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Test strip for rapidly detecting lincomycin residues and preparation method of test strip

A technology for detecting lincomycin and test paper, which is applied in the field of detection equipment for lincomycin residues, and achieves the effects of easy promotion, high labeling rate and strong specificity

Inactive Publication Date: 2012-12-12
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method not only requires expensive instruments and equipment and complex and cumbersome operation process, but also requires high skills of inspectors, and is not suitable for on-site monitoring and screening of a large number of samples

Method used

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  • Test strip for rapidly detecting lincomycin residues and preparation method of test strip
  • Test strip for rapidly detecting lincomycin residues and preparation method of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: see figure 1 , figure 2 . In the figure, 1 is the support layer, made of plastic sheet strips, 2 is the adsorption fiber layer (test end), made of glass fiber cotton, and the gold-labeled antibody fiber layer 3 is made of gold-labeled antibody glass fiber cotton that absorbs lincomycin The gold-labeled antibody is a colloidal gold-labeled anti-lincomycin monoclonal antibody.

[0036] The cellulose membrane layer 4 is made of nitrocellulose membrane, the water-absorbing material layer 5 is made of water-absorbing filter paper, and the absorbent fiber layer 2, the gold-labeled antibody fiber layer 3, the cellulose membrane layer 4, and the water-absorbing material layer 5 are arranged from right to left Paste on the support layer 1 in turn, and the fibers at the junctions cross and penetrate each other. On the cellulose membrane layer 4, there are detection blots 6 printed with LIN-coupled carrier protein BSA (bovine serum albumin) solution and contro...

Embodiment 2

[0055] Embodiment two: the test strip structure is basically the same as embodiment one, the difference is:

[0056] The support layer 1 is made of non-absorbent hard paper strips, the absorbent fiber layer 2 is made of nylon membrane, the gold-labeled antibody fiber layer 3 is adsorbed with colloidal gold-labeled anti-lincomycin polyclonal antibody, and the cellulose membrane layer 4 is made of pure For the cellulose membrane, the carrier protein coupled with LIN in detection blot 6 was chicken ovalbumin (OVA), and the control blot 7 was prepared on the cellulose membrane with rabbit anti-mouse IgG solution.

Embodiment 3

[0057] Embodiment three: the test strip structure is basically the same as embodiment one, the difference is:

[0058] Polyvinylidene fluoride (PVDF) membrane was used for adsorption fiber layer 2, carboxylated cellulose membrane was used for cellulose membrane layer 4, chicken ovalbumin (OVA) was used as the carrier protein coupled with LIN in detection blot 6, and chicken ovalbumin (OVA) was used for control blot 7 Goat anti-mouse IgG solutions were prepared on cellulose membranes.

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Abstract

The invention relates to a test strip for rapidly detecting residues and a preparation method of the test strip. The bottom layer of the test strip is used as a supporting layer; an intermediate adsorption layer is fixed on the supporting layer; an external protection layer is fixed on the adsorption layer; the adsorption layer comprises an adsorption fiber layer, a gold-labeled antibody fiber layer for adsorbing and coupling lincomycin and a cellulose membrane layer sequentially arranged on the testing sample end and further comprises a water absorbing material layer arranged on the handle end; a detection blot printed by a lincomycin carried protein solution and a control blot printed by a goat anti- or rabbit anti-mouse IgG antibody solution are arranged on the cellulose membrane layer. The test strip is high in detection specificity, high in sensitivity and simple and fast in operation; detection results are displayed visually, directly and accurately; a special apparatus and a professional are not in need in the detection; and the test strip has the following characteristics of low cost, low investment as well as easiness for promotion and application.

Description

technical field [0001] The invention relates to a detection tool for lincomycin residues, in particular to a test strip for rapid detection of lincomycin residues and a preparation method thereof. Background technique [0002] Lincomycin (lincomycin, LIN), also known as lincomycin, is a lincosamide antibiotic produced by the fermentation of Streptomyces lincoln, molecular formula: C18H34N2O6S, molecular weight: 406.53, has strong antibacterial activity, and mainly acts on positive bacteria, is an RNA-dependent acetylcholinesterase inhibitor. LIN does not have outstanding toxicity, but its residues in food and drugs are easy to accumulate after entering the human body through the food chain, which can lead to drug resistance of bacteria and lead to a lack of treatment efficiency for diseases. Therefore, all countries have limited requirements for lincomycin. The Ministry of Agriculture of China stipulates the maximum residue limit of LIN in cattle, sheep, pigs, and poultry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 胡骁飞张改平邢广旭职爱民王方雨张小辉杨继飞
Owner HENAN ACAD OF AGRI SCI
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