Quick detecting coliform group and medium for culturing coliforms and preparation method

Abstract

This invention relates to a culture medium for the rapid detection of coliform and coliform groups, which is mixed from distilled water solution containing peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free), the solution of indophenol-beta-D-glucoside and solution of cefsulodin. Peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free) are weighed according to a certain criterion and added to distilled water for heat dissolution. The consequent solution is adjusted to a certain pH value, put into a high pressure sterilizer for sterilization and then added with filtrated and sterilized indophenol-beta-D-glucoside solution and cefsulodin solution after the temperature lowered to 50 deg.Cí‚5 deg.C. This is useful in either qualitative or quantitative detection of coliform and coliform groups in drinking water, environmental water and foods, high efficiency and accurate detection result.

Description

technical field

[0001] The invention relates to sanitation and epidemic prevention detection and environmental water quality monitoring technology, in particular to a culture medium for rapid detection of coliforms and Escherichia coli in drinking water, environmental water or food and a preparation method thereof. Background technique

[0002] In water or food, coliforms and Escherichia coli are used as the index bacteria for samples contaminated by enteropathogenic bacteria, and are one of the important indicators for evaluating water quality and food hygiene quality. Therefore, it can quickly and accurately detect water or Coliforms and Escherichia coli in food are of great significance. At present, there are two standards for the detection of coliforms and E. coli in food in China: one is the national standard, that is, the 9-tube fermentation method, which only stipulates the detection of coliforms. The detection steps are divided into three steps. The first step is th...