118 results about "Lincomycine" patented technology

The invention provides an extraction method of lincomycin. The extraction method mainly comprises the following steps: adjusting acidity of fermentation liquor, filtering by using a ceramic ultrafiltration membrane, then adjusting alkalinity, then filtering by using a ceramic ultrafiltration membrane again, then carrying out primary sec-octyl alcohol extraction and hydrochloric acid back-extraction, adjusting alkalinity of the primary back extraction liquor and then filtering by using a ceramic ultrafiltration membrane again; carrying out secondary sec-octyl alcohol extraction and hydrochloric acid back-extraction and adding acetone to the secondary back extraction liquor to carry out evaporative crystallization. According to the extraction method provided by the invention, the yield of the lincomycin is high, the color grade of the lincomycin can be improved, and the B component content in the lincomycin is reduced. According to the extraction method of the lincomycin, the feed liquid quality of other extraction technologies can be improved by a membrane treatment process, the integral extraction efficiency is further improved, the residual volume of sec-octyl in raffinate in the extraction procedure is reduced, the product quality is improved, and meanwhile, the COD (Chemical Oxygen Demand) in produced wastewater is reduced. The extraction method has certain economic benefit and environmental benefit.

The invention relates to a lincomycin hydrochloride extraction method which comprises the following process steps: performing acidification pre-treatment on lincomycin fermentation broth, dual aqueous phase extraction, butanol extraction, hydrochloric acid reextraction, activated carbon decoloring, acetone crystallization, and drying, thereby obtaining a solid product lincomycin hydrochloride. Due to the adopted dual aqueous phase extraction technique for extract lincomycin hydrochloride, lincomycin can be effectively enriched, the use amount of butanol can be reduced, the solvent consumption can be reduced, dual aqueous phase raw materials used in the method are free of environment pollution, lincomycin can be produced in a large scale, economic and environment-friendly requirements can be met, and positive contribution can be made.

The invention discloses a lincomycin and spectinomycin compound oil suspension injection and a preparation method and application thereof. The injection comprises a mixed auxiliary material solvent, lincomycin hydrochloride and spectinomycin hydrochloride. The mixed auxiliary material solvent system comprises oil or ester for injection, suspending agent, emulsifier, wetting agent and antioxidant. The preparation method comprises adding suspending agent into oil or ester for injection to obtain gel solvent system, sequentially adding emulsifier, wetting agent and antioxidant, adding lincomycin hydrochloride and spectinomycin hydrochloride, and grinding with a colloidal mill. The invention can effectively improve the stability of spectinomycin and reduce the irritation on animal body during usage, and has simple preparation process, strong operability and high value of popularization.

The invention discloses a lactobacillus plantarum specific culture medium. The culture medium is convenient to prepare, is low in cost, and contains substances of lincomycin and a carbon source, wherein after cooperative use of lincomycin and the carbon source, the culture medium can be used for culturing lactobacillus plantarums and counting specificity in fermented milk, lactobacillus beverages or bacterial powder. The invention also provides an application of using the lactobacillus plantarum specific culture medium which is used for counting viable lactobacillus plantarums by culturing strains in the culture medium and then performing plate counting on cultured strains; the counting method is simple, is high in specificity, and relative to a PCR counting method, is lower in requirement on operators and lower in cost. The lactobacillus plantarum specific culture medium disclosed by the invention is suitable for preparing a culture medium capable of performing specificity counting on viable lactobacillus plantarums.

The invention discloses a pharmaceutical composition for curing chronic nasosinusitis and a preparation method thereof. The pharmaceutical composition contains phlegm-resolving prescription ambroxol hydrochloride and antibiotics which is one of Beta- lactam, aminoglycoside, macrolide, carbostyril, anti-anaerobe and lincomycin, or mixture of one of the antibiotics except the anti-anaerobe and the anti-anaerobe. The composition adopts a sinuses irrigation method to administrate and has the characteristics of convenient use, good validity, high safety and hard recurrence for the cure of the acute nasosinusitis and the chronic nasosinusitis.

The invention provides lincomycin-spectinomycin compound nano-emulsion, and belongs to the technical field of pharmacy. The compound nano-emulsion is prepared from the following components in parts by weight: 1-20 parts of lincomycin hydrochloride, 1-25 parts of spectinomycin, 15-40 parts of surfactant, 1-25 parts of co-surfactant, 1-16 parts of oil and 18-75 parts of water. The compound nano-emulsion is a novel preparation which is combined with characteristics of lincomycin hydrochloride and lincomycin hydrochloride, and has the advantages of uniform grain diameter distribution, good fluidity, high stability, good permeability and good bioavailability; the compound nano-emulsion can be quickly phagocytosed by reticuloendothelial cells after being taken orally, drugs can quickly take effects, and constant plasma concentration and pharmacological effect can be maintained, the bioavailability can be improved, drug effect can be enhanced, and using amount and taking times of the medicine can be reduced.

The invention discloses an extraction method of lincomycin. A lincomycin fermentation solution is filtered through a ceramic film with pore diameters ranging from 0.01 mu m to 0.1 mu m after the pH (potential of hydrogen) of the lincomycin fermentation solution is adjusted to 2.0-4.5, then an acid aqueous solution with the pH ranging from 2.0 to 4.0 is used for washing the ceramic film repeatedly, filter liquor is discarded, a hydrochloric acid aqueous solution with the pH ranging from 8 to 10 is used for washing the ceramic film repeatedly, purified water is added for dissolution, and a lincomcin aqueous solution is obtained. Clear filter liquor is obtained after filtration of the ceramic film, a destaining solution and a desorbed solution can be both used for production of the next batch of lincomycin, the utilization rate of raw materials can be increased, meanwhile, the ultimate yield of the next batch of products can be further increased, and the extraction method has the advantages that a precipitation method is simple and convenient and a technological process is applicable to large-scale production.

The invention discloses a spectinomycin hydrochloride lincomycin hydrochloride soluble powder which can be miscible with an oil emulsion vaccine after being dissolved in water, and belongs to the technical field of veterinary medicine preparations. The composition of the spectinomycin hydrochloride lincomycin hydrochloride soluble powder which can be miscible with an oil emulsion vaccine after being dissolved in water is as follows: 40 parts of spectinomycin hydrochloride, 20 parts of lincomycin hydrochloride, 2-4 parts of an emulsifier, 1.5-2.5 parts of a dispersant, 5-10 parts of a buffer, 0.5-1.0 part of a stabilizer, and 22.5-31 parts of an auxiliary material. After being dissolved in water, the soluble powder of the invention does not require any oil emulsion vaccine dilution solutionand can realize uniform mixing with an oil emulsion vaccine. The problems in the prior art that an oil emulsion vaccine dilution solution is needed for dissolution, the dissolution effect is poor, uniformity is low, a biological utilization rate is low, the use cost is relatively high, and the like are solved. On the basis of ensuring the quality and curative effects of such products, the clinical operation and application of breeding is facilitated, and the use cost is reduced.

The invention provides an acne removing preparation with no side effect and good curative effect. The preparation comprises the following components in percentage by weight: 28-56 percent of ketoconazole and clobetasol propionate cream, 20-28 percent of lincomycin hydrochloride gel, 8-20 percent moroxydine hydrochloride, 4-10 percent of metronidazole and 10-40 percent of traditional Chinese medicinal extract, wherein the traditional Chinese medicinal extract comprises extract of white peony root, white poria, bighead atractylodes rhizome and angelica root. The acne removing preparation is effectively combined with traditional Chinese medicines and western medicines, can be used for quickly removing pox and acne, and has the effect of whitening.

The invention discloses genetically engineered bacteria for producing lincomycin. The genetically engineered bacteria are constructed by knocking out a gene for encoding protein containing a tetratricopeptide repeat (TPR) structural domain of streptomyces lincolnensis, knocking out a gene for encoding an RNA enzyme and performing overexpression on a gene for encoding Xre family protein of the streptomyces lincolnensis. The invention also discloses a construction method of the genetically engineered bacteria for producing the lincomycin. The method comprises the steps of knocking out the gene for encoding the protein containing the TPR structural domain of the streptomyces lincolnensis by using the streptomyces lincolnensis as a starting strain, knocking out the gene for encoding the RNA enzyme and performing overexpression on the gene for encoding the Xre family protein of the streptomyces lincolnensis to construct the genetically engineered bacteria for producing the lincomycin. The construction method of the genetically engineered bacteria for producing the lincomycin provided by the invention is easy and convenient to operate, and the obtained genetically engineered bacteria canincrease the yield of the lincomycin and have good application prospects.

The invention discloses a compound kanamycin sulfate injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces the time and dosage of taking drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 billion units of kanamycin monosulfate, 1 to 10kg of lincomycin hydrochloride, 0.1 to 2kg of doxofylline, 0.05kg of dexamethasone sodium phosphate, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 10kg of dimethyl acetamide and the balance of water for injection. The injection liquid reasonably mixes usage of all the components by a plurality of types of drugs, has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound kanamycin sulfate injection liquid has convenient use, and can achieve the treatment effect of a plurality of types of drugs.

The invention discloses an anti-toxoplasma composition drug and a screening method thereof, and belongs to the field of medicine. According to the anti-toxoplasma composition drug and the screening method thereof, mouse test toxoplasmosis animal models are established, the ten drugs of a sulfadiazine sodium injection, a compound sulfamethoxazole, a lincomycin hydrochloride injection, a sulfamonomethoxine sodium injection, a florfenicol injection, acetylspiramycin, pyrimethamine, roxithromycin, artemisinin and radix sophorae flavescentis are selected out, two compositions which have the best anti-toxoplasma effect are screened out, one composition comprises the sulfamonomethoxine, the pyrimethamine and TMP, and the other composition comprises the compound sulfamethoxazole and the acetylspiramycin; animal models in mice and pigs are established, it is verified that the two compositions are both effective on the treatment of artificially infected toxplasmosis in pigs, and the treatment effect on the pigs is in accordance with that of the mouse models. By means of the anti-toxoplasma composition drug and the screening method thereof, it is proved that the the feasible method is achieved by utilizing the mouse test toxoplasmosis models to evaluate the efficacy of drugs on treating toxplasmosis in pigs, and meanwhile two anti-toxoplasma drug compositions with good effects are provided.

The invention discloses a method for improving the yield of lincomycin through streptomyces lincolnensis regulation gene combination transformation. Strains are obtained by knocking out an Lrp familytranscription regulatory gene SLCG-4846 and a TetR family transcription regulatory gene SLCG-2919 from streptomyces lincolnensis in a combined manner. Meanwhile, the invention further discloses the method for improving the yield of lincomycin through streptomyces lincolnensis regulation gene combination modification. According to the method, traceless knockout is sequentially carried out on a plurality of genes through a homologous recombination technology, and resistance genes are not introduced in the process, so that the fermentation cost is reduced, and the hereditary stability is guaranteed. Meanwhile, the process does not influence the growth of thalli, and most importantly, multiple knockout experiments can be continuously carried out in the same strain. According to the method, theyield of lincomycin can be greatly increased, and a new technical support is provided for improving the yield of lincomycin in industrial production.

The invention belongs to the technical field of functional material preparation, and more specifically relates to a preparation method and applications of click chemistry based lincomycin molecularlyimprinted composite membrane. The preparation method comprises following steps: dopamine and polyethyleneimine are taken as hydrophilic modification materials, Lincomycin is taken as template molecule, 4-vinylpyridine is taken as a functional monomer, Pentaerythritol Tetra(3-mercaptopropionate) is taken as a crosslinking agent, Dipentaerythritol penta-/hexa-acrylate is taken as an auxiliary crosslinking agent, based on click chemistry polymerization method, the lincomycin molecule composite film is prepared. The prepared click chemistry based lincomycin molecularly imprinted composite membraneis capable of solving problems in the prior art that recycling of conventional lincomycin molecule imprinting polymer is difficult, and secondary pollution is easily caused; and in addition, the click chemistry based lincomycin molecularly imprinted composite membrane possesses excellent specific recognition capacity and adsorption separation capacity on Lincomycin.

The invention discloses a method for recovering lincomycin from waste active carbon decolorized by lincomycin. The method comprises the following steps: washing decolorized waste active carbon by neutral or acid saturated butanol water or extracted waste liquor containing 5-10% butanol liquid or alkalization liquid and filtering, regulating pH of the filtrate by NaOH solution, and extracting and refining by butanol to recover lincomycin from decolorized waste active carbon. The method can help recover lincomycin from the waste active carbon decolorized by lincomycin, increase yield of lincomycin, and has great economical benefits and social benefits.

The invention discloses macroporous adsorption resin for lincomycin extraction and a synthesis method thereof. The method comprises the following steps: stirring and dissolving in a suspension polymerization manner to prepare a water phase; dissolving a monomer, an initiator and a pore-foaming agent together, and stirring until the components are completely dissolved to prepare an oil phase; putting an oil phase into a water phase, standing until the oil phase and the water phase are completely layered, heating, and keeping the temperature; after the reaction is finished, putting the resin into a steam generator for extraction, recovering the pore-foaming agent, washing with water, and drying to obtain a dry polystyrene divinylbenzene copolymer; and adding the prepared dry polystyrene divinylbenzene copolymer into a reaction kettle, swelling with dichloroethane, adding a catalyst in twice, heating to react, washing the resin with water until the resin is neutral after the reaction is finished, discharging, and screening the macroporous adsorption resin to obtain the macroporous adsorption resin for lincomycin extraction. The macroporous adsorption resin prepared by the method not only has high adsorption capacity and high desorption rate on lincomycin, but also is simple in synthesis process, green, environment-friendly, low in cost and easy for large-scale production.

The invention discloses lincomycin hydrochloride gel and a preparation process thereof. Lincomycin hydrochloride and lidocaine hydrochloride are used as main drugs of the gel, oleic macrogolglyceride is used as a permeation enhancer, caprylic capric triglyceride is used as a surfactant, and octanoate decanoate macrogolglyceride is used as an oil solvent. The preparation process of the preparation disclosed by the invention is simple, no special storage condition is needed, the product stability is good, and more importantly, the transdermal permeability of the lidocaine hydrochloride is improved, so as to further improve the efficacy.

The invention belongs to veterinary insect repellent medicine and preparing method technical field, and in particular relates to a decoquinate and lincomycin hydrochloride-containing pet insect repellent tablet and a preparation method thereof, the pet insect repellent tablet preparation comprises (a) decoquinate and lincomycin hydrochloride as raw material medicines, (b) an adhesive, (c) a wetting agent, (d) a disintegrating agent, (e) a filler and (f) a sweetener. The decoquinate and lincomycin hydrochloride-containing pet insect repellent tablet is prepared by a wet process, and is a white-like tablet in character. The decoquinate and lincomycin hydrochloride-containing pet insect repellent tablet is good in stability and good in palatability, and can be used a veterinary oral preparation.

The invention discloses a lincomycin hydrochloride oil solution and a preparation method and application thereof, and belongs to the field of medicine and pharmacy. The lincomycin hydrochloride oil solution overcomes the problem of the easy settlement of a mixed suspend lincomycin hydrochloride oil preparation. The lincomycin hydrochloride oil solution is simple in preparation process only needing common physical mixing without grinding process. The lincomycin hydrochloride oil solution is free of visible insolubles under a microscope, and can be sterilized through a 0.22 mum millipore filter film. The lincomycin hydrochloride oil solution product has good liquidity, and may not be hung on the wall, the appearance is single-phase, transparent and clear, clarity detection is acceptable, and after repeated freezing and thawing, preparation stratification and drug settlement phenomenon may not occur. The lincomycin hydrochloride oil solution has the advantages of simple process, stable quality and great application prospect. The lincomycin hydrochloride oil solution product is used for treatment of mastitis of lactating cows infected with staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus uberis escherichia coli and fungus and the like.

The invention relates to a multi-component carbon nitride-based non-metallic material, a preparation method and application thereof, the method comprises the following steps: step 1: dissolving graphene oxide in a urea aqueous solution, adding a reducing agent to carry out a reduction reaction to obtain a precursor containing urea and reduced graphene oxide; and 2, uniformly mixing the precursor,carbon quantum dots and oxalic acid, and carrying out high-temperature calcination to obtain the multi-element carbon nitride-based non-metallic material. The multi-element carbon nitride-based non-metallic material prepared by the invention has the effects of absorbing light in a wide area (the absorption edge exceeds 605nm), promoting the generation of superoxide radicals (. O2 < ->) of active species and accelerating the separation of photon-generated carriers. Through the synergistic effect, the CD-rGO-O-g/C3N4 has very high photocatalytic activity on lincomycin. The method is simple in synthesis process flow and high in operability, and has a wide application prospect.

The invention relates to a preparation method of imprinted polymers of core-shell type molecules, products of the imprinted polymers of the core-shell type molecules, and the application of the products. The preparation method comprises the following steps: synthesizing a seed emulsion by adopting a monomeric compound shown as a formula II and a monomeric compound shown as a formula III; using the seed emulsion as a core, adopting the monomeric compound shown as the formula I and the monomeric compound shown as the formula II to carry out random copolymerization with the seed emulsion so as to obtain the imprinted polymers of the core-shell type molecules. According to the preparation method of the imprinted polymers of the core-shell type molecules, the products of the imprinted polymers of the core-shell type molecules, and the application of the products, which are disclosed by the invention, can be used for extracting lincomycin A with higher purity from lincomycin and analogues of the lincomycin; under the water-phase condition, or in a water solution, the lincomycin A, the lincomycin B and other analogous ingredient components can be separated, so that the lincomycin A with higher purity is obtained; a better separation result can be obtained when the preparation method is combined with the columns chromatography.