Immunochromatography test strip for detecting capsaicin in gutter oil and preparation method thereof

A technology of immunochromatographic test paper and capsaicin, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of limited application range, difficult promotion and application, complicated process, etc., and achieve high sensitivity, high sensitivity, and strong specificity Effect

Inactive Publication Date: 2018-12-11
山西公大智检科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods require expensive instruments and equipment, require skilled professionals to operate, the process is complicated, and the time is long, which limits its application range and is difficult to promote and apply in production.
[0004] The immunochromatographic detection method has semi-quantitative and certain quantitative capabilities, and can provide preliminary information on the analyte. This method has high sensitivity and relatively simple analysis process. It has unique advantages in the screening of capsaicin, and it needs to be developed first. detection technology, but the current commercial capsaicin immunochromatographic detection test strips are still blank in China, and urgently need to be researched and solved

Method used

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  • Immunochromatography test strip for detecting capsaicin in gutter oil and preparation method thereof
  • Immunochromatography test strip for detecting capsaicin in gutter oil and preparation method thereof
  • Immunochromatography test strip for detecting capsaicin in gutter oil and preparation method thereof

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Effect test

Embodiment 1

[0042] The immunochromatographic test paper of detection capsaicin of the present invention, with reference to Figure 1-2 , including a support body and an adsorption layer fixed on the support body. The adsorption layer consists of the adsorption fiber layer 1, the gold-labeled antibody fiber layer 2, the cellulose film layer 3, and the water-absorbing material layer 4 at the handle end from the test end. The film layer is provided with a stealth detection blot 5 printed with a carrier protein solution coupled with capsaicin, and a stealth control blot 6 printed with a goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solution; The gold-labeled antibody fiber layer is made of glass fiber cotton that absorbs gold-labeled antibodies, and the gold-labeled antibodies are capsaicin monoclonal or polyclonal antibodies labeled with nano-gold particles.

[0043] The support body includes a bottom layer 7 arranged on the bottom surface of the adsorption laye...

Embodiment 2

[0049] The immunochromatographic test strip for detecting capsaicin in this embodiment mainly includes: preparation of capsaicin carrier protein conjugate, preparation of capsaicin monoclonal antibody or polyclonal antibody, preparation of capsaicin gold-labeled antibody, and immunochromatography The steps such as the preparation of test strip, wherein, the preparation method of each product is as follows:

[0050] 1. Preparation of capsaicin carrier protein conjugate (artificially combined with antigen)

[0051] (1) Synthesis of capsaicin-HS by succinic anhydride method: Weigh 26g of capsaicin and 13g of succinic anhydride into a 500mL reaction flask, add 100mL of dimethyl sulfoxide (DMSO) as a solvent, and react at 90°C for 1h; Cool to room temperature, slowly add dropwise to 400mL distilled water, stir while adding dropwise, a large number of light yellow precipitates precipitate out of the solution; suction filter the precipitate, wash with ice distilled water and drain, d...

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Abstract

The invention discloses an immunochromatography test strip for detecting capsaicin in gutter oil and a preparation method thereof. The immunochromatography test strip comprises a supporter and an adsorption layer, wherein the adsorption layer is fixedly arranged on the supporter; an adsorption fiber layer, a gold label antibody fiber layer, a cellulose membrane layer and a water-absorbing materiallayer are sequentially arranged on the adsorption layer from the testing end; the cellulose membrane layer is provided with a hidden detection imprint which is printed by a capsaicin-coupled carrierprotein solution, and a hidden contrast imprint which is printed by goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solutions; the gold label antibody fiber layer is made of glass fiber cotton adsorbed with gold label antibody, and the gold label antibody is nanogold particle-labeled capsaicin monoclonal antibody or multiclonal antibody. The immunochromatography test strip has the advantages that the specificity is strong; the sensitivity is high, and 1.7ng/ml of capsaicin can be detected; the operation is simple and convenient, the detection is quick, the detectionresult can be judged within 5min, the result display is visualized, intuitive and accurate, the application range is wide, and the immunochromatography test strip is suitable for being popularized and applied.

Description

technical field [0001] The invention relates to an immunochromatographic test paper, in particular to an immunochromatographic test paper which uses gold-labeled nanoparticles to mark capsaicin antibodies and a preparation method thereof. Background technique [0002] In recent years, with the continuous improvement of people's living standards, people's demand and consumption of edible oil have been increasing, and the waste oil produced has also increased significantly. Many criminals collect swill oil, old frying oil, pig and beef offal oil, etc., and after refining and processing, the crime of illegally selling gutter oil as edible oil has occurred frequently in recent years. It not only damages the interests of consumers, interferes with the management order of waste oil in our country, but also poses a huge threat to the health of the people. Due to eating habits and taste preferences, chili is one of the most widely used and widely used condiments. Capsaicin is the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 任飞冯斌刘庆华刘斌
Owner 山西公大智检科技有限公司
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