Test strip for quickly detecting oxolinic acid and preparation method thereof
The technology of oxaquinic acid and test strips is applied in the field of immunological detection of veterinary drug residues, which can solve the problems of inability to conduct on-site detection in large-scale batch detection, affecting the accuracy of analysis results, and high requirements for instrument maintenance, achieving obvious economic benefits and Social benefits, saving expensive instruments and equipment costs, and low storage temperature requirements
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Embodiment 1
[0031] Example 1: Test strips and preparation methods for rapid detection of trace amounts of oxolinic acid, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic thin strips, the absorbent fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-oxquinic acid monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose The water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the junctions cross and penetrate each other. On the cellulose membrane layer 4, there are detection blots 6 and control blots 7, the detection blots are printed with bovine serum albumin (BSA) solution coupled with oxolinic acid, and the control blots are blotted with rabbit anti-mouse IgG antibody As a result, two imprints are arranged in parallel to for...
Embodiment 2
[0050] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the gold-labeled antibody fiber layer is adsorbed with polyclonal antibodies against oxquinic acid, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane layer is made of The pure cellulose membrane, the detection imprinted band and the invisible control imprinted band are all "ten", and the protective film at the handle end covering the water-absorbing material layer is blue.
[0051] The sample to be tested is chicken liver, the sample is cut into pieces, smashed, and then centrifuged at 4000r / min for 5min, and the upper layer juice is taken as the sample to be tested;
[0052] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that oxolinic acid is contained in the sample to be tested; (b) negative if there are two brown-red blo...
Embodiment 3
[0054] Embodiment 3: The structure of the test strip is basically the same as that of Embodiment 1, except that the absorbent fiber layer is made of polyvinylidene fluoride membrane (PVDF), and the carrier protein solution coupled with oxolinic acid is chicken ovalbumin ( OVA), the invisible control blotting zone is made by blotting rabbit anti-mouse IgG antibody solution on cellulose membrane, the cellulose membrane layer is made of carboxylated cellulose membrane, and the protective film at the handle end covering the water-absorbing material layer is green. Both the blotting band and the stealth control blotting band are "┬".
[0055] Judgment of results: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means that the test result is positive, indicating that oxolinic acid is contained in the sample to be tested; (b) negative if there is a blot on the cellulose membrane There are two brown-red blots "┬" on the cellulose membrane, indicating that th...
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