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Colloidal gold chromatographic test strip for rapidly testing lead ion

A technology for detecting test strips and lead ions, which is applied in the field of immunology, and achieves the effect of being easy to popularize and apply, easy to popularize, and has a wide range of applications

Inactive Publication Date: 2012-04-25
王利兵 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of immunological detection methods to detect heavy metal ions in the environment is still in the experimental stage of the laboratory

Method used

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  • Colloidal gold chromatographic test strip for rapidly testing lead ion
  • Colloidal gold chromatographic test strip for rapidly testing lead ion
  • Colloidal gold chromatographic test strip for rapidly testing lead ion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, preparation of lead ion colloidal gold chromatography detection test strip

[0017] To make lead ion detection test strips, it is first necessary to prepare a carrier protein coupled to lead ions for the preparation of the corresponding detection line (T line) and antibody; and it is necessary to prepare lead gold-labeled antibody for the preparation of the corresponding gold standard Antibody fiber cotton; in addition, it is necessary to prepare goat anti-mouse IgG antibody for the preparation of the control line (C line).

[0018] 1. Coupling of lead to carrier protein

[0019] Preparation of immunoantigen lead-p-thiocyanbenzyl-ethylenediaminetetraacetic acid-keyhole limpet hemocyanin (Pb-ITCBE-KLH).

[0020] First react 2mg of ITCBE and 4.5mg of KLH protein in 3mL, pH9.4 HEPES (hydroxyethylpiperazine ethylsulfuric acid) buffer solution for 24h, then ultrafilter for 10min at 4°C and 6000r / min Remove uncoupled ITCBE, wash the retentate with HEPES buffer...

Embodiment 2

[0034] Embodiment 2, detection sample pretreatment and sample detection

[0035] Spray the selected concentration of coating antigen and goat anti-mouse IgG on the coating film as the test line (T) and control line (C) respectively, and dry in an oven at 37°C for 10 min. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a backing plate, on which a sample pad, a gold standard binding pad, a coating film and an absorbent pad are glued in sequence. Cut the affixed board into 3mm wide strips, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.

[0036] Pre-treatment of test samples into digestive juice: take 1.0 g of homogeneous pork sample, add 2 mL of nitric acid into a polytetrafluoroethylene crucible, and place at room temperature for 4 hours. Transfer the sample to the digestion tube, rinse the crucible with 2 mL of nitric acid several times...

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Abstract

A colloidal gold chromatographic test strip for rapidly testing lead ion belongs to the field of immunological technique. The test strip provided by the invention is composed of a liner plate and a sample pad, a colloidal-gold combination pad, a coating membrane and a water absorption pad which are successively connected on the liner plate. The colloidal-gold combination pad is glass fiber cotton for adsorbing lead ion colloidal-gold antibody. A linear invisible detection line T printed by a lead ion and carrier protein coupled solution and a linear contrast line C printed by a goat-anti-mouse IgG solution are successively arranged on the coating membrane, wherein the two lines are parallelly arranged. The colloidal gold chromatographic test strip for rapidly testing lead ion in the invention has strong specificity and high sensitivity, and can be used to more rapidly, sensitively and simply test lead ion residues. By the adoption of the test strip, the determination result is vivid, visual, accurate, simple and clear, and false positive and false negative human misjudgments are not easy to occur. The test strip provided by the invention can be used to save cost, has a wide application range, is convenient for popularization, and has an extensive market prospect as well as obvious economic and social benefits.

Description

technical field [0001] The invention relates to a colloidal gold chromatography test strip for rapidly detecting lead ions, a preparation method and application thereof, and belongs to the technical field of immunology. Background technique [0002] Lead is a major raw material in current industrial production, and it is very harmful to the human body. Lead poisoning can cause neurasthenia and indigestion, and lead encephalopathy can occur in severe cases, resulting in epilepsy, dementia or mental irritability, vision loss or even blindness. In 1923, lead was added to gasoline as an antiknock agent, which accelerated global lead pollution. Lead pollution has spread across the globe, and the amount of lead in the ice of Greenland and Antarctica has increased exponentially. Therefore, it can be said that it is difficult to find a piece of "pure land" in the world today where the soil lead content is not affected by human activities. [0003] my country's groundwater quality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 王利兵胥传来邢常瑞
Owner 王利兵
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