84 results about "Lincomycine" patented technology

The invention relates to a norhalal-enoxacin conjugate as well as a preparation method and application thereof. The norhalal-enoxacin conjugate comprises a norhalal-enoxacin coupling compound, or a stereoisomer, a prodrug, a crystal form, a pharmaceutically acceptable salt, a pharmaceutically acceptable ester or a pharmaceutically acceptable solvate selected from the compound. Compared with enoxacin, kanamycin, streptomycin, lincomycin and azithromycin, the norharmann-enoxacin conjugate disclosed by the invention has a smaller MIC (Minimum Inhibitory Concentration) value, which shows that the norharmann-enoxacin conjugate has a better anti-staphylococcus aureus effect.

The invention discloses a culture medium for improving the fermentation titer of lincomycin and a culture method, and relates to the field of microbial fermentation. Lincomycin streptomycete is taken as a starting strain, biological nitrogen is added into seeds and a fermentation culture medium basic material, and biological nitrogen and a trace element TE are added into a supplementary material, so that an obvious regulating effect on promoting carbon and nitrogen source metabolism in a fermentation process is achieved, and proper conditions are provided for thallus growth and element production; by adjusting the proportion of biological nitrogen, soybean meal, ammonium sulfate and corn steep liquor supplemented into the mixed nitrogen material in the process, the maintainability of thalli is further improved, and fermentation production of lincomycin is facilitated. The method is suitable for stably and efficiently producing lincomycin on a large scale, and has the advantages of simplicity in operation, remarkable optimization effect and easiness in popularization.

The invention relates to the technical field of genetic engineering, and provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG6736 gene, the SLCG6736 gene in the streptomyces lincomycin is deleted through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and the obtained strain is used for fermentation production of lincomycin. Wherein the nucleotide sequence of the SLCG6736 gene is as shown in SEQ ID NO. 1. The invention further provides application of the method. The lincomycin biosynthesis negative regulator SLCG6736 has the advantages that the lincomycin biosynthesis negative regulator SLCG6736 is screened in the research, the SLCG6736 gene is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the lincomycin fermentation yield in industrial production.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3904 gene, which comprises the following steps: deleting a GlnR family transcriptional regulation gene SLCG3904 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3904 gene is as shown in SEQ ID NO. 1. The invention further provides application of the method. The invention has the advantages that the lincomycin biosynthesis negative regulator SLCG3904 (GlnR family) is screened in the research, the SLCG3904 gene on the streptomyces lincomycin chromosome is deleted through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the lincomycin fermentation yield in industrial production.

The invention discloses a process for repairing damaged soil ecology by lincomycin waste liquid fertilizer, and relates to the technical field of soil remediation, which comprises the following specific steps: step 1, screening and culturing microbial decomposition flora, the invention has the following advantages: through the chelation of the polyaspartic acid derivative on metal ions and the promotion effect on nutrient absorption, the release and transmission of nutrients are precisely regulated and controlled, so that crops can absorb and utilize the nutrients more efficiently; the nano composite material and the specific biological stimulant containing various beneficial microbial strains and natural plant extracts are jointly fused into the liquid fertilizer, the microbial strains in the specific biological stimulant activate the soil biological environment, the natural plant extracts assist plant growth to resist diseases, and the nano composite material and the natural plant extracts have a synergistic effect, so that the liquid fertilizer has a good application prospect. Physicochemical properties and biological activity of soil are optimized, excellent soil micro-ecology is created for plant growth, healthy and strong growth of plants is promoted, and restoration of damaged soil is enhanced.

The invention belongs to the technical field of genetic engineering, and particularly relates to a method for increasing the yield of lincomycin by knocking out a streptomyces lincomycin pAgene0296 gene. The method comprises the following steps: knocking out a coding gene pAgene0296 of TetR / AcrR family repressor protein in streptomyces lincomycin, and fermenting the obtained mutant strain to produce lincomycin. Experimental results show that after the pAgene0296 gene of the streptomyces lincomycin is knocked out, the yield of the lincomycin is remarkably increased. The invention further provides a construction method of the pAgene0296 gene deletion type streptomyces lincoli mutant strain, according to the method, the coding gene of the streptomyces lincoli is directionally modified through a gene engineering technology, so that the pAgene0296 gene is knocked out, and the obtained pAgene0296 gene deletion type streptomyces lincoli mutant strain can be used for efficiently producing lincomycin or a product in the synthesis process of the lincomycin.

The invention provides a preparation method of clindamycin hydrochloride alcoholate. The preparation method comprises the following steps: firstly, preparing a chlorination reagent from an oily solvent, DMF (Dimethyl Formamide), a chlorination solvent and an antioxidant; carrying out temperature-controlled chlorination reaction with lincomycin hydrochloride to obtain a chlorination reaction solution; adjusting the pH value of the reaction solution to 10-11, controlling the temperature to react, taking an oil phase after the reaction is finished, concentrating under reduced pressure, diluting with water, and adjusting the pH value to 3-5 to obtain a column loading solution; the preparation method comprises the following steps: dissolving clindamycin hydrochloride in water, adsorbing by using resin, eluting impurities by using dilute alkaline water, analyzing by using ethanol and deionized water in sequence, collecting an analysis solution, concentrating under reduced pressure, adding ethanol, heating for dissolving, adjusting the pH value to 1-1.5, cooling for crystallizing, and drying to obtain the clindamycin hydrochloride alcoholate. According to the preparation method disclosed by the invention, the clindamycin alcoholate with high yield and high purity is prepared by pertinently designing processes such as reaction conditions, a specific oily reagent, temperature control conditions, column adsorption, dilute alkaline water impurity elution, ethanol desorption and the like, only one-time crystallization is needed, repeated refining is not needed, the HPLC (High Performance Liquid Chromatography) purity is up to 99.5% or above, and the clindamycin alcoholate is suitable for industrial production. And 0.14% or less of 2, 7-epiclindamycin.

The invention relates to a compound bath product containing lincomycin and vitamin B6 and a preparation method of the compound bath product, and relates to the technical field of daily chemical products. Aiming at the problems of single effect, low component residence rate, poor medical component adaptability and the like of a traditional bath product in acne care, a triple synergistic formula of lincomycin (antibacterial), vitamin B6 (oil control) and glycyrrhizic acid / glycosylglycerol (anti-inflammatory) is innovatively constructed, and the adjuvant therapy effect on mild acne is effectively improved through clinical verification; a pH / temperature dual-response microcapsule wrapping technology is developed, so that the transdermal absorption rate of active ingredients is effectively improved, and the washing loss rate is less than or equal to 15%; the traditional Chinese medicine composition breaks through the limitation of medical cream types, is matched with bath carriers such as facial cleanser, shampoo, soap, shower gel and the like, and realizes daily application of acne care components. The product disclosed by the invention has the advantages of controlling oil within 6 hours and reducing the skin irritation by 42%, and fills up the technical blank of washing and caring products in medicine-grade efficacy and use convenience.

The invention relates to the technical field of sheep semen storage, and discloses a sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid, the sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid comprises a basic diluent and an additive, the additive is indole-3-pyruvic acid, and the concentration of the additive in the diluent is 15 mg / L-35 mg / L. The basic diluent comprises the following components: 3.1 g of glucose; 1.4 g of sodium citrate; 20 mL of yolk; 100 [mu] L of lincomycin hydrochloride; 0.50 g of bovine serum albumin; and 0.50 g of vitamin C. The preparation method of the basic diluent comprises the following steps: dissolving glucose, sodium citrate, bovine serum albumin and vitamin C in distilled water, oscillating, filtering and sterilizing, adding yolk and lincomycin hydrochloride, uniformly mixing to obtain the basic diluent, and sealing and storing at 4 DEG C. According to the sheep fresh sperm low-temperature preservation diluent containing indole-3-pyruvic acid provided by the invention, 25 mg / L of indole-3-pyruvic acid is added into the diluent, so that the preservation quality and time efficiency of sheep sperms under the low-temperature condition of 4 DEG C can be improved.

The invention belongs to the field of liquid fertilizers, and particularly relates to a method for promoting bright colors of flowers by using a lincomycin finished product liquid fertilizer, which comprises the following steps: diluting lincomycin dry powder according to a mass ratio of 1: 100 to obtain a lincomycin finished product liquid; the method comprises the following steps: carrying out aerobic culture in a liquid culture medium containing starch, yeast powder, soybean meal, yeast extract and inorganic salt, supplementing ammonium ions in the lincomycin fermentation process, and controlling the fermentation time to be 36-110 hours, so as to obtain lincomycin fermentation liquor; dissolving urea, potassium chloride, zinc sulfate and the iron fulvic acid solution in water, and uniformly mixing to prepare a nutrient solution; the method comprises the following steps: treating zeolite with the fineness of 200 meshes at a high temperature of 200 DEG C; airing or drying the zeolite until the water content of the zeolite is reduced to be not higher than 5%; according to the lincomycin finished product liquid fertilizer disclosed by the invention, the flowering period is effectively prolonged, so that the bright color effect of flowers is promoted.

The invention relates to a high-throughput screening method of high-yield lincomycin strains, and discloses a rapid lincomycin detection method which comprises the following steps: adding a proper amount of oxalic acid into a detection sample under the condition of a phosphate buffer solution, and calculating the yield of lincomycin according to an absorbance value obtained by detection. By combining porous plate culture and shake flask fermentation re-screening of streptomyces lincomycin, high-throughput screening of high-yield lincomycin strains is realized. Compared with the traditional screening method, the high-throughput screening method provided by the invention has the advantages of rapidness, high efficiency, simplicity, feasibility and the like, the screening period can be shortened, the screening efficiency of the strain is improved, the workload of breeding the high-yield lincomycin strain is greatly reduced, and a powerful technical guarantee is provided for breeding the high-yield strain.

The invention belongs to the field of liquid fertilizers, and relates to a process for promoting the growth of commercial crops by using a lincomycin finished product liquid fertilizer, which comprises the following steps: diluting lincomycin dry powder according to a mass ratio of 1: 100 to obtain a lincomycin finished product liquid; the fertilizer is prepared from a modified accelerant, urea, monopotassium phosphate, calcium superphosphate, a calcium magnesium phosphate fertilizer, potassium chloride, zinc sulfate, borax, an iron fulvate solution, paclobutrazol and lincomycin fermentation liquor. Dissolving urea, potassium chloride, zinc sulfate and the iron fulvic acid solution in water, and uniformly mixing to prepare a nutrient solution; performing 200-DEG C high-temperature treatment on zeolite with the fineness of 200 meshes, and spraying the nutrient solution onto the zeolite; airing or drying the zeolite until the water content of the zeolite is reduced to be not higher than 5%; and uniformly mixing the residual chemical fertilizer with the modified accelerant to obtain the lincomycin finished product liquid fertilizer. The lincomycin finished product liquid fertilizer provided by the invention can improve the growth of crops.

The application belongs to the field of electrochemical detection, and particularly relates to a water-vapor self-powered sensor for lincomycin detection and a preparation method thereof. A glass substrate is used as a substrate, a copper foil tape is used as a positive electrode and a negative electrode, a ZnO nanowire array and Ce-MOF are used as power generation materials to prepare a water-vapor generator, and a lincomycin aptamer is assembled to construct a light-assisted water-vapor self-powered sensor. The power generation material is a composite material of the ZnO nanowire array and the Ce-MOF. The light assistance is combined into the water-vapor self-powered sensor, and the water-vapor self-powered sensor significantly improves the generated electric output under the light assistance. The application widens the application of the ZnO nanowire array and the Ce-MOF in water-vapor induced power generation, and provides a new strategy for electrochemical detection. The constructed light-assisted water-vapor self-powered sensor has a wide detection range, high sensitivity, low detection limit and low detection cost.

The present invention provides a method for increasing the yield of lincomycin by modifying the SLCG_2951 gene of Streptomyces lincomycin, knocking out the SLCG_2951 gene of Streptomyces lincomycin by genetic engineering, obtaining a high-yield strain of lincomycin, and fermenting and producing lincomycin A with the obtained strain; wherein the nucleotide sequence of the SLCG_2951 gene is shown in SEQ ID NO.1. The present invention also provides an application of the above method. The present invention screens the SLCG_2951 gene that affects the mycelium morphology, knocks out the SLCG_2951 gene on the genome of Streptomyces lincomycin by genetic engineering, and can delay the mycelium breakage of Streptomyces lincomycin A134 on a fermentation tank, thereby increasing the yield of lincomycin A, and providing technical support for increasing the yield of lincomycin in industrial production.

The utility model discloses a lincomycin hydrochloride injection clarity detection device which comprises a machine body, control bins and a bottom bin, a lamp body is installed in the machine body, the control bins are arranged on the two sides in the machine body, rotating motors are installed in the control bins, the output ends of the rotating motors are connected with lead screws in a fastening mode, and the lead screws are connected with the bottom bin in a fastening mode. The surface of the screw rod is in threaded connection with a sliding sleeve, one side of the sliding sleeve is connected with a connecting frame, camera shooting equipment is installed on the connecting frame, a bottom bin is arranged at the bottom of the machine body, a frame body is placed in the bottom bin, and a fixing block and a connecting plate are connected to the frame body. The lead screw is matched with the sliding sleeve to drive the camera shooting equipment on the connecting frame to descend, so that injection is shot, the flexibility of the camera shooting equipment is improved, picture information is transmitted to the PLC through the signal transmitter, the PLC compares and records the picture information, and therefore foreign matter can be found quickly, and the detection efficiency is improved.

The invention relates to a compound external lotion based on lincomycin, which is characterized in that every two or three of lincomycin, lidocaine and vitamin B6 are compounded to form a multi-effect synergistic local treatment composition. The lotion takes an antibacterial effect as a core, integrates the functions of easing pain, relieving itching, resisting inflammation and repairing, and can synchronously solve the problems of skin infection, pain and barrier injury. Lincomycin efficiently inhibits pathogenic microorganisms, lidocaine quickly relieves pain and itching symptoms, vitamin B6 promotes epidermis repair and metabolic regulation, the lincomycin, lidocaine and vitamin B6 are scientifically matched to achieve complementary synergism, and every two of the lincomycin, lidocaine and vitamin B6 are simplified and combined to meet different clinical requirements. The dosage form design covers solution, gel and suspension dosage forms, has permeability, adhesiveness and stability, and is suitable for various skin lesions such as acute exudation and chronic wound surfaces. According to the scheme, the systemic side effects are reduced through local administration, and the treatment safety and the patient compliance are remarkably improved through optimization of low-irritation auxiliary materials.

The invention discloses a method for separating lincomycin A from fermentation liquor based on turbine extraction-reversed-phase polymer chromatography cooperation, and belongs to the technical field of biotechnology abstract. The method specifically comprises the following steps: step (1), carrying out multistage counter-current extraction on the lincomycin A fermentation liquor by adopting a turbine extraction technology, and large-scale rapid enrichment and extraction of lincomycin A are realized. And (2) directly carrying out reversed-phase polymer chromatography (filler is a styrene polymer) on a concentrated solution after turbine extraction, and carrying out gradient elution to realize efficient separation of lincomycin A and impurities. Through the connection of turbine extraction and chromatographic separation technologies, the production efficiency is effectively improved, the labor cost is reduced, the production energy consumption is reduced, the yield and the purity are improved, the production treatment capacity is high, and the method is suitable for large-scale industrial production.

The application discloses a method for reducing lincomycin B component in a lincomycin fermentation process, which comprises adding soybean protein hydrolysate A in the lincomycin fermentation process, so that the lincomycin B component can be effectively reduced, and the A component titer can be improved. The soybean protein hydrolysate A is a complex amino acid mixture obtained by enzymolysis of soybean raw materials, and the components include peptide segment A, free amino acid solution A, water or others, wherein the mass percentage of the peptide segment A is 30-35%, the mass percentage of the free amino acid solution A is 55-65%, and the water and the others account for 5-15%. After optimization, the production level of the B component in a 50 L fermenter is at least 166 mu g / mL, which is reduced by more than 75% compared with the yield of the B component in the original fermentation culture method, the product quality and the production efficiency are improved, and the method is suitable for industrialized scale production.

The invention discloses a three-waste cooperative treatment system in a lincomycin production process, and relates to the technical field of treatment of waste gas, waste water and waste residues generated in an antibiotic production process, and the three-waste cooperative treatment system comprises a waste gas treatment subsystem, a waste water treatment subsystem, a waste residue treatment subsystem and a terminal treatment subsystem; the waste gas treatment subsystem comprises two waste gas treatment routes, namely a waste gas adsorption and concentration treatment subsystem and a sewage aeration treatment subsystem; the wastewater treatment subsystem comprises procedures of pretreatment, hydrolytic acidification, a 1 # mid-sedimentation tank, an anaerobic section, a 2 # mid-sedimentation tank, an aerobic section, a sedimentation tank, a mixed reaction tank and a final sedimentation tank which are connected in sequence. According to the three-waste cooperative treatment system, harmlessness and recycling of three-waste treatment of waste gas, waste water and waste residues are achieved, heat sources of different qualities are provided for the three-waste treatment system through the terminal system, maximum energy utilization is achieved, and therefore energy consumption is reduced, pollutant near zero emission is achieved, and up-to-standard emission in the true sense is achieved.

The invention provides a method for continuously producing clindamycin hydrochloride, which comprises the following steps of: 1, preparing a solution of triphosgene in DMF (Dimethyl Formamide), namely a solution A; 2, preparing a solution of lincomycin or hydrochloride thereof in chloroform, namely a solution B; step 3, pumping the solution A and the solution B into a reaction plate according to a certain flow velocity for reaction to obtain a reaction solution C; step 4, pumping the reaction liquid C into a reaction plate according to a certain flow velocity for reaction to obtain a reaction liquid D; 5, the reaction liquid D is subjected to aftertreatment, and clindamycin hydrochloride is obtained. The method is high in reaction conversion rate, few in side reaction impurities, safe in production and suitable for industrial production.

The invention discloses a method for detecting the content of an impurity E and an impurity F. Liquid chromatography-mass spectrometry (LC-MS / MS) is adopted for detection, and the method comprises the following steps: preparing a reference substance solution, preparing a test solution, and performing liquid chromatography-mass spectrometry (LC-MS / MS) detection. Carrying out liquid chromatography-mass spectrometry (LC-MS / MS) detection: injecting the reference substance solution and the test solution into a liquid chromatography-mass spectrometer, and carrying out separation detection; the parameter conditions of the liquid chromatography are as follows: separation is performed by adopting a mobile phase gradient elution method, and the mobile phase gradient elution process is as follows: when the time is 0-2 minutes, the mobile phase A accounts for 65-75%, and the mobile phase B accounts for 25-35%. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3615 gene, which comprises the following steps: knocking out a TetR family transcriptional regulation gene SLCG3615 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3615 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3615 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG3615 is screened in the research, the SLCG3615 gene copy on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

The invention belongs to the field of liquid fertilizers, and particularly relates to a method for preparing a liquid fertilizer from lincomycin waste liquid to improve the yield of food crops, which comprises the following steps: step 1, fermentation treatment: obtaining a lincomycin dry product; step 2, fermenting: fermenting for 36-110 hours, and purifying lincomycin from fermentation liquor by using an extraction reagent formed by mixing methanol and ethyl acetate after fermentation is completed; 3, synthesis: adjusting the pH values of the nutrient solution A, the nutrient solution B, the nutrient solution C, the nutrient solution D and the lincomycin to 5.8-6.2 to obtain the liquid fertilizer prepared from the lincomycin waste liquid. The liquid fertilizer prepared from the lincomycin waste liquid can effectively improve the yield of crops.

This invention discloses a method for detecting impurities E and F in lincomycin hydrochloride injection, using liquid chromatography-mass spectrometry (LC-MS / MS). The method includes the following steps: preparing a reference solution, preparing a test solution, and LC-MS / MS detection. The LC-MS / MS detection involves injecting the reference solution and the test solution into the LC-MS / MS instrument for separation and detection. The LC parameters are as follows: separation is performed using a gradient elution method, with mobile phase A at 65%–75% and mobile phase B at 25%–35% during the 0–2 min period. This method is simple to operate, fast in detection, and has the advantages of high sensitivity, high precision, and high repeatability.

The invention discloses a method for reducing impurity content in clindamycin hydrochloride alcoholate, which comprises the following steps: 1) preparing a clindamycin free alkali solution by using lincomycin hydrochloride, adding water into the clindamycin free alkali solution, then dropwise adding hydrochloric acid to adjust the pH value, and layering to obtain a clindamycin hydrochloride aqueous solution; 2) adjusting the pH value of the obtained clindamycin hydrochloride aqueous solution to 5-7; then adding monopotassium phosphate and dipotassium phosphate, stirring, dissolving and clarifying, heating, and cooling; and 3) adding ethanol and sodium salt, heating, stirring, dissolving, cooling, preserving heat, crystallizing, cooling to below 10 DEG C, and centrifuging to obtain the clindamycin hydrochloride alcoholate. According to the method, the content of 7-clindamycin is remarkably reduced, meanwhile, the problem that clindamycin hydrochloride is degraded to generate lincomycin, 7-epilincomycin impurities and the like can be effectively inhibited, and the content of related impurities is effectively controlled and remarkably reduced; and the related treatment method is simple, convenient to operate and suitable for industrial production.

The invention discloses a method for detecting the content of chloromethane in a lincomycin hydrochloride bulk drug. According to the detection method disclosed by the invention, a to-be-detected sample is detected by adopting gas chromatography-mass spectrometry (GC-MS); the parameter conditions of the gas chromatography are as follows: the temperature of a sample inlet is 160-180 DEG C; the heating procedure is as follows: the initial temperature is 35-45 DEG C, the temperature is raised to 230-250 DEG C at the heating rate of 45-55 DEG C / min, and the temperature is kept for 0 min; the parameter conditions of the mass spectrum are as follows: the acquisition type is SIM; the ion source is EI; the solvent delay is 0.2 to 0.8 min; the temperature of the transmission line is 230-250 DEG C; the temperature of a quadrupole rod is 140-160 DEG C; and the temperature of the ion source is 220-240 DEG C. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention discloses a method for detecting the content of chlorobutane in a lincomycin hydrochloride bulk drug. The method comprises the following steps: detecting a sample to be detected by adopting gas chromatography-mass spectrometry (GC-MS); the parameter conditions of the gas chromatography are as follows: the temperature of a sample inlet is 145-155 DEG C; the heating procedure is as follows: the initial temperature is 65-75 DEG C, the temperature is kept for 1.5-2.5 min, and the temperature is raised to 95-105 DEG C at the heating rate of 8-12 DEG C / min; the temperature is raised to 235-245 DEG C at the temperature raising speed of 65-75 DEG C / min, and the temperature is kept for 3-5 min; the parameter conditions of the mass spectrum are as follows: the acquisition type is MRM; the ion source is EI; the solvent delay is 1.5 to 2.5 min; the temperature of the transmission line is 245-255 DEG C; the temperature of a quadrupole rod is 145-155 DEG C; and the temperature of the ion source is 225-235 DEG C. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_1540 Methods to increase lincomycin production through gene therapy involve knocking out LacI family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_1540 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_1540 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides the above-mentioned... SLCG_1540 Applications of gene knockout modified *Streptomyces lincomycin*. The advantages of this invention are: the screening of a negative regulator of lincomycin biosynthesis, SLCG_1540, and experimental findings that gene knockout of *Streptomyces lincomycin* chromosomes via genetic engineering... SLCG_1540 This invention provides technical support for increasing lincomycin fermentation yield in industrial production by generating genes that can produce high-yield strains of lincomycin.