Immunochromatography test strip for detecting capsaicin in gutter oil and preparation method thereof
A technology of immunochromatographic test paper and capsaicin, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of limited application range, difficult promotion and application, complicated process, etc., and achieve high sensitivity, high sensitivity, and strong specificity Effect
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Embodiment 1
[0042] The immunochromatographic test paper of detection capsaicin of the present invention, with reference to Figure 1-2 , including a support body and an adsorption layer fixed on the support body. The adsorption layer consists of the adsorption fiber layer 1, the gold-labeled antibody fiber layer 2, the cellulose film layer 3, and the water-absorbing material layer 4 at the handle end from the test end. The film layer is provided with a stealth detection blot 5 printed with a carrier protein solution coupled with capsaicin, and a stealth control blot 6 printed with a goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solution; The gold-labeled antibody fiber layer is made of glass fiber cotton that absorbs gold-labeled antibodies, and the gold-labeled antibodies are capsaicin monoclonal or polyclonal antibodies labeled with nano-gold particles.
[0043] The support body includes a bottom layer 7 arranged on the bottom surface of the adsorption laye...
Embodiment 2
[0049] The immunochromatographic test strip for detecting capsaicin in this embodiment mainly includes: preparation of capsaicin carrier protein conjugate, preparation of capsaicin monoclonal antibody or polyclonal antibody, preparation of capsaicin gold-labeled antibody, and immunochromatography The steps such as the preparation of test strip, wherein, the preparation method of each product is as follows:
[0050] 1. Preparation of capsaicin carrier protein conjugate (artificially combined with antigen)
[0051] (1) Synthesis of capsaicin-HS by succinic anhydride method: Weigh 26g of capsaicin and 13g of succinic anhydride into a 500mL reaction flask, add 100mL of dimethyl sulfoxide (DMSO) as a solvent, and react at 90°C for 1h; Cool to room temperature, slowly add dropwise to 400mL distilled water, stir while adding dropwise, a large number of light yellow precipitates precipitate out of the solution; suction filter the precipitate, wash with ice distilled water and drain, d...
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