Chromogenic culture medium for separating and detecting shigella

A chromogenic medium, Shigella technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problem of not being able to distinguish between Salmonella and Shigella, and difficult to detect Shigella at the same time Bacteria and black hearts are not obvious, to reduce false positive and false negative results, simple result judgment, and less colony color

Active Publication Date: 2012-10-03
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, this medium cannot distinguish between Salmonella and Shigella; on the other hand, other bacteria that do not produce β-galactosidase will not be able to distinguish from Shigella, resulting in false positives
Although there are currently Shigella chromogenic media that use Salmonella to produce hydrogen sulfide to show black hearts on the medium to distinguish Salmonella, but Salmonella that do not produce hydrogen sulfide can still not be distinguished from Shigella, and some produce hydrogen sulfide Salmonella in the culture medium produces black centers that are not obvious and can also cause false positive results
[0005] To sum up, the current technical bottleneck of the medium used for isolation and detection of Shigella is: on the one hand, it is difficult to detect four species of Shigella at the same time in one medium, resulting in missed detection; On the one hand, it is difficult to distinguish Shigella from Enterobacteriaceae such as Salmonella, resulting in false positive results

Method used

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  • Chromogenic culture medium for separating and detecting shigella
  • Chromogenic culture medium for separating and detecting shigella
  • Chromogenic culture medium for separating and detecting shigella

Examples

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Embodiment 1

[0036]A chromogenic medium for detection and isolation of Shigella, each 1000 mL medium contains 4 g of yeast powder, 3 g of tryptone, 6 g of soybean peptone, 5 g of sodium chloride, 5 g of lactose, and 5 g of sucrose g, 2-deoxy-D-ribose 5 g, agar powder 15 g, 5-bromo-4-chloro-3-indole-β-glucoside 0.4 g, 5-bromo-4-chloro-3-indole- β-fucopyranoside 0.2 g, 5-bromo-4-chloro-3-indole-N-acetyl-β-galactoside 0.4 g, phenol red 0.08 g, No. 3 bile salt 6 g, novobiocin Sodium 4 mg, cefsulodin 9 mg, cefixime 0.03 mg, the balance is deionized water, pH 6.8±0.2.

Embodiment 2

[0038] A chromogenic medium for detection and isolation of Shigella, each 1000 mL medium contains 6 g of yeast powder, 3 g of tryptone, 7 g of soybean peptone, 5 g of sodium chloride, 3 g of lactose, and 8 g of sucrose g, 2-deoxy-D-ribose 7 g, agar powder 18 g, 5-bromo-4-chloro-3-indole-β-glucoside 0.2 g, 5-bromo-4-chloro-3-indole- β-fucopyranoside 0.1 g, 5-bromo-4-chloro-3-indole-N-acetyl-β-galactoside 0.2 g, phenol red 0.06 g, bile salt No. 3 5 g, novobiocin Sodium 3 mg, cefsulodin 3 mg, cefixime 0.05 mg, the balance is deionized water, pH 6.8±0.2.

Embodiment 3

[0040] A chromogenic medium for detection and isolation of Shigella, each 1000 mL medium contains 8 g of yeast powder, 2 g of tryptone, 6 g of soybean peptone, 5 g of sodium chloride, 5 g of lactose, and 5 g of sucrose g, 2-deoxy-D-ribose 10 g, agar powder 15 g, 5-bromo-4-chloro-3-indole-β-glucoside 0.1 g, 5-bromo-4-chloro-3-indole- β-fucopyranoside 0.2 g, 5-bromo-4-chloro-3-indole-N-acetyl-β-galactoside 0.4 g, phenol red 0.04 g, bile salt No. 3 7 g, novobiocin Sodium 2 mg, cefsulodin 8 mg, cefixime 0.07 mg, the balance is deionized water, pH 6.8±0.2.

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Abstract

The invention discloses a chromogenic culture medium for separating and detecting shigella; the culture medium comprises yeast powder, tryptone, soy peptone, sodium chloride, lactose, cane sugar, 2-deoxy-D-ribose, agar powder, beta-glucosaccharase chromogenic substrate, beta-pyranfucosidase chromogenic substrate, beta-galactosidase chromogenic substrate, phenol red, 3# cholate, novobiocin sodium salt, cefsulodin and cefixime; residue is water; the chromogenic culture medium provided by the invention can be used for separating and detecting four species of shigella and has advantages of high specificity, high sensitivity, easy operation, simple result judgment and the like; the chromogenic culture medium is suitable for detecting each sample and has wide application prospect; and detecting effect of the chromogenic culture medium can achieve level of like products in an import R&F company and is superior to inland like products.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for separating and detecting Shigella. Background technique [0002] Shigella (Shigella) belongs to Enterobacteriaceae, there are four species, namely Shigella dysenteriae ( Shigella dysenteriae ), Shigella flexneri ( Shigella flexneri ), Shigella baumannii ( Shigellaboydii ), Shigella sonnei ( Shigella Sonnei ). All four species of Shigella can produce ulcerative colonic infection of bacillary dysentery in humans, and are the most important pathogenic bacteria in bacillary dysentery. The pathogen is generally spread through food or water contaminated by feces. There are a large number of reports of diseases caused by Shigella infection all over the world every year, and the threat of this bacteria in my country is also increasing. Therefore, how to quickly, accurately and specifically isolate and identify Shigella from food sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
Inventor 黄岭芳邱国建卢勉飞蔡芷荷吴清平田亮
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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