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A strain of Lactobacillus plantarum that degrades nitrite and has the ability to inhibit ace

A nitrite, lactobacillus technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problem of not screening out high-efficiency degrading lactic acid strains, etc.

Active Publication Date: 2021-05-07
潍坊益昊生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no lactic acid bacteria strain that can degrade nitrite efficiently and inhibit ACE activity at present.

Method used

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  • A strain of Lactobacillus plantarum that degrades nitrite and has the ability to inhibit ace
  • A strain of Lactobacillus plantarum that degrades nitrite and has the ability to inhibit ace
  • A strain of Lactobacillus plantarum that degrades nitrite and has the ability to inhibit ace

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Isolation screening, identification of embodiment 1 bacterial strain

[0032] 1) Isolation and screening of lactic acid bacteria

[0033] Collect fermented food from many companies, including ham, flavored fermented milk, yogurt, kimchi, etc. Select batches with stable quality, good color, and long storage time, and then screen the batch of samples for strains. Take 25g of the solid sample and 25mL of the liquid sample, add it to 225mL of sterile diluent, and prepare a 1:10 homogeneous sample solution. Continue to perform 10-fold gradient dilution, select 2 to 3 samples of appropriate dilution and uniformly spread MRS medium containing calcium carbonate, and after cultivation, colonies with calcium-dissolving circles are selected as the primary screening lactic acid bacteria strains.

[0034] 2) Screening of bacterial strains capable of degrading nitrite

[0035] Seven bacterial strains of a, b, c, d, e, f and g were activated in MRS medium ( figure 1 ). After cult...

Embodiment 2

[0044] The characteristic of embodiment 2 bacterial strains

[0045] 1) Determination of growth curve

[0046] After the strain is activated, take the seeds in the stable period and insert them into the MRS at 1% inoculation amount and place them for cultivation at 35°C. Take a certain amount of bacterial solution at regular intervals, dilute to an appropriate multiple, measure the OD600 absorbance value, record the results, and draw the growth curve. The growth curve measurement results show that Lactobacillus plantarum belongs to the incubation period of strain growth in 0-6 hours, belongs to the logarithmic phase of strain growth in 6-18 hours, and belongs to the stable period of strain growth after 18 hours. It can be seen that the strain grows faster in the MRS liquid medium ( Figure 4 ).

[0047] 2) The acid production ability of the strain

[0048]A single colony was picked and cultured in MRS liquid medium at 35°C, and the pH of the supernatant was measured after 2...

Embodiment 3

[0051] The influence of embodiment 3 environmental factors on degrading nitrite

[0052] 1) Effect of temperature on nitrite degradation rate

[0053] After activating the strain, take 18-hour-old seeds and inoculate them with 0.12g / L NaNO at 1% inoculum 2 The MRS was placed in an incubator at 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, and 42°C for 18 hours, and then a certain amount of bacterial solution was taken, diluted to an appropriate multiple, and the absorbance value was measured. and record the results. The research results show that the degradation of nitrite by Lactobacillus plantarum has a certain temperature requirement. It basically does not degrade at 10°C and 15°C. The degradation rate of nitrite increases rapidly at 15°C-30°C, and the degradation rate is the highest at 35°C, which can reach 93%. . The ability to degrade nitrite weakens after 35°C, probably because the optimum growth temperature of the bacteria is 35°C, and the concentration of bacteria at 35°C is...

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Abstract

The invention provides a Lactobacillus plantarum strain with nitrite degrading activity and ACE inhibitory activity and application thereof, which has degrading effect on nitrite and can be widely used in fermented food and fermented feed. The preservation number of Lactobacillus sp. FJS 002 provided by the present invention is CGMCC No 18378. The plant lactobacillus provided by the invention is applied in the field of food processing or feed processing. The present invention obtains excellent bacterial strains with efficient degradative effect on nitrite and inhibitory effect on ACE by screening, and can provide theoretical basis and scientific knowledge for solving the problem of nitrite residue in food, especially fermented food, and the high blood pressure that plagues human health. means.

Description

technical field [0001] The invention belongs to the technical field of food microorganism screening, and in particular relates to a strain of Lactobacillus plantarum with nitrite degrading activity and ACE inhibitory activity. Background technique [0002] The problem of nitrite is a hot issue in today's food industry. Nitrite exists in soil, water, animals and plants. The excessive use of nitrogen fertilizer in agriculture and the imbalance of N, P and K fertilizer proportions cause a large amount of nitrite in vegetables. One of the causes of salt, the discharge of waste water in industry, is also one of the reasons that cause the nitrite content in processed agricultural products to exceed the standard. Nitrite is harmless to plants itself, but it is extremely harmful to human and animal health. Ingesting a large amount of nitrite can easily poison the body and cause cancer. Human beings belong to the top of the food chain and will be enriched through the food chain. Ni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20A61P9/12C12R1/25
CPCA23L5/28A61P9/12C12N1/20C12N1/205C12R2001/25
Inventor 朱英莲杨庆利
Owner 潍坊益昊生物技术有限公司
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