Method for detecting helicobacter pylori infection in gastric juice

A technology for detection of Helicobacter pylori and infection, applied in the field of enzyme analysis, can solve problems such as discomfort, easy recurrence, slow course of disease, etc.

Inactive Publication Date: 2015-04-29
TIANJIN BAODI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main clinical manifestations are: epigastric discomfort, dull pain, and sometimes belching, acid...

Method used

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  • Method for detecting helicobacter pylori infection in gastric juice
  • Method for detecting helicobacter pylori infection in gastric juice
  • Method for detecting helicobacter pylori infection in gastric juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] 1. Fabrication of the reaction device

[0008] Three concave holes are formed on the rectangular plastic template, and the concave holes are respectively covered with acetate fiber film. The film in the left control area is not soaked with urea solution, and the film on the right side is soaked with urea solution and has been dried. A filter is placed in the center of the template, and the center of the filter is made of thick sponge fibers and covers the middle hole of the reaction device.

[0009] 2. Reagent configuration

[0010] 1. Phenol reagent: 10g of phenol, 0.05g of sodium nitrosoferricyanide (containing 2 molecules of water), dissolved in 1000ml of deaminated distilled water.

[0011] 2. Alkaline sodium hypochlorite solution: Dissolve 5g of sodium hydroxide in deammonized distilled water, add 8ml of antiformin, add 1000ml of distilled water, and store in a brown bottle.

[0012] 3. Pretreatment solution: phosphate-tween 20 buffer solution containing 30 mg / L ...

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PUM

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Abstract

The invention discloses a reagent for detecting helicobacter pylori infection in gastric juice and a detection method thereof and belongs to the technical field of enzyme analysis. According to the prepared reagent, viscous materials in the gastric juice are decomposed into a soluble liquid by virtue of ambroxol hydrochloride, so that the helicobacter pylori in the gastric juice is dissociated and is added into a filter, the filter is taken down from a reaction device, free helicobacter pylori flows into a reaction hole along with a pretreatment fluid, a phenol reagent and an alkaline sodium hypochlorite solution are added, the helicobacter pylori has a urease strengthening effect, the urea is decomposed to generate dimolecular ammonia and monomolecular carbon dioxide after the helicobacter pylori is reacted with urea on an acetate fiber membrane, the ammonia is reacted with phenol and hypochlorous acid in an alkaline medium so as to generate blue indophenol, and the content of the blue indophenol is in direct proportion to the content of the helicobacter pylori. The detection method disclosed by the invention is simple in pretreatment, rapid, sensitive, accurate and low in price, is suitable for detecting the helicobacter pylori in the gastric juice and can serve as an important check index of detection of the helicobacter pylori in the gastric juice.

Description

technical field [0001] The invention relates to a reagent for detecting Helicobacter pylori infection in gastric juice and a preparation method thereof, belonging to the technical field of enzyme analysis. Background technique [0002] Helicobacter pylori or Helicobacter pylori, English name Helicobacter pylori, referred to as Hp, Gram-negative, microaerophilic bacteria, live in various areas of the stomach and duodenum, it can cause mild chronic inflammation of the gastric mucosa, It may even cause gastric and duodenal ulcers and gastric cancer. It is a unipolar, multi-flagellated, blunt-rounded, spirally curved bacterium with a length of 2.5-4.0 μm and a width of 0.5-1.0 μm. The ambient oxygen requirement is 5-8%, and it cannot grow in the atmosphere or in an absolutely anaerobic environment. It mainly includes: factors that enable Helicobacter pylori to penetrate the mucus layer and settle on the surface of gastric epithelial cells, and toxin factors that damage gastric e...

Claims

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Application Information

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IPC IPC(8): C12Q1/58C12Q1/04C12R1/01
Inventor 李立和王彦平常玉芝
Owner TIANJIN BAODI HOSPITAL
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