layer 3 is adsorbed with anti-phenobarbital polyclonal antibody, cellulose membrane layer 4 is made of pure cellulose membrane, and stealth control blotting band 7 is prepared on the cellulose membrane with goat anti-rabbit IgG antibody solution. to make.

[0041] Others including the preparation of the test sample, the operation method and the result determination are the same as in the first embodiment, and will not be repeated.

Embodiment 3

[0042] Example 3. The structure of the phenobarbital rapid detection test strip is basically the same as that of Example 1. The difference is that the test end adsorption fiber layer 2 uses a polyvinylidene fluoride (PVDF) film, and the cellulose film layer 4 uses carboxylate. Cellulose membrane, invisible detection blot strip 6 is coupled with phenobarbital carrier protein solution is chicken ovalbumin (OVA), invisible control blot strip 7 is prepared on cellulose membrane with rabbit anti-mouse IgG antibody solution .

[0043] Others including the preparation of the test sample, the operation method and the result determination are the same as in the first embodiment, and will not be repeated.

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Abstract

The present invention discloses a phenobarbital quick detection test paper strip. It contains bottom layer as supporting layer, intermediate layer as adsorption layer and protective layer fixed on the adsorption layer, Said adsorption layer successively includes test end adsorption fibre layer, adsorption gold-labelled amtibody fibre layer, cellulose membrane layer and water-absorbing material layer of handle end. Besides, said invention also provides the concrete structure on the cellulose membrane layer, said structure include linear detection blotting and linear reference blotting.

Description

[0001] 1. Technical Field: The present invention relates to a device for rapid detection of trace sedative drugs, in particular to a test strip for rapid detection of phenobarbital. 2. Technical background: [0002] Modern aquaculture provides humans with a rich variety of foods with high nutritional value such as meat, poultry, milk, eggs, etc., improves human dietary structure, improves human material life and health, and contributes to extending human life. . At the same time, modern scientific research has proved that the large-scale use of feed additives in the breeding industry, as well as the need for disease prevention and treatment, the large-scale use of antibiotics, hormones and other synthetic drugs, is promoting animal growth, reducing animal morbidity and mortality, and improving feed utilization. At the same time, it also caused drug residues and hormone residues in animal food. In addition, due to the lack of scientific knowledge and economic interests, the illegal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544G01N33/577G01N33/52
Inventor 张改平王自良邓瑞广杨艳艳李学伍王选年肖治军李青梅杨继飞柴书军刑广旭赵东李灵霞王建中
Owner 河南省动物免疫学重点实验室
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