Test strip for quickly detecting oxolinic acid and preparation method thereof

The technology of oxaquinic acid and test strips is applied in the field of immunological detection of veterinary drug residues, which can solve the problems of inability to conduct on-site detection in large-scale batch detection, affecting the accuracy of analysis results, and high requirements for instrument maintenance, achieving obvious economic benefits and Social benefits, saving expensive instruments and equipment costs, and low storage temperature requirements

Active Publication Date: 2014-01-22
河南百奥生物工程有限公司 +1
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting oxolinic acid residues at home and abroad mainly use physical and chemical analysis methods, including high performance liquid chromatography (HPLC), liquid chromatography / mass spectrometry (LC / MS), gas chromatography / mass spectrometry ( GC / MS), etc., these methods have certain defects: (1) The pretreatment process of the sample to be tested is complicated, tedious and time-consuming; (2) Expensive instruments and equipment are required, and the promotion and use are limited; (3) The professionalism is limited. The quality requirements are high, and certain relevant experience is required to obtain reliable results; (4) The maintenance requirements of the instrument are high, and the quality of maintenance directly affects the accuracy of the analysis results; (5) The testing cost is high; (6) On-site testing and large-scale Large-scale batch detection, and poor timeliness

Method used

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  • Test strip for quickly detecting oxolinic acid and preparation method thereof
  • Test strip for quickly detecting oxolinic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Test strips and preparation methods for rapid detection of trace amounts of oxolinic acid, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic thin strips, the absorbent fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-oxquinic acid monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose The water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the junctions cross and penetrate each other. On the cellulose membrane layer 4, there are detection blots 6 and control blots 7, the detection blots are printed with bovine serum albumin (BSA) solution coupled with oxolinic acid, and the control blots are blotted with rabbit anti-mouse IgG antibody As a result, two imprints are arranged in parallel to for...

Embodiment 2

[0050] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the gold-labeled antibody fiber layer is adsorbed with polyclonal antibodies against oxquinic acid, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane layer is made of The pure cellulose membrane, the detection imprinted band and the invisible control imprinted band are all "ten", and the protective film at the handle end covering the water-absorbing material layer is blue.

[0051] The sample to be tested is chicken liver, the sample is cut into pieces, smashed, and then centrifuged at 4000r / min for 5min, and the upper layer juice is taken as the sample to be tested;

[0052] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that oxolinic acid is contained in the sample to be tested; (b) negative if there are two brown-red blo...

Embodiment 3

[0054] Embodiment 3: The structure of the test strip is basically the same as that of Embodiment 1, except that the absorbent fiber layer is made of polyvinylidene fluoride membrane (PVDF), and the carrier protein solution coupled with oxolinic acid is chicken ovalbumin ( OVA), the invisible control blotting zone is made by blotting rabbit anti-mouse IgG antibody solution on cellulose membrane, the cellulose membrane layer is made of carboxylated cellulose membrane, and the protective film at the handle end covering the water-absorbing material layer is green. Both the blotting band and the stealth control blotting band are "┬".

[0055] Judgment of results: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means that the test result is positive, indicating that oxolinic acid is contained in the sample to be tested; (b) negative if there is a blot on the cellulose membrane There are two brown-red blots "┬" on the cellulose membrane, indicating that th...

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Abstract

The invention discloses a test strip for quickly detecting oxolinic acid and a preparation method thereof. A bottom layer of the test strip is a support layer; a middle layer is an adsorbed layer; a protecting layer is fixed on the adsorbed layer; the adsorbed layer is orderly divided into an adsorption fiber layer, a gold-labelled antibody fiber layer, a cellulose membrane layer and an absorbent material layer at the handle end from a test end; the gold-labelled antibody in the gold-labelled antibody fiber layer is an anti-oxolinic acid polyclonal antibody or monoclonal antibody labelled by colloidal gold; a detection imprinting and a contrast imprinting are arranged on the cellulose membrane layer; the detection imprinting is printed by carrier protein coupled to the oxolinic acid; the contrast imprinting is printed by a goat anti-rabbit or rabbit anti-mouse IgG antibody or a goat anti-rabbit IgG antibody. The test strip disclosed by the invention is simple in structure, strong in specificity, high in sensitivity and accuracy, simple and convenient to operate, wide in application range, low in price, and easy to popularize and apply, and has better social and economic value.

Description

technical field [0001] The invention belongs to the field of immunological detection of veterinary drug residues, in particular to an immune colloidal gold test strip for detecting oxolinic acid residues and a preparation method thereof. Background technique [0002] Oxolinic Acid (OA for short), also known as Oxolinic Acid, has the molecular formula C 13 h 11 NO 5 , with a molecular weight of 261.23, is a broad-spectrum antibacterial drug, especially has strong antibacterial activity against Gram-negative bacteria, and has a small dosage and good antibacterial effect. It is one of the ideal pesticides for treating aquatic animal diseases. Oxolinic acid is usually white or off-white crystalline powder, odorless and tasteless, insoluble in water and ethanol, soluble in formic acid and sodium hydroxide solution, non-hygroscopic, stable to light and heat. However, it has certain toxic and side effects on the human body. The common ones mainly include gastrointestinal reactio...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/5082G01N33/558G01N33/577G01N33/9446
Inventor 张改平王栋邢广旭贾国超王方雨胡骁飞杨继飞
Owner 河南百奥生物工程有限公司
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