Quick test paper for organic phosphorus and carbamic acid ester type pesticide residues
A kind of technology of carbamates, pesticide residues
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[0023] A test paper for rapid detection of organophosphorus and carbamate pesticide residues, comprising the following manufacturing steps:
[0024] (1) Preparation of thermosensitive microcapsules
[0025] According to the ratio of 2g: 100mL, prepare the mixture of agarose and 0.1mol / L, pH = 7.0 phosphate buffer solution, put it into a pressure cooker and heat it to 0.05KPa, keep it for 15-20min, and naturally cool it to 37℃ under normal pressure, then Add 20ml, 13% NaOH solution dropwise, add 1.5ml xylenol sulfate dropwise, carry out the methylation reaction at a constant temperature of 37°C, and add 0.5mol / L CH 3 COOH, adjust the pH to neutral pH=7.0, add a certain amount of 1U / ml cholinesterase, add sterilized paraffin oil at 37°C to make it evenly dispersed, put it in an ice water bath at 0°C to solidify, 5000r / Min centrifuge to remove the oil phase for 3 min, repeat 3 times, after the oil phase is removed, the temperature-sensitive microcapsules are obtained;
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