A kind of construction method of Saccharomyces cerevisiae isobutanol high-yielding strain

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae, which is applied in the field of DNA recombination technology to prepare microorganisms, and can solve problems such as imbalance and restricting the yield of isobutanol.
CN103789341BActive Publication Date: 2016-05-18HEBEI UNIV OF TECH
3 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEBEI UNIV OF TECH
Publication Date
2016-05-18

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a construction method of a saccharomyces cerevisiae strain with a high yield of isobutanol and relates to preparation of microorganism by a recombinant DNA (Deoxyribonucleic Acid) technology. According to the method, the saccharomyces cerevisiae strain with the high yield of isobutanol is constructed by reforming saccharomyces cerevisiae cells by using molecular biology and the gene recombination technology. The method comprises the following steps: S1, constructing plasmids of over-expressing ILV2 (Induced Leukemia Virus), ILV3, ILV5 and ZWF1 genes of saccharomyces cerevisiae; and S2, constructing a saccharomyces cerevisiae strain HBGDAL-103 with the high yield of isobutanol. The method disclosed by the invention overcomes the deficiency that further improvement of the yield of isobutanol is restricted by factors such as imbalance of a coenzyme NADPH / NADP<+> (Reduced Form of Nicotinamide-Adenine Dinucleotide Phosphate) / (Nicotinamide-Adenine Dinucleotide Phosphate<+>) from pyruvic acid to 2-one isovaleric acid due to over-expressed ILV2, ILV3 and ILV5 in the saccharomyces cerevisiae cells of yielding isobutanol in the prior art.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The technical scheme of the invention relates to the preparation of microorganisms by DNA recombination technology, in particular to a method for constructing a high-yield isobutanol strain of Saccharomyces cerevisiae. Background technique

[0002] Isobutanol has low volatility, corrosiveness, high octane number and energy density, and is easy to store and transport. It is a new type of biofuel with huge market potential. Saccharomyces cerevisiae can be used as the dominant host strain for isobutanol production due to its good tolerance to isobutanol, the advantage of utilizing cellulose hydrolyzate and its own advantage of synthesizing isobutanol gene.

[0003] In the prior art, due to overexpression of ILV2, ILV3 and ILV5 genes in isobutanol-producing Saccharomyces cerevisiae cells, the coenzymes NADPH / NADP from pyruvate to 2-ketoisovalerate + The imbalance restricts the further improvement of isobutanol production, these defects are in Image 6 dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More