Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip

A technique for detecting dichlorophenoxyacetic acid and test paper, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of missed detection and false detection, and achieve the effect of low cost, low investment and high marking rate

Inactive Publication Date: 2014-10-15
HENAN UNIVERSITY OF TECHNOLOGY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since ELISA is a highly sensitive detection method, different operators often have different results, so this method often causes artificial false detection and missed detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip
  • 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025]4. Preparation of goat or rabbit anti-mouse IgG

[0026] Extract mouse serum IgG with saturated ammonium sulfate, take 1 part of mouse serum and 2 parts of PBS (pH 7.2) and mix well, add an equal volume of saturated ammonium sulfate and mix well, put in 4°C refrigerator for 2 h, 4°C, 1200 r / min Centrifuge for 15 min, discard the supernatant; dissolve the precipitate with an appropriate amount of PBS (pH7.2), add saturated ammonium sulfate to a final concentration of 33%, place in a refrigerator at 4°C and dialyze with PBS (pH7.2) overnight, and change the medium 2 to 3 times , centrifuge at 12,000 r / min at 4°C for 15 min, collect the supernatant, measure its protein concentration with a UV spectrophotometer, and immunize healthy sheep or 3 to 4 times in rabbits, 10 days after the last immunization, blood was collected from the vein, and the serum antibody titer was determined to be above 1:2000 by ELISA, blood was collected from the heart or carotid artery, and the seru...

Embodiment 1

[0032] see figure 1 and figure 2 . In the figure 1 is the support layer, made of plastic thin strips, 2 is the fiber layer at the test sample end, made of glass wool, 3 is the fiber layer of gold-labeled antibody (W1) adsorbed with 2,4-dichlorophenoxyacetic acid , according to the preparation method described in the above specific embodiment 3, prepare the gold-labeled antibody (W1) glass wool with 2,4-dichlorophenoxyacetic acid monoclonal antibody adsorbed, 4 is the cellulose film layer, this embodiment adopts Nitrocellulose membrane, 5 is a water-absorbing material layer, made of water-absorbing filter paper, each layer of numbering 2, 3, 4, and 5 is pasted on the plastic sheet strip 1 from left to right, and the fibers at the junction are interpenetrated with each other. On the nitrocellulose membrane layer 4, 6 is the detection blot "|" printed with the carrier protein BSA solution coupled with 2,4-dichlorophenoxyacetic acid, and 7 is printed with the goat anti-mouse Ig...

Embodiment 2

[0034] 2,4-Dichlorophenoxyacetic acid residual rapid detection test strip structure is basically the same as that of Example 1, the difference is that the support layer 1 is made of non-absorbent hard paper strips, and the test end fiber layer 2 is made of nylon film. 2,4-Dichlorophenoxyacetic acid gold-labeled antibody fiber layer 3 is adsorbed with colloidal gold-labeled 2,4-dichlorophenoxyacetic acid polyclonal antibody, cellulose membrane layer 4 is made of pure cellulose membrane, invisible detection blot 6 The carrier protein solution coupled with 2,4-dichlorophenoxyacetic acid was ferritin, and the invisible control blot 7 was prepared on a cellulose membrane with a rabbit anti-mouse IgG solution. Others including test sample preparation, operation method and result judgment are the same as the operation method in Embodiment 6 and will not be repeated.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a herbicide detection device, and especially relates to a 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip. The 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip comprises a bottom supporting layer, a middle adsorption layer fixed on the supporting layer and an outer protective layer fixed on the adsorption layer, the adsorption layer successively comprises, from a test sample end, a fiber layer, a fiber layer adsorbing gold immnnochromatography carrier protein Xi coupled with 2,4-dichlorphenoxyacetic acid, a cellulose film layer and a water absorption material layer at the handle end, the cellulose film layer is provided with a straight line type detection imprinting printed by a 2, 4-dichlorphenoxyacetic acid carrier protein solution and a straight line type comparison imprinting printed by a sheep or rabbit anti-mouse IgG solution, and the detection imprinting and the comparison imprinting are in parallel and combined arrangement of "I I". The 2, 4-dichlorphenoxyacetic acid residue rapid detection test paper strip has the advantages of strong specificity, high sensitivity, simple and fast operation, vivid, intuitive and accurate detection result display, low cost, less investment, no need of special equipment and professional staff, and easy popularization and implementation.

Description

technical field [0001] The invention relates to a device for detecting herbicides, in particular to a test strip for rapid detection of 2,4-dichlorophenoxy (abbreviated as 2,4-D) residues. Background technique [0002] As the saying goes: "Food is the most important thing for the people", food safety is a major issue related to the vital interests of millions of people and the national economy and people's livelihood. However, with the modernization and high technology of animal husbandry, the problem of food safety is becoming more and more serious, which is frightening. In order to eliminate weeds and pests, some people use a large amount of toxic or even highly toxic pesticides, causing pesticide residues in vegetables and fruits to exceed the standard, posing a serious threat to human health and life. For example, 2,4-dichlorophenoxyacetic acid is a widely used herbicide in the world and is also a synthetic plant hormone with similar physiological effects to auxin. It ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/531G01N33/74
Inventor 席俊
Owner HENAN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com