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Test strip for rapidly detecting trace erythrosine and preparation method

A technology of trace erythritis and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of being unable to detect on-site, difficult to promote, cumbersome and time-consuming, etc., to protect the health of consumers and save the cost of expensive instruments and equipment , low-cost effect

Active Publication Date: 2013-12-18
河南百奥生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for the determination of erythrosine residues in food at home and abroad, mainly using physical and chemical analysis methods, including high performance liquid chromatography (HPLC), liquid chromatography / mass spectrometry (LC / MS), gas chromatography Chromatography / mass spectrometry (GC / MS), etc. These methods have high sensitivity and specificity for the detection of erythrosine, but the samples need to undergo a series of complicated processing and purification, which is tedious and time-consuming, from sample pretreatment to detection The results will take at least 2 days; at the same time, these detection methods require large-scale specialized equipment and professional technicians to operate, and cannot be tested on-site. The timeliness is poor and it is difficult to promote

Method used

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  • Test strip for rapidly detecting trace erythrosine and preparation method
  • Test strip for rapidly detecting trace erythrosine and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: test strip and preparation method for rapid detection of trace amounts of erythrosine, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic sheet strips, the adsorption fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-erythrosine monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose Membrane, water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the junctions cross and penetrate each other. On the cellulose membrane layer 4, there are detection blots 6 and control blots 7, the detection blots are printed with erythrosin-coupled bovine serum albumin (BSA) solution, and the control blots are printed with goat anti-antibody solution. The blots are arranged in parallel to form blotted bands" ︱︱ ...

Embodiment 2

[0049] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the polyclonal antibody against erythrosin is adsorbed on the gold-labeled antibody fiber layer, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane layer is made of Pure cellulose membrane, both the test blot and the control blot are "ten", and the protective film covering the handle end above the water-absorbing material layer is blue.

[0050] For the detection of solid soups, take 2g of a homogeneous sample, put it in a 50mL centrifuge tube, add 20mL of sample diluent, mix well, add 10mL of n-hexane, shake for 10min, centrifuge at 5000r / min for 10min, and take the supernatant for detection.

[0051] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane layer " ten ", indicating that the test result is positive, indicating that the sample to be tested contains erythrosine; (b) negative if ther...

Embodiment 3

[0053] Embodiment three: test strip structure is basically the same as embodiment one, and the difference is that the adsorption fiber layer is made of PVDF membrane, and the carrier protein solution coupled with erythrosine is chicken ovalbumin (OVA), and the control blot is used The rabbit anti-mouse IgG antibody solution is blotted on a cellulose membrane. The cellulose membrane layer is made of carboxylated cellulose membrane. The protective film at the handle end covering the water-absorbing material layer is green. Both the detection blot and the control blot are "┬ ". For the detection of jam, take 2g of a homogeneous sample, put it in a 50mL centrifuge tube, add 20mL of sample diluent, mix and shake for 10min, centrifuge at 5000r / min for 10min, and take the supernatant for detection. Result determination, operation and preparation method are all the same as in Example 1 and will not be repeated.

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Abstract

The invention relates to a test strip for rapidly detecting trace erythrosine and a preparation method. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer and a protection layer is fixed on the adsorption layer, wherein the adsorption layer is composed of an adsorption fiber layer, a gold-labeled antibody layer, a cellulose membrane layer and a water absorption material layer in sequence from a testing end; the cellulose membrane layer is provided with a detection print printed by an erythrosine coupled carrier protein solution and a contrast print printed by a goat or rabbit anti-mouse IgG (Immunoglobulin G) solution or a goat anti-rabbit IgG solution; an erythrosine gold-labeled antibody is a colloidal gold labeled erythrosine monoclonal antibody or polyclonal antibody; the erythrosine coupled carrier protein solution is a coupled compound solution of erythrosine and a carrier protein. The test strip provided by the invention is strong in specificity and high in sensitivity and is simple, visualized and accurate; an applicable range is wide and the cost is low; therefore, the test strip is easy to popularize and apply.

Description

technical field [0001] The invention relates to a technique for detecting trace pigments, in particular to a test strip and a preparation method for rapidly detecting trace erythrosine. technical background [0002] Erythrosin, also known as cherry red, tetraiodofluorescein, food coloring No. 3, molecular formula is C 20 h 6 I 4 Na 2 o 5 ·H 2 O, common red or reddish-brown granules or powder, odorless, soluble in water, soluble in ethanol, propylene glycol and glycerin, insoluble in oil, good heat resistance, alkali resistance, redox resistance, bacteria resistance and light resistance Poor resistance, precipitation when encountering acid, poor hygroscopicity. Erythrosine has good dyeing ability, especially for protein dyeing. According to its properties, the coloring power is stronger than other synthetic pigments in baked foods requiring high temperature and alkaline and neutral foods. The Food and Agriculture Organization of the United Nations and the World Health...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/544G01N33/531
Inventor 张改平邢广旭刘珍王栋胡骁飞王方雨赵东
Owner 河南百奥生物工程有限公司
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