Test strip for rapidly detecting trace sulfachlorpyridazine and preparation method thereof

Abstract

The invention relates to a test strip for rapidly detecting trace sulfachlorpyridazine and a preparation method thereof. According to the test strip, the bottom layer is a support layer, the middle layer is an adsorption layer, and a protection layer is fixed on the adsorption layer; the adsorption layer sequentially comprises an adsorption fibre layer, a gold-labelled antibody fibre layer, a cellulose membrane layer and a water-absorbing material layer from a test end; a detection imprint printed by carrier protein solution coupled with sulfachlorpyridazine, and a control imprint printed by goat anti-mouse IgG antibody solution or rabbit anti-mouse IgG antibody solution or goat anti-rabbit IgG antibody solution are formed on the cellulose membrane layer; a gold-labelled antibody is a colloidal gold-labelled sulfachlorpyridazine antibody. The test strip disclosed by the invention is strong in specificity, high in sensitivity, simple and convenient, intuitive, accurate, wide in application range, low in cost, simple and convenient to operate, capable of meeting the needs of personnel in different levels, wide in market prospect, and easy to popularize and apply.

Description

technical field

[0001] The invention belongs to the field of immunochemical detection of veterinary drug residues, in particular to an immunocolloidal gold test strip for rapidly detecting trace amounts of sulfachloropyridazine and a preparation method thereof. technical background

[0002] Sulfachloropyridazine (sulfachloropyridazine, SPDZ) molecular formula C 10 h 9 ClN 4 o 2 S, with a molecular weight of 284.72, is a kind of synthetic antibacterial drug that inhibits nucleic acid synthesis. It belongs to a broad-spectrum antibacterial drug. It has the characteristics of low toxicity and easy absorption after oral administration. Antibacterial effect. Due to the low price and effective antibacterial effect of sulfa drugs, they are widely used in animal husbandry as preventive and therapeutic drugs. However, the unreasonable use or even abuse of sulfachloropyridazine will cause the emergence of drug-resistant strains on the one hand, leading to the pollution of animal ...

Examples

Embodiment 1

[0035] Embodiment one: the test strip of rapid detection trace sulfachloropyridazine, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic thin strips, the adsorption fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-sulfachloropyridazine monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose The plain film, the water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the intersections cross and penetrate each other. A detection blot 6 and a control blot 7 are arranged on the cellulose membrane layer 4, the detection blot is printed with bovine serum albumin (BSA) solution coupled with sulfachloropyridazine, and the control blot is printed with goat anti-antibody solution, Two blots aligned in parallel to form blotte...

Embodiment 2

[0054] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the polyclonal antibody against sulfachloropyridazine is adsorbed on the gold-labeled antibody fiber layer, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane The layer is made of pure cellulose film, the test blot and the control blot are both "ten", and the protective film at the handle end covering the water-absorbing material layer is blue.

[0055]For the detection of milk samples, the milk samples can be directly diluted with physiological saline to make a 1:2~5 suspension of the sample to be tested;

[0056] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there are two brown-red blots on the cellulose membrane" ten ", expression test ...

Embodiment 3

[0057] Embodiment three: the sulfachloropyridazine test paper strip is basically the same as embodiment one, the difference is:

[0058] The absorbent fiber layer is made of PVDF membrane, the carrier protein solution coupled with sulfachloropyridazine is chicken ovalbumin (OVA), and the control blot is printed on the cellulose membrane with rabbit anti-mouse IgG antibody solution, and the cellulose membrane The layer is made of carboxylated cellulose film, the protective film on the handle end covering the water-absorbing material layer is green, and both the detection blot and the control blot are "┬".

[0059] Detection of blood samples: Extract serum and dilute it with normal saline to make a 1:2-10 sample to be tested.

[0060] Result judgment: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there is a blot on t...

technical field

[0001] The invention belongs to the field of immunochemical detection of veterinary drug residues, in particular to an immunocolloidal gold test strip for rapidly detecting trace amounts of sulfachloropyridazine and a preparation method thereof. technical background

[0002] Sulfachloropyridazine (sulfachloropyridazine, SPDZ) molecular formula C 10 h 9 ClN 4 o 2 S, with a molecular weight of 284.72, is a kind of synthetic antibacterial drug that inhibits nucleic acid synthesis. It belongs to a broad-spectrum antibacterial drug. It has the characteristics of low toxicity and easy absorption after oral administration. Antibacterial effect. Due to the low price and effective antibacterial effect of sulfa drugs, they are widely used in animal husbandry as preventive and therapeutic drugs. However, the unreasonable use or even abuse of sulfachloropyridazine will cause the emergence of drug-resistant strains on the one hand, leading to the pollution of animal ...

Examples

Embodiment 1

[0035] Embodiment one: the test strip of rapid detection trace sulfachloropyridazine, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic thin strips, the adsorption fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-sulfachloropyridazine monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose The plain film, the water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the intersections cross and penetrate each other. A detection blot 6 and a control blot 7 are arranged on the cellulose membrane layer 4, the detection blot is printed with bovine serum albumin (BSA) solution coupled with sulfachloropyridazine, and the control blot is printed with goat anti-antibody solution, Two blots aligned in parallel to form blotte...

Embodiment 2

[0054] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the polyclonal antibody against sulfachloropyridazine is adsorbed on the gold-labeled antibody fiber layer, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane The layer is made of pure cellulose film, the test blot and the control blot are both "ten", and the protective film at the handle end covering the water-absorbing material layer is blue.

[0055]For the detection of milk samples, the milk samples can be directly diluted with physiological saline to make a 1:2~5 suspension of the sample to be tested;

[0056] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there are two brown-red blots on the cellulose membrane" ten ", expression test ...

Embodiment 3

[0057] Embodiment three: the sulfachloropyridazine test paper strip is basically the same as embodiment one, the difference is:

[0058] The absorbent fiber layer is made of PVDF membrane, the carrier protein solution coupled with sulfachloropyridazine is chicken ovalbumin (OVA), and the control blot is printed on the cellulose membrane with rabbit anti-mouse IgG antibody solution, and the cellulose membrane The layer is made of carboxylated cellulose film, the protective film on the handle end covering the water-absorbing material layer is green, and both the detection blot and the control blot are "┬".

[0059] Detection of blood samples: Extract serum and dilute it with normal saline to make a 1:2-10 sample to be tested.

[0060] Result judgment: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there is a blot on t...

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