2 results about "Lincomycin-B" patented technology

The application discloses a method for reducing lincomycin B component in a lincomycin fermentation process, which comprises adding soybean protein hydrolysate A in the lincomycin fermentation process, so that the lincomycin B component can be effectively reduced, and the A component titer can be improved. The soybean protein hydrolysate A is a complex amino acid mixture obtained by enzymolysis of soybean raw materials, and the components include peptide segment A, free amino acid solution A, water or others, wherein the mass percentage of the peptide segment A is 30-35%, the mass percentage of the free amino acid solution A is 55-65%, and the water and the others account for 5-15%. After optimization, the production level of the B component in a 50 L fermenter is at least 166 mu g / mL, which is reduced by more than 75% compared with the yield of the B component in the original fermentation culture method, the product quality and the production efficiency are improved, and the method is suitable for industrialized scale production.

The invention relates to a method for determining related substances in a lincomycin hydrochloride injection, which is characterized in that a high performance liquid chromatography is adopted, an ion pair reagent is used in a mobile phase, and related impurities in the lincomycin hydrochloride injection are separated from lincomycin; the related impurities are one or more of an impurity A, an impurity B, an impurity C, an impurity F, an impurity G, an impurity H and lincomycin B. The determination method disclosed by the invention is high in sensitivity, strong in specificity, good in accuracy and simple, convenient and rapid to operate, perfects the quality standard of determination of the related substances in the lincomycin hydrochloride injection, can strictly control the quality of the related substances in the lincomycin hydrochloride injection, ensures the safety and reliability of the lincomycin hydrochloride injection, and has a wide application prospect. The practical significance is realized.