299 results about "Immunologic Technique" patented technology

The present invention provides novel osmium-based electrochemical species for the detection of wide variety of analytes using immunological techniques. The present invention also provides diagnostic kits and test sensors supporting electrode structures that can be used with the osmium-based electrochemical species. The test sensor can be fabricated to support interdigitated arrays of electrodes that have been designed to provide amplification of the electrical signal amplification desired to analyze analytes that may be present at low concentrations.

The invention belongs to the field of immunological technique and discloses a method for lymphocyte subsets and a kit thereof. The method of lymphocyte subsets provided by the invention includes T lymphocyte subsets and B lymphocyte subsets. T lymphocyte subsets contain the following steps of: getting different fluorescence labeled antibodies, mixing with a sample to be detected, incubating, detecting by flow cytometry to obtain test data and analyzing the test data. B lymphocyte subsets contain the following steps of: getting different fluorescence labeled antibodies, mixing with a new aforementioned sample to be detected, incubating, detecting by flow cytometry to obtain test data and analyzing the test data. The method provided by the invention is used to realize a more comprehensive fine immunophenotyping of lymphocyte, has advantages of less sample needed to be detected, simple operation, short time needed and high precision, and can be widely applied in immunophenotyping of lymphocyte subpopulation.

The invention relates to a PDL1 monoclonal antibody and application thereof and belongs to the technical field of immunology. A separated human specific binding molecule provided by the invention comprises a) and b), wherein a) three light-chain CDRs comprise a light-chain CDR1, a light-chain CDR2 and a light-chain CDR3; b) three heavy-chain CDRs comprise a heavy-chain CDR1, a heavy-chain CDR2 anda heavy-chain CDR3; the separated human PDL1 specific binding molecule is a separated antibody or antigen binding fragment. The PDL1 monoclonal antibody provided by the invention can be used for effectively inhibiting partial tumor growth; a PD1 / PDL1 signal is blocked so that the proliferation of tumor antigen specific T cells can be accelerated and the effect of killing tumor cells is realized;a related PDL1 signal on the tumor cells is blocked so that the secretion of an infiltrated CD8<+>T cell IFN-gamma can be up-regulated.

A colloidal gold chromatographic test strip for rapidly testing lead ion belongs to the field of immunological technique. The test strip provided by the invention is composed of a liner plate and a sample pad, a colloidal-gold combination pad, a coating membrane and a water absorption pad which are successively connected on the liner plate. The colloidal-gold combination pad is glass fiber cotton for adsorbing lead ion colloidal-gold antibody. A linear invisible detection line T printed by a lead ion and carrier protein coupled solution and a linear contrast line C printed by a goat-anti-mouse IgG solution are successively arranged on the coating membrane, wherein the two lines are parallelly arranged. The colloidal gold chromatographic test strip for rapidly testing lead ion in the invention has strong specificity and high sensitivity, and can be used to more rapidly, sensitively and simply test lead ion residues. By the adoption of the test strip, the determination result is vivid, visual, accurate, simple and clear, and false positive and false negative human misjudgments are not easy to occur. The test strip provided by the invention can be used to save cost, has a wide application range, is convenient for popularization, and has an extensive market prospect as well as obvious economic and social benefits.

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to an enzyme-linked immunosorbent assay kit for detecting skeletal muscle troponin I of cattle or sheep, and a preparation method thereof and application thereof. The kit comprises an enzyme label plate coated with a capture antibody, a horseradish-peroxidase-labeled detection antibody, a skeletalmuscle troponin I standard solution of cattle or sheep, a substrate coloured solution, a stop solution, and a concentrated washing solution. The capture antibody is obtained by secretion of a hybridoma cell line 3A8 with the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell line 3D3 with the preservation number of CCTCC NO:C2018218. The kit has advantages of high sensitivity, high precision, high accuracy, and low cross-reaction rate and is suitable for large-scale sample detection.

The invention belongs to the technical field of animal virology and immunology, in particular to a double-antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting Japanese encephalitis virus antigens in swine, human and mosquitoes. Core reagents of the kit comprise a Japanese encephalitis virus E protein monoclonal antibody serving as a primary antibody and a rabbit polyclonal antibody serving as a secondary antibody. The invention discloses a preparation and purification method of the Japanese encephalitis virus E protein monoclonal antibody and the rabbit polyclonal antibody. The invention also discloses a detection method of the double-antibody sandwich ELISA. A hybridoma cell strain secreting the monoclonal antibody is preserved in China Center for Type Culture Collection with CCTCC NO. C2010114.

The invention discloses a tumor marker CD25 autoantibody and application of the tumor marker CD25 autoantibody, belonging to the technical field of immunology. The invention provides an amino acid sequence of antigen of immune response regulation gene CD25. The CD25 polypeptide antigen is used for detecting corresponding specific autoantibody in blood of lung cancer and esophageal cancer patients; and the autoantibody can be used as a tumor marker to evaluate risk level of occurrence of lung cancer and esophageal cancer. The antigen polypeptide and the antibody can be used for preparing early-stage tumor diagnostic reagent and developing target drugs for treating tumor.

A method for purifying abamectin medicines by the special immunoaffinity chromatographic column carrying immunoaffinity adsorbent is disclosed. Said adsorbent is composed of solid carrier and its coupled abamectin polyclonal or monoclonal antibody. It features that the chromatography is used to measure the contents of abamectin, having high correctness.

The invention relates to a malachite green collaurum detection card and production and application methods thereof, belonging to the technical field of immunology. The rapid diagnosis card comprises a case and a test strip; wherein the case is opened with a sample injecting hole and an observation window, the test strip is arranged in the case, the middle part of a PVC liner plate is superposed with a nitrocellulose membrane, the two ends are respectively superposed with an absorbent pad and a sample pad, the nitrocellulose membrane is overlapped and connected with the absorbent pad and the sample pad, a membrane containing malachite green monoclonal antibody immune collaurum compound is coated on a sample region, and a detection strip containing malachite green envelop antigen and a quality control strip containing goat anti mouse IgG are arranged on the nitrocellulose membrane. The invention is a rapid, accurate and sensitive diagnosis card, requires no special apparatus, has simple operation and can meet the detection requirements of food safety, fishery and detection mechanism.

The invention provides a nano antibody of clostridium difficile glutamate dehydrogenase, and an encoding sequence, a screening method and the application thereof and belongs to the technical field ofimmunology. The invention provides an amino sequence of the nano antibody of clostridium difficile glutamate dehydrogenase. By using a phage display technology, a sequence with a constant region specific gene is inserted into a vector of a phage encoding coat protein, after recombination expression, an exogenous gene expression product is fused with the phage encoding coat protein and demonstratedon the surface of phage to form a phage demonstration library, phage monocloning of the nano antibody is expressed through screening, and the nano antibody is prepared through sequencing. Phage-ELLSAidentification shows that the phage that the nano antibody is expressed on the surface can be specifically combined with clostridium difficile glutamate dehydrogenase antigen and has a good developing property, the result shows that the nano antibody has a good combination property with glutamate dehydrogenase, therefore, the nano antibody can be adopted to replace a common antibody applied to aclostridium difficile immunodetection kit.

The invention relates to a ractopamine immunogen, a ractopamine coating antigen and use thereof in colloid-gold test paper and belongs to the technical field of immunology. The immunogen and coating antigen are products of the coupling of the hapten of the connecting arm of a hydroxyl terminal far away from a nitrogen atom of the ractopamine with a carrier protein. The hapten is similar to a ractopamine counterpart in terms of molecular structure, stereochemistry and electronic distribution. The molecule of the hapten has an active group for coupling with a protein carrier, and the existence of the active group exerts on influences on the electronic distribution of the molecules of a material to be tested. When coupled with hemocyanin, the hapten enables an organism to produce a high-potency and high-specificity antibody against ractopamine and be absorbed onto a glass cellulose membrane; and the coating antigen constructed by coupling with human haemocyanin can be absorbed onto a nitrocellulose membrane for constructing the colloid-gold test paper.

The invention belongs to the field of immunological technique and discloses a T lymphocyte immunophenotyping method and a kit. The T lymphocyte immunophenotyping method provided by the invention comprises the following steps: taking different fluorescently-labeled antibodies and mixing the antibodies with a sample to be tested, carrying out incubation, carrying out flow cytometry detection to obtain test data, and analyzing the test data. The antibodies contain an anti-TCR alpha beta antibody, an anti-TCR gamma Delta antibody, an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD8 antibody, an anti-CD27 antibody and an anti-CD45RA antibody. By the above method, more comprehensive fine T lymphocyte immunophenotyping is realized. Few samples to be tested are required. The method is simple to operate. Required time is short. And the method has high accuracy and can widely be applied in lymphocyte subpopulation immunophenotyping.

The invention relates to the technical field of virus immunology, in particular to a method for preparing an anti-O-type foot-and-mouth disease virus monoclonal antibody and the application of the prepared antibody, and belongs to the field of foot-and-mouth disease prevention and control. The microorganism preservation number of the hybridoma cell line 6B8 capable of specifically secreting monoclonal antibody against type O foot-and-mouth disease virus of the present invention is: CCTCC No: C201049. The subtype of monoclonal antibody produced by the hybridoma cell line hybridoma cell line 6B8 is IgG1. The hybridoma cell line of the invention can be used in preparing reagents for diagnosing type O foot-and-mouth disease. The monoclonal antibody of the invention can be used in the preparation of reagents for diagnosing type O foot-and-mouth disease.

The invention relates to a reagent box to quantitatively detect multivesicular protein-1 content in human body fluid, using anti-human multivesicular protein-1 N-end monoclonal antibody and anti-human mulitvesicular protein-1 N-end polyclonal antibody, adopting immunologic technique to establish a double-antibody filled enzyme linked immunosorbent assay (ELISA) method of quantitatively detecting body fluid and multivesicular protein-1 content in tissue cracking liquid. Comparing the difference in multivesicular protein-1 content in body fluid, judge if the patient has autosomal dominant hereditary nephropathy and has an important reference value in clinic diagnosis of this disease and provides an effective route to prevent and cure this disease as soon as possible.

The invention discloses a multi-epitope peptide-loaded DC (dendritic cell) therapeutic vaccine for HCV (hepatitis C viruses), relating to the technical field of virology and immunology. The multi-epitope peptide-loaded DC (dendritic cell) therapeutic vaccine is characterized in that two CTL epitopes (namely, a NS4B (1793-1801) SMMAFSAAL and a P7 (774-782) AAWYIKGRL) are used for constructing recombinant adenoviruses, then the recombinant adenoviruses are used for infecting human dendritic cells so as to prepare a multi-epitope DC vaccine. Detection results indicate that the multi-epitope peptide-loaded DC (dendritic cell) therapeutic vaccine for HCV (hepatitis C viruses) disclosed by the invention has an immunogenicity.

The invention discloses an amino acid sequence for detecting a tumor marker P16 antigenic epitope and application of the amino acid sequence, and belongs to the technical field of immunology. The invention provides an antigenic amino acid sequence of a tumor anti-cancer gene P16. The P16 polypeptide antigen is used for detecting corresponding specific antigenic epitope in the blood of a patient with lung cancer and esophagus cancer; the antigenic epitope can be used as a tumor marker for estimating the degree of risk for occurring the lung cancer and the esophagus cancer. And the antigenic polypeptide and an antibody thereof can be used for preparing tumor early-diagnosis reagent and developing a targeted drug for treating the tumors.

An amino acid sequence for detecting autoantibody of tumor immune marker BIRC5 and an application belong to the technical field of immunology. The invention provides an antigen amino acid sequence of apotosis inhibition gene BIRC5. A BIRC5 polypeptide antigen is used for detecting corresponding specific autoantibody in blood of lung cancer patients, wherein the autoantibody can be used as a tumor marker for evaluating risk of generation of lung cancer. The polypeptide antigen and an antibody thereof can be used for preparing a tumor early-stage diagnosis reagent and developing a targeted drug which is used for treating tumors.

The invention discloses a rapid detection test strip for combined detection of cyromazine and melamine residues and a preparation method thereof. The nitrocellulose membrane (4) is coated with a rabbit anti-mouse IgG quality control line (5) and MA-BSA, CA - two detection lines for BSA (6); the anti-MA drug monoclonal antibody-colloidal gold marker and the anti-CA drug monoclonal antibody-colloidal gold marker coated on the binding pad (8). The present invention screens out anti-MA drug monoclonal antibodies and anti-CA drug monoclonal antibodies by using immunological technology, and through the preparation of colloidal gold and the exploration and research of colloidal gold labeling conditions, in the application of anti-MA drug monoclonal antibodies, anti-CA drug monoclonal antibodies Research on Cloning Antibody and Colloidal Gold Technology Establish colloidal gold test strips for dual detection of anti-MA and CA drugs, and measure their performance. The test strip has a wide range of detection samples, low false positives, sensitive, accurate and reliable detection, and is suitable for dual detection of CA and MA drug residues in animal edible tissues such as muscle, liver and kidney and milk.

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to a colloidal gold chromatography test strip for detecting skeletal muscle troponin I of cattle orsheep, and a preparation method and application thereof. The test strip comprises a bottom plate, a sample pad, a colloidal gold pad, a coating film and an absorbent pad. Protective films are arranged at two ends of the test strip. The sample pad, the colloidal gold pad, the coating film and the absorption pad are pasted on the bottom plate successively. The colloidal gold pad is coated with a colloidal-gold-labeled capture antibody. The coating film is provided with an invisible detection line printed by a detecton antibody solution and an invisible quality control line printed by a goat anti-mouse IgG solution. The capture antibody is obtained by secretion of a hybridoma cell strain 3A8 having the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell strain with the preservation number of CCTCC NO:C2018218. The colloidal gold chromatography test strip having characteristics of great convenience, rapidity, visibility, strongtimeliness, wide application range, and low cost is convenient to promote and apply.

The invention relates to clenbuterol immunogen, coatingen and application thereof in colloidal gold test paper, and belongs to the technical field of immunology. The immunogen and the coatingen are products of hapten coupling carrier proteins obtained by reaction of clenbuterol and 4-bromobutyric acid. The hapten is similar to the clenbuterol corresponding body on molecular structure, stereo chemistry and electronic distribution. The hapten molecules have active groups convenient for coupling with protein carriers, and the presence of the active groups has no influence on the electronic distribution of molecules of an object to be detected. After the hapten is coupled with hemocyanin, the body can produce clenbuterol-orientated antigens with high titer and good specificity, wherein the antigens are adsorbed on a glass cellulose membrane; and the coatingen constructed after coupling the human albumin can be adsorbed on a nitrocellulose membrane to construct the clenbuterol colloidal gold test paper.

The invention relates to a veterinarian virus molecular biology technology and an immunology technology and discloses a thermal-stability Newcastle disease virus novel (gene VIII type) attenuated vaccine candidate strain NDV / rAHR09 and a construction method thereof. The preservation number of the thermal-stability Newcastle disease virus attenuated vaccine candidate strain is CCTCC (China Center for Type Culture Collection) NO: V201739. A research shows that the NDV / rAHR09 strain has good thermal stability and still keeps blood coagulation activity and infection capability at 56 DEG C after 60min; an immunity test shows that the thermal-stability Newcastle disease virus attenuated vaccine candidate strain has a good immune protection effect on a Newcastle disease virulent strain; the strain, a common vaccine strain LaSota and a heat-resisting V4 strain are inoculated into 1-day-age SPF chickens respectively and the antibody valence of the chickens is detected every week; a test resultproves that the antibody valence of the chickens immunized by the virulent strain is remarkably higher than that of the LaSota strain and the V4 strain and the toxin-invading protection rate on prevalent virulent strains is 100 percent.

The invention belongs to the biological engineering and immunology technology fields and specifically relates to a linear epitope minimum motif peptide of human zona pellucida (huZP) protein huZP1 and huZP3, and ZP chimeric peptides. In particular, the invention provides the minimum epitope amino acid sequence on the huZP1 and the huZP3 proteins for detecting ZP antibodies (Leading to the sterility or premature ovarian failure of women) and the 8-peptide sequence containing the epitope minimum motif and of truncated GST188 carrier protein fusion expression. The epitope minimum motif peptide of the huZP1 with immunogenicity can be identified by a rabbit anti-pig ZP antiserum and the 5-epitope minimum peptide motif of huZP3 can be identified by a rabbit antibody human ZP3 antiserum, to prove the value of the epitope minimum motif peptide when used as a single-epitope or multi-epitope peptide contraception antigen or when used as a single-epitope or multi-epitope fusion expression antigen for detecting the ZP antibodies of humans.

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

The invention mainly belongs to the technical field of tumor immunology, and particularly relates to a human urinary bladder carcinoma marker AG-CD71 and a monoclonal antibody ABC71 for preventing AG-CD71. The human urinary bladder carcinoma marker AG-CD71 provided by the invention is an abnormal glycosylated transferrin receptor TFRC; the abnormal glycosylated transferrin receptor TFRC refers tothat the TFRC carriers a saccharide structure Fucal-4(GlcNAcb1-3) as an epitope; the invention provides an antibody for the human urinary bladder carcinoma marker AG-CD71; the antibody is a specific monoclonal antibody ABC71 for preventing the human urinary bladder carcinoma marker AG-CD71; the monoclonal antibody is secreted from a hybridoma cell strain of which the preservation number is CGMCC No.14312. Cell and histology levels show that the ABC71 is capable of specifically recognizing human urinary bladder carcinoma cells and human urinary bladder carcinoma tissue. The monoclonal antibodyABC71 for preventing the human urinary bladder carcinoma has an intense positive reaction with human urinary bladder carcinoma tissue and has a negative reaction with normal human urinary bladder tissue.

The invention discloses a synthesis method of a half-antigen and a complete antigen of tulathromycin and belongs to the technical field of immunology. The method synthesizes the half-antigen of tulathromycin by carrying out hydroxyl on tulathromycin by a succinic anhydride method and introducing free carboxyl. Derivated free carboxyl reacts with primaquine of bovine serum albumin to form an amido bond for coupling by an active ester method to form the complete antigen of tulathromycin. After the antigens are used for immunizing a Balb / c mouse for three times, the valence can reach 1:8000. The antigens have wide practical application prospects.

A method for purifying salbutamol and / or clenbuterol by the special immunoaffinity chromatographic column carrying immunoaffinity adsorbent is disclosed. Said adsorbent is composed of solid carrier and its coupled salbutamol polyclonal or monoclonal antibody. It features that the chromatography is used to measure the contents of salbutamol and clenbuterol, having high correctness.

The invention relates to a colloidal gold sensitization chromatography test strip for quickly detecting copper ions, which belongs to the technical field of immunology, and comprises a lining board, a sample cushion, a gold-marking combined cushion, a sensitization cushion, a coating film and a water absorption cushion, wherein the sample cushion, the gold-marking combined cushion, the sensitization cushion, the coating film and the water absorption cushion are sequentially jointed on the lining board; the gold-marking combined cushion is made of glass fiber cotton and used for absorbing small gold mark copper ion antibodies; the sensitization cushion is made of glass fiber cotton and used for absorbing large gold mark goat anti-mouse IgG (immunoglobulin G); and the coating film is provided with a linear invisible detection line T and a linear invisible control line C which are arrayed in parallel, wherein the linear invisible detection line T is printed by the solution formed by the coupling of the copper ions and carrier protein, and the linear invisible control line C is printed by the goat anti-mouse IgG solution. The colloidal gold sensitization chromatography test strip for quickly detecting the copper ions, provided by the invention, has the advantages of strong specificity and high sensitivity, can detect the residue of the copper ions more quickly, sensitively, simply and conveniently, is lifelike, intuitive, accurate, simple and clear in result determination, is not easy to appear human misjudgment such as false positive, false negative and the like, saves the expense, has wide application range, facilitates the popularization, and has wide market prospect and remarkable economic and social benefits.

The invention relates to the technical fields of veterinarian virus molecule biology and immunity, and discloses a recombinant gene VIII-type Newcastle disease virus attenuated strain NDV-rHR09-LaSota-HN. A preservation number of the attenuated strain NDV-rHR09-LaSota-HN is CCTCC (China Center For Type Culture Collection) NO: V201830. The research shows that the NDV-rHR09-LaSota-HN strain is weakin toxicity, the MDT is greater than 120h, the ICPI value is 0.026, an immunity test proves that the Newcastle disease virus attenuated strain has a good immunity protection effect on Newcastle disease virulent strain; and compared with the conventional vaccine strain LaSota, the NDV-rHR09-LaSota-HN strain is inoculated to one-day SPF chicken, and the antibody level and the protection effect on the prevalent virulent strain counteracting toxic substances of the chicken after three weeks are better or not poorer than a reference group.

The invention relates to immunogen and coatinggen of chloramphenicol and application thereof in collaurum test paper, and belongs to the technical field of immunology. The immunogen and the coatinggen are a product of hapten coupling carrier protein obtained by oxidizing the chloramphenicol through potassium permanganate. The hapten is similar to a chloramphenicol counterpart in the aspects of molecular structures, stereochemistry and electron distribution; and hapten molecules have active groups which are convenient to couple with a protein carrier, and the active groups do not influence the electron distribution of analyte molecules. After hemocyanin is coupled by the hapten, organisms can generate antibodies with high valence and good specificity aiming at the chloramphenicol, and the antibodies are adsorbed to glass cellulose membranes; and the coatinggen constructed by coupling human serum protein can be adsorbed to nitrocellulose membranes and is used for constructing the chloramphenicol collaurum test paper.

The invention belongs to the technical field of clinical immunology, and discloses a monoclonal antibody prepared from African swine fever virus truncated protein p54 and application of the monoclonalantibody. The monoclonal antibody clone antibody 4-4C is finally obtained by immunizing the effectively truncated African swine fever virus protein p54 and systematically screening hybridoma. The obtained monoclonal antibody can react with the African swine fever totivirus to meet the basic requirements of the monoclonal antibody; in addition, the monoclonal antibody is good in specificity and has no cross reaction with other proteins of the African swine fever virus; in addition, after the monoclonal antibody is detected by Elisa, the titer of the monoclonal antibody reaches up to 1600000; finally, the monoclonal antibody has a blocking effect, the established blocking Elisa detection method has a detection effect equivalent to that of a Spanish detection kit recommended by OIE, the detection time is shortened, and the monoclonal antibody has the capacity of detecting the antibody level of African swine fever for pig farms.