49 results about "Lincomycine" patented technology

The invention relates to a norhalal-enoxacin conjugate as well as a preparation method and application thereof. The norhalal-enoxacin conjugate comprises a norhalal-enoxacin coupling compound, or a stereoisomer, a prodrug, a crystal form, a pharmaceutically acceptable salt, a pharmaceutically acceptable ester or a pharmaceutically acceptable solvate selected from the compound. Compared with enoxacin, kanamycin, streptomycin, lincomycin and azithromycin, the norharmann-enoxacin conjugate disclosed by the invention has a smaller MIC (Minimum Inhibitory Concentration) value, which shows that the norharmann-enoxacin conjugate has a better anti-staphylococcus aureus effect.

The invention provides a preparation method of clindamycin hydrochloride alcoholate. The preparation method comprises the following steps: firstly, preparing a chlorination reagent from an oily solvent, DMF (Dimethyl Formamide), a chlorination solvent and an antioxidant; carrying out temperature-controlled chlorination reaction with lincomycin hydrochloride to obtain a chlorination reaction solution; adjusting the pH value of the reaction solution to 10-11, controlling the temperature to react, taking an oil phase after the reaction is finished, concentrating under reduced pressure, diluting with water, and adjusting the pH value to 3-5 to obtain a column loading solution; the preparation method comprises the following steps: dissolving clindamycin hydrochloride in water, adsorbing by using resin, eluting impurities by using dilute alkaline water, analyzing by using ethanol and deionized water in sequence, collecting an analysis solution, concentrating under reduced pressure, adding ethanol, heating for dissolving, adjusting the pH value to 1-1.5, cooling for crystallizing, and drying to obtain the clindamycin hydrochloride alcoholate. According to the preparation method disclosed by the invention, the clindamycin alcoholate with high yield and high purity is prepared by pertinently designing processes such as reaction conditions, a specific oily reagent, temperature control conditions, column adsorption, dilute alkaline water impurity elution, ethanol desorption and the like, only one-time crystallization is needed, repeated refining is not needed, the HPLC (High Performance Liquid Chromatography) purity is up to 99.5% or above, and the clindamycin alcoholate is suitable for industrial production. And 0.14% or less of 2, 7-epiclindamycin.

The invention relates to the technical field of sheep semen storage, and discloses a sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid, the sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid comprises a basic diluent and an additive, the additive is indole-3-pyruvic acid, and the concentration of the additive in the diluent is 15 mg / L-35 mg / L. The basic diluent comprises the following components: 3.1 g of glucose; 1.4 g of sodium citrate; 20 mL of yolk; 100 [mu] L of lincomycin hydrochloride; 0.50 g of bovine serum albumin; and 0.50 g of vitamin C. The preparation method of the basic diluent comprises the following steps: dissolving glucose, sodium citrate, bovine serum albumin and vitamin C in distilled water, oscillating, filtering and sterilizing, adding yolk and lincomycin hydrochloride, uniformly mixing to obtain the basic diluent, and sealing and storing at 4 DEG C. According to the sheep fresh sperm low-temperature preservation diluent containing indole-3-pyruvic acid provided by the invention, 25 mg / L of indole-3-pyruvic acid is added into the diluent, so that the preservation quality and time efficiency of sheep sperms under the low-temperature condition of 4 DEG C can be improved.

The application belongs to the field of electrochemical detection, and particularly relates to a water-vapor self-powered sensor for lincomycin detection and a preparation method thereof. A glass substrate is used as a substrate, a copper foil tape is used as a positive electrode and a negative electrode, a ZnO nanowire array and Ce-MOF are used as power generation materials to prepare a water-vapor generator, and a lincomycin aptamer is assembled to construct a light-assisted water-vapor self-powered sensor. The power generation material is a composite material of the ZnO nanowire array and the Ce-MOF. The light assistance is combined into the water-vapor self-powered sensor, and the water-vapor self-powered sensor significantly improves the generated electric output under the light assistance. The application widens the application of the ZnO nanowire array and the Ce-MOF in water-vapor induced power generation, and provides a new strategy for electrochemical detection. The constructed light-assisted water-vapor self-powered sensor has a wide detection range, high sensitivity, low detection limit and low detection cost.

The invention relates to a compound external lotion based on lincomycin, which is characterized in that every two or three of lincomycin, lidocaine and vitamin B6 are compounded to form a multi-effect synergistic local treatment composition. The lotion takes an antibacterial effect as a core, integrates the functions of easing pain, relieving itching, resisting inflammation and repairing, and can synchronously solve the problems of skin infection, pain and barrier injury. Lincomycin efficiently inhibits pathogenic microorganisms, lidocaine quickly relieves pain and itching symptoms, vitamin B6 promotes epidermis repair and metabolic regulation, the lincomycin, lidocaine and vitamin B6 are scientifically matched to achieve complementary synergism, and every two of the lincomycin, lidocaine and vitamin B6 are simplified and combined to meet different clinical requirements. The dosage form design covers solution, gel and suspension dosage forms, has permeability, adhesiveness and stability, and is suitable for various skin lesions such as acute exudation and chronic wound surfaces. According to the scheme, the systemic side effects are reduced through local administration, and the treatment safety and the patient compliance are remarkably improved through optimization of low-irritation auxiliary materials.

The invention discloses a method for separating lincomycin A from fermentation liquor based on turbine extraction-reversed-phase polymer chromatography cooperation, and belongs to the technical field of biotechnology abstract. The method specifically comprises the following steps: step (1), carrying out multistage counter-current extraction on the lincomycin A fermentation liquor by adopting a turbine extraction technology, and large-scale rapid enrichment and extraction of lincomycin A are realized. And (2) directly carrying out reversed-phase polymer chromatography (filler is a styrene polymer) on a concentrated solution after turbine extraction, and carrying out gradient elution to realize efficient separation of lincomycin A and impurities. Through the connection of turbine extraction and chromatographic separation technologies, the production efficiency is effectively improved, the labor cost is reduced, the production energy consumption is reduced, the yield and the purity are improved, the production treatment capacity is high, and the method is suitable for large-scale industrial production.

The application discloses a method for reducing lincomycin B component in a lincomycin fermentation process, which comprises adding soybean protein hydrolysate A in the lincomycin fermentation process, so that the lincomycin B component can be effectively reduced, and the A component titer can be improved. The soybean protein hydrolysate A is a complex amino acid mixture obtained by enzymolysis of soybean raw materials, and the components include peptide segment A, free amino acid solution A, water or others, wherein the mass percentage of the peptide segment A is 30-35%, the mass percentage of the free amino acid solution A is 55-65%, and the water and the others account for 5-15%. After optimization, the production level of the B component in a 50 L fermenter is at least 166 mu g / mL, which is reduced by more than 75% compared with the yield of the B component in the original fermentation culture method, the product quality and the production efficiency are improved, and the method is suitable for industrialized scale production.

The invention discloses a method for detecting the content of an impurity E and an impurity F. Liquid chromatography-mass spectrometry (LC-MS / MS) is adopted for detection, and the method comprises the following steps: preparing a reference substance solution, preparing a test solution, and performing liquid chromatography-mass spectrometry (LC-MS / MS) detection. Carrying out liquid chromatography-mass spectrometry (LC-MS / MS) detection: injecting the reference substance solution and the test solution into a liquid chromatography-mass spectrometer, and carrying out separation detection; the parameter conditions of the liquid chromatography are as follows: separation is performed by adopting a mobile phase gradient elution method, and the mobile phase gradient elution process is as follows: when the time is 0-2 minutes, the mobile phase A accounts for 65-75%, and the mobile phase B accounts for 25-35%. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3615 gene, which comprises the following steps: knocking out a TetR family transcriptional regulation gene SLCG3615 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3615 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3615 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG3615 is screened in the research, the SLCG3615 gene copy on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

This invention discloses a method for detecting impurities E and F in lincomycin hydrochloride injection, using liquid chromatography-mass spectrometry (LC-MS / MS). The method includes the following steps: preparing a reference solution, preparing a test solution, and LC-MS / MS detection. The LC-MS / MS detection involves injecting the reference solution and the test solution into the LC-MS / MS instrument for separation and detection. The LC parameters are as follows: separation is performed using a gradient elution method, with mobile phase A at 65%–75% and mobile phase B at 25%–35% during the 0–2 min period. This method is simple to operate, fast in detection, and has the advantages of high sensitivity, high precision, and high repeatability.

The invention discloses a method for detecting the content of chloromethane in a lincomycin hydrochloride bulk drug. According to the detection method disclosed by the invention, a to-be-detected sample is detected by adopting gas chromatography-mass spectrometry (GC-MS); the parameter conditions of the gas chromatography are as follows: the temperature of a sample inlet is 160-180 DEG C; the heating procedure is as follows: the initial temperature is 35-45 DEG C, the temperature is raised to 230-250 DEG C at the heating rate of 45-55 DEG C / min, and the temperature is kept for 0 min; the parameter conditions of the mass spectrum are as follows: the acquisition type is SIM; the ion source is EI; the solvent delay is 0.2 to 0.8 min; the temperature of the transmission line is 230-250 DEG C; the temperature of a quadrupole rod is 140-160 DEG C; and the temperature of the ion source is 220-240 DEG C. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention discloses a method for detecting the content of chlorobutane in a lincomycin hydrochloride bulk drug. The method comprises the following steps: detecting a sample to be detected by adopting gas chromatography-mass spectrometry (GC-MS); the parameter conditions of the gas chromatography are as follows: the temperature of a sample inlet is 145-155 DEG C; the heating procedure is as follows: the initial temperature is 65-75 DEG C, the temperature is kept for 1.5-2.5 min, and the temperature is raised to 95-105 DEG C at the heating rate of 8-12 DEG C / min; the temperature is raised to 235-245 DEG C at the temperature raising speed of 65-75 DEG C / min, and the temperature is kept for 3-5 min; the parameter conditions of the mass spectrum are as follows: the acquisition type is MRM; the ion source is EI; the solvent delay is 1.5 to 2.5 min; the temperature of the transmission line is 245-255 DEG C; the temperature of a quadrupole rod is 145-155 DEG C; and the temperature of the ion source is 225-235 DEG C. The method disclosed by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision and high repeatability.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_1540 Methods to increase lincomycin production through gene therapy involve knocking out LacI family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_1540 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_1540 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides the above-mentioned... SLCG_1540 Applications of gene knockout modified *Streptomyces lincomycin*. The advantages of this invention are: the screening of a negative regulator of lincomycin biosynthesis, SLCG_1540, and experimental findings that gene knockout of *Streptomyces lincomycin* chromosomes via genetic engineering... SLCG_1540 This invention provides technical support for increasing lincomycin fermentation yield in industrial production by generating genes that can produce high-yield strains of lincomycin.

The invention discloses a rapid lincomycin detection method based on surface enhanced Raman spectroscopy. The rapid lincomycin detection method comprises the following steps: preparing silver sol containing coarse silver nanoparticles with the average particle size of about 50nm; putting the silver sol containing the rough silver nanoparticles into a centrifugal machine to be treated, forming supernate containing silver nanoparticle scraps and reaction impurities on the upper layer, removing the supernate according to the proportion that the supernate accounts for 80-95% of the total volume of the centrifuged liquid, and then putting the residual sedimentation part into an ultrasonic cleaning machine to be subjected to ultrasonic dispersion, the silver nanoparticles with the uniform particle size are obtained and serve as an SERS enhanced substrate; mixing the SERS enhanced substrate with a lincomycin sample according to a volume ratio of 1: 1-3: 1 to obtain a detection mixed solution; performing SERS (Surface Enhanced Raman Scattering) detection on the detection mixed solution under set detection conditions to obtain a mixed solution SERS spectrum; extracting a characteristic Raman peak from the SERS spectrum of the mixed solution; and based on the extracted lincomycin characteristic Raman peak, carrying out modeling analysis of a regression algorithm so as to carry out quantitative detection of lincomycin.

According to the detection method for the lincomycin hydrochloride related substances, one or more related substances in a lincomycin hydrochloride sample are detected by adopting a high performance liquid chromatography-mass spectrometry method, and the related substances in the lincomycin hydrochloride can be simply, rapidly and accurately detected by selecting specific chromatographic conditions. Therefore, the quality controllability of the lincomycin hydrochloride and the preparation thereof is ensured.

1.The name of the design product: packaging box (lincomycin lidocaine gel). 2.The use of the design product: for medicine packaging. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

This invention relates to the field of immunology, and more particularly to a hybridoma cell line and its secreted monoclonal antibody against the metamizole residue marker 4-methylaminoantipyrine (MAA), and its applications. This invention uses 4-aminoantipyrine to react with methyl 4-bromomethylbenzoate, followed by alkaline hydrolysis to obtain a hapten (Formula I). ​​An immunogen is then synthesized and used to immunize mice. After cell fusion and screening, a hybridoma cell line capable of secreting a monoclonal antibody recognizing MAA is prepared, named Metamizole-6A4. This hybridoma cell line has a high efficiency in secreting monoclonal antibodies. The secreted monoclonal antibody can accurately detect MAA, IC50, and other markers. 50 The concentration is 4.06 ng / mL, with high specificity. Cross-reactivity with aminopyrine and its metabolites 4-aminoantipyrine, 4-formamidoantipyrine, 4-acetamidoantipyrine, sulfadiazine, norfloxacin, lincomycin, tylosin, penicillin G, streptomycin, and gentamicin is less than 5%. It can accurately detect MAA residues in food. Formula I.

The present application relates to a preparation method of clindamycin hydrochloride impurities, and belongs to the field of medicines.The technical problem to be solved by the present application is that there is a lack of a method for efficiently preparing 7-epiclinamycin and 7-epiclinamycin hydrochloride reference substances of clindamycin hydrochloride impurities in the prior art.The present application provides a preparation method of a clindamycin hydrochloride impurity intermediate shown in formula II: the intermediate is prepared from a compound shown in formula I and R1COOH through a Mitsunobu substitution reaction, and amine and / or a nitrogen-containing aromatic heterocyclic compound are added to the reaction solution.The present application further provides a complete synthesis method of 7-epiclinamycin and 7-epiclinamycin hydrochloride: starting from lincomycin, the method comprises the following steps: protecting a group on silicon, selectively deprotecting, Mitsunobu substitution reaction, hydrolysis reaction to obtain 7-epiclinamycin, and further chlorination reaction to obtain 7-epiclinamycin hydrochloride.

The application discloses a compound soluble powder of spectinomycin hydrochloride and lincomycin hydrochloride and a preparation method thereof. Compared with the prior art, the compound soluble powder of spectinomycin hydrochloride and lincomycin hydrochloride prepared by the application has high affinity to drugs, is safe to use, significantly improves the drug dissolution speed and stability of the drug after dissolution in water and oil environments, overcomes the problems of insufficient drug dissolution and irritation of the drug to a drug administration site, reduces the hemolytic side effect of the drug for animals and the nephrotoxicity, and can also reduce the drug resistance of the drug for animals by inhibiting the function of P-sugar protein. The compound soluble powder of spectinomycin hydrochloride and lincomycin hydrochloride can expand the antibacterial spectrum and simultaneously prevent and treat various diseases by the synergistic effect of spectinomycin and lincomycin.

The invention relates to a method for determining related substances in a lincomycin hydrochloride injection, which is characterized in that a high performance liquid chromatography is adopted, an ion pair reagent is used in a mobile phase, and related impurities in the lincomycin hydrochloride injection are separated from lincomycin; the related impurities are one or more of an impurity A, an impurity B, an impurity C, an impurity F, an impurity G, an impurity H and lincomycin B. The determination method disclosed by the invention is high in sensitivity, strong in specificity, good in accuracy and simple, convenient and rapid to operate, perfects the quality standard of determination of the related substances in the lincomycin hydrochloride injection, can strictly control the quality of the related substances in the lincomycin hydrochloride injection, ensures the safety and reliability of the lincomycin hydrochloride injection, and has a wide application prospect. The practical significance is realized.

The invention belongs to the technical field of biological medicines, and discloses application of lincomycin in preparation of a medicine for treating melanoma. The lincomycin disclosed by the invention has the capability of remarkably weakening proliferation, migration and invasion of melanoma cells, shows an obvious tumor inhibition effect in a mouse transplantation tumor model, and effectively delays tumor growth. Therefore, the lincomycin provided by the invention is used as a novel candidate drug in melanoma treatment, and a safe and effective treatment choice with reutilization value is provided for clinic.

The present application relates to the technical field of drug synthesis, and particularly relates to a preparation method of clindamycin phosphate intermediate. The preparation method of the clindamycin phosphate intermediate provided by the present application takes hydrochloric acid lincomycin and Vilsmeier reagent as starting materials, and precisely controls the material ratio through a micro-mixer and a micro-channel reactor, so that the stereochemical selectivity of the chlorination step is significantly improved, the reaction rate is effectively improved, the generation of 7-epi-clindamycin impurities is reduced through continuous preparation, the subsequent purification cost is reduced, the control of 7-epi-clindamycin impurities is realized, the 7-epi-clindamycin impurities can be completely removed after one-time crystallization of the product, and the yield reaches 95-98%.

This invention relates to the field of genetic engineering technology and provides a method for increasing lincomycin production by modifying the SLCG_1608 gene in *Streptomyces lincomyces*. The method involves overexpressing the Lrp family transcriptional regulatory gene SLCG_1608 in *Streptomyces lincomyces* through genetic engineering, obtaining a high-yielding lincomycin-producing strain, which is then used for fermentation to produce lincomycin. The nucleotide sequence of the SLCG_1608 gene is shown in SEQ ID NO.1. This invention also provides the application of the above method in industrial strains. The advantages of this invention are: increasing the copy number of the SLCG_1608 gene on the chromosome of *Streptomyces lincomyces* through genetic engineering, obtaining a high-yielding lincomycin-producing strain, and providing technical support for increasing the fermentation yield of lincomycin in industrial production.

The present invention relates to a method for rapidly detecting lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water, belonging to the technical field of analytical detection. By means of specific fully automated on-line solid-phase extraction conditions in combination with specific high performance liquid chromatography-tandem mass spectrometry conditions, the present invention realizes sample enrichment, purification, separation and determination, and fully automatically analyzes lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water. The present invention realizes fully automated operation, and the total determination time only needs 11 min. The detection limit reaches the ng / L level. By washing the solid-phase extraction column with an ammonium bicarbonate aqueous solution, the problem of matrix effect commonly encountered in the analysis of complex matrix samples is overcome, meeting the requirements for ultra-trace detection of lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water.

The invention relates to the technical field of genetic engineering, and provides a method for improving the yield of lincomycin by modifying a streptomyces lincolensis SLCG3264 gene, a MarR family transcriptional regulation gene SLCG3264 in streptomyces lincolensis is knocked out through a genetic engineering way to obtain a lincomycin high-yield strain, and the obtained strain is used for fermentation production of lincomycin. Wherein the nucleotide sequence of the SLCG3264 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3264 gene knockout modified streptomyces lincomus. According to the invention, the lincomycin biosynthesis negative regulator SLCG3264 is screened, and the SLCG3264 gene on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, so that a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_3615 A method to increase lincomycin production through gene therapy involves knocking out the TetR family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_3615 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_3615 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides... SLCG_3615 Applications of gene knockout modified *Streptomyces lincomycin*. In this study, the negative regulator of lincomycin biosynthesis, SLCG_3615, was screened and knocked out on the chromosome of *Streptomyces lincomycin* via genetic engineering. SLCG_3615 SLCG_3615 Gene copying can yield high-yield strains of lincomycin, providing technical support for increasing the fermentation yield of lincomycin in industrial production.

The application relates to an extraction method of lincomycin hydrochloride, which comprises the following steps: S1, solid-liquid separation of lincomycin fermentation liquor after alkalization to obtain an alkalized liquor; S2, extraction of the alkalized liquor with a low-carbon alcohol to obtain an extraction liquor; S3, impurity removal and solid-liquid separation of the extraction liquor through a polyamide-ODS reverse-phase silica gel column to obtain a lincomycin separation liquor; and S4, decolorization of the lincomycin separation liquor with activated carbon, acidification and nanofiltration concentration in sequence. Through the low-carbon alcohol extraction, the polyamide-ODS reverse-phase silica gel column separation and the flocculation impurity removal technology, the technical problem that the content of group A is high and the content of group B is low in the lincomycin hydrochloride extraction and separation process is effectively solved, the lincomycin hydrochloride purification process is optimized, and the product quality of the lincomycin hydrochloride is improved.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG1540 gene, which comprises the following steps: knocking out a LacI family transcriptional regulation gene SLCG1540 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG1540 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG1540 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG1540 has the advantages that the lincomycin biosynthesis negative regulator SLCG1540 is screened out, and it is found through experiments that a lincomycin high-yield strain can be obtained by deleting the SLCG1540 gene on a streptomyces lincomycin chromosome through a genetic engineering way; the invention provides technical support for increasing the fermentation yield of lincomycin in industrial production.