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Bacillus coli O157:H7 antigen rapid detection testing strip

A technology of O157 and Escherichia coli, which is applied to the determination/testing of microorganisms, biochemical equipment and methods, etc., and can solve the problems of complicated operation, high price and low sensitivity

Inactive Publication Date: 2007-12-05
贝瑞特生物技术(郑州)有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not conducive to timely diagnosis and implementation of corresponding control measures
Some other auxiliary diagnostic methods such as agglutination test and immunofluorescence method generally have problems such as low sensitivity and poor specificity, and some require special instruments and complicated operations; foreign imported reagents are expensive

Method used

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  • Bacillus coli O157:H7 antigen rapid detection testing strip

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Experimental program
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Embodiment

[0013] Embodiment: Escherichia coli O157: H7 antigen rapid detection test strip, including PVC substrate 1, the absorbent cotton adhered to the upper part of the substrate (in this embodiment, the upper and lower parts are based on the swimming direction of the specimen sample when the test strip is used) Pulp board 2, glass fiber membrane 3 adhered to the lower part of the base material, nitrocellulose membrane 4 adhered to the middle part of the base material, self-adhesive coating 6 adhered to the middle part of the base material on the glass fiber membrane 3, and the coating A piece of non-woven fabric 5 is clamped and fixed between 6 and glass fiber 3 . The absorbent cotton pulp board 3, the nitrocellulose membrane 4, the matrix 5 and the end surfaces of the glass fiber membrane 6 are in close contact. The colloidal gold-labeled monoclonal antibody against the Escherichia coli O157:H7 antigen was adsorbed on the matrix 5, and the concentration of the colloidal gold antibo...

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PUM

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Abstract

The test strip for fast detecting colibacillus O157:H7 antigen belongs to the field of medical diagnosis article technology. The fast test strip includes one base substrate, one fiber film covering the base substrate, one water absorbing film covering the upper part of the base substrate, monoclonal marking antibody of colibacillus O157:H7 antigen adsorbed onto the fiber film in the lower part of the test strip, one anti-mouse IgG coated quality controlling line on the middle part of the fiber film, and one detecting line coated with monoclonal antibody of colibacillus O157:H7 antigen with excellent pairing property to absorbed marking antibody below the quality controlling line. The marking antibody has marker of colloidal gold, the concentration of colloidal gold antibody is (1.0-3.0)x10<->3 mg / sq cm, and that of the coating antibody is (2.0-8.0)x10<->4 mg / sq cm. The present invention has high detection speed and high detection accuracy.

Description

technical field [0001] The invention belongs to the technical field of medical diagnostic products, in particular to a test strip for rapid detection of Escherichia coli O157:H7. Background technique [0002] Escherichia coli O157:H7 is the main serotype of enterohaemorrhagic Escherichia coli, which can be transmitted through beef and its products, milk and its products, chicken, vegetables, fruits, beverages, water, etc., and can also be transmitted by close contact between people. Escherichia coli O157:H7 can cause hemorrhagic enteritis, the typical clinical symptoms of infected patients are bloody stool, abdominal pain, low fever or no fever, about 10% of patients develop hemolytic uremic syndrome and thrombotic thrombocytopenic purpura, and can Causes damage to the intestines, liver, spleen, kidneys, lymph, brain, respiratory and nervous systems of infected patients. The elderly and children are high-risk groups, prone to hemolytic uremia, and the mortality rate is as h...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
Inventor 刘奇张燕范永丽杨伟东杜录堂杜建平
Owner 贝瑞特生物技术(郑州)有限责任公司
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