Olaquindox metabolite residue rapid detection test strip
A technology for detecting test strips and metabolites, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable sample screening, low recovery rate, cumbersome operation, etc., so as to prevent false negatives and false positives , low cost and wide application range
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[0016] To prepare rapid test strips for the detection of olaquindox metabolite residues, it is first necessary to prepare carrier protein and gold-labeled antibody coupled to olaquindox metabolites, so as to prepare detection blots and gold-labeled antibody fibers; secondly, it is necessary to prepare goat anti or rabbit anti-mouse IgG Antibodies, used to prepare control blots.
[0017] 1. Olaquindox metabolite 3-methyl-quinoline-2-carboxylic acid (MQCA) synthesis
[0018] (1) Production of silica gel thin layer board
[0019] Take 12 g of silica gel, dissolve it in 40 mL of 0.5% mass concentration of hydroxymethylcellulose sodium solution, fully stir it into a paste with a glass rod, oscillate ultrasonically for 1 minute, and spread it evenly on multiple pieces of 0.3×8 cm glass After placing it in a cool place to dry naturally, place it in an oven at 105°C for 1 hour to activate, and put the obtained silica gel thin-layer board in a drying oven for later use;
[0020] (2) ...
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