Loop-mediated isothermal amplification (LAMP) detection method for identifying beef and mutton

Abstract

The invention discloses a loop-mediated isothermal amplification (LAMP) detection method for identifying beef and mutton, falling into the field of molecular genetics. The method comprises searching in GenBank to obtain cattle species-specific conserved sequence designed primers, and performing specific screening on designed primers by using LAMP real-time turbidometer; preparing LAMP reaction solution to construct detection system; taking 1muL extracted genome DNA and 1muL fluorescence detection reagent, adding into the LAMP reaction solution to perform LAMP, and identifying according to color of reaction solution, wherein if reaction solution turns into green, the sample contains beef or mutton. The method has convenient and precise detection, easy preparation of templates and primers, high specificity and sensitivity, naked-eye observation of detection result, and wide application prospect; and can satisfy detection requirements of each detection department.

Description

technical field [0001] The invention relates to using LAMP technology to identify the authenticity of beef and mutton, and belongs to the field of molecular genetics. Background technique [0002] At present, the rapid rise and shortage of domestic beef prices have led to a large number of foreign beef entering China through smuggling and other channels. At the same time, there have also been many discordant phenomena in China. In April 2011, Anhui first exposed the use of beef paste and other additives to make pork mutated. Since the beef incident, similar products have been found in many provinces and cities across the country. For a time, people's concerns about the safety of beef and other foods have become the focus of the whole society. In fact, the pork-to-beef incident facing the beef market is only the tip of the iceberg. There are still a lot of people in the society that use chicken, duck and other low-priced meat and even some dead meat to pass off as beef. Fa...

AI Technical Summary

Problems solved by technology

As early as 2008, Xinhua Daily Telegraph reported that a citizen in Hangzhou bought 10 yuan of beef and spent 2,800 yuan for DNA testing. In 2010, Zhongxin.com reported that a consumer in Ningbo spent 1,800 yuan to identify the authenticity of beef through DNA paternity testing technology. events, indicating that although this method can be identified, it takes a long time and costs a lot

Images