Olaquindox metabolite residue rapid detection test strip

A technology for detecting test strips and metabolites, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable sample screening, low recovery rate, cumbersome operation, etc., so as to prevent false negatives and false positives , low cost and wide application range

Inactive Publication Date: 2014-05-14
JIANGSU WISE SCI & TECH DEV
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Problems solved by technology

[0003] At present, the MQCA residue analysis reported in the literature can be analyzed and determined by HPLC and HPLC-MS / MS. These methods are generally hydrolyzed first, then purified by solid-phase extraction column and then tested on the machine. High detection limit and low recovery rate are common. The shortcomings of being low, cumbersome and time-consuming, are not suitable for the screening of a large number of samples on site

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  • Olaquindox metabolite residue rapid detection test strip
  • Olaquindox metabolite residue rapid detection test strip
  • Olaquindox metabolite residue rapid detection test strip

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Embodiment Construction

[0016] To prepare rapid test strips for the detection of olaquindox metabolite residues, it is first necessary to prepare carrier protein and gold-labeled antibody coupled to olaquindox metabolites, so as to prepare detection blots and gold-labeled antibody fibers; secondly, it is necessary to prepare goat anti or rabbit anti-mouse IgG Antibodies, used to prepare control blots.

[0017] 1. Olaquindox metabolite 3-methyl-quinoline-2-carboxylic acid (MQCA) synthesis

[0018] (1) Production of silica gel thin layer board

[0019] Take 12 g of silica gel, dissolve it in 40 mL of 0.5% mass concentration of hydroxymethylcellulose sodium solution, fully stir it into a paste with a glass rod, oscillate ultrasonically for 1 minute, and spread it evenly on multiple pieces of 0.3×8 cm glass After placing it in a cool place to dry naturally, place it in an oven at 105°C for 1 hour to activate, and put the obtained silica gel thin-layer board in a drying oven for later use;

[0020] (2) ...

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Abstract

Belonging to the field of food safety rapid detection, the invention relates to an olaquindox metabolite residue rapid detection test strip. The test strip comprises a bottom plate, and a sample pad, a conjugate release pad, a reaction film and a water absorption pad that are attached to the bottom plate and are closely connected. Part of the conjugate release pad is covered by the sample absorption pad. The reaction film is coated with a detection line composed of an olaquindox metabolite-carrier protein conjugate and is coated with a quality control line composed of goat-anti-mouse IgG. The conjugate release pad is coated with a colloidal gold marker of the olaquindox metabolite. The test strip can extend the detection result observation time, the sample absorption pad can fully absorb a detection liquid and fully contact a gold-labelled antibody so as to react completely, and then chromatography to the reaction film is carried out to undergo reaction, thus effectively reducing error. The test strip also can prevent some interference components in the detected sample from inactivating the protein in the gold-labelled antibody to affect combination of the gold-labelled antibody and a coating antigen.

Description

technical field [0001] The invention relates to a test strip for rapid detection of residual metabolites of olaquindox, belonging to the field of rapid detection of food safety. technical background [0002] Olaquindox is a pharmaceutical additive with growth-promoting effect, which has antibacterial and protein assimilation effects, and also has growth-promoting effect. It has a strong effect on Gram-negative bacteria such as Pasteurella, Salmonella pullorum, Escherichia coli and Proteus, and it also has inhibitory effect on Gram-negative bacteria such as Staphylococcus aureus, Streptococcus and Treponema. It can promote protein assimilation, increase lean meat rate, promote the growth of livestock and poultry, and improve feed conversion rate. It is widely used in the growth promotion of pigs, cattle, chickens, and sheep, antibacterial treatment and prevention and treatment of aquatic animal diseases. Used in feed, it can improve the utilization rate of feed through the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/54386G01N33/94
Inventor 杜道林邢海龙
Owner JIANGSU WISE SCI & TECH DEV
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