Dye method digital quantitative PCR kit as well as application and application method thereof

Abstract

The invention discloses a dye method digital quantitative PCR kit as well as application and an application method thereof. The dye method digital quantitative PCR kit comprises application of circular RNA hsa_circ_0051778 copy number as a detection index in a lung adenocarcinoma and tuberculous pleuritis identification kit according to the circular RNA hsa_circ_0051778 copy number in tuberculouspleuritis being higher than the copy number in the lung adenocarcinoma. The kit comprises a circular RNA hsa_circ_0051778 specific primer, a positive control, a negative control, a special PCR amplification premix solution for dye-method digital PCR as well as sterile deionized water; and the kit is used by virtue of steps of sampling, PCR amplification system preparation, amplification reaction and identification. By adopting the dye method digital quantitative PCR kit, the lung adenocarcinoma and the tuberculous pleuritis can be accurately and rapidly identified. The detection result provesthat the sensitivity of the detection tool can reach about 90 percent or more which is higher than the existing single method used clinically.

Description

technical field [0001] The invention relates to a dye method digital quantitative PCR kit and its application and use method, in particular to a dye method digital quantitative PCR kit based on circular RNA hsa_circ_0051778 as a detection index and its application and use method, belonging to medical detection tools . Background technique [0002] Lung adenocarcinoma and tuberculous pleurisy are both clinically common respiratory diseases, and some clinical manifestations of the two diseases are similar, but their treatment options and prognosis are significantly different. Therefore, accurate diagnosis of these two diseases is of great importance to patients. Prognosis is extremely important. [0003] Pleural effusion refers to the abnormal increase of pleural fluid caused by certain diseases affecting the pleura. Lung adenocarcinoma and tuberculous pleurisy are common causes of pleural effusion. At present, the diagnosis can be made by detecting Mycobacterium tuberculosi...

AI Technical Summary

Problems solved by technology

Lung adenocarcinoma and tuberculous pleurisy are common causes of pleural effusion. At present, the diagnosis can be made by detecting Mycobacterium tuberculosis or tumor cells in pleural effusion, but the diagnosis will be confirmed clinically through medical history, blood test, pleural effusion biochemical test, and erythrocyte sedimentation rate test. , tuberculosis antibody detection, cell chromosome detection, tumor antigen detection, etc. to make differential diagnosis between the two, the identification process is cumbersome, time-consuming, and increases the economic burden of patients, so the positive detection rate in actual clinical work is low

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