Method for improving competitive nodulation capacity of nodule bacteria USDA110

A rhizobia and nodulation technology, applied in the field of genetic engineering, can solve the problems of inoculated bacteria failing to achieve the expected effect, weak nitrogen fixation ability, and reducing the nodule occupancy rate of inoculated rhizobia, so as to improve the competitive nodulation ability and nitrogen fixation ability. , the effect of good symbiotic nitrogen fixation and competitive nodulation ability
CN110452864AActive Publication Date: 2019-11-15华创佳农生物科技(武汉)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
华创佳农生物科技(武汉)有限公司
Publication Date
2019-11-15

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Abstract

The invention discloses a method for improving a competitive nodulation capacity of nodule bacteria USDA110. A dgoK1 singly inserted mutant strain USDA110 (dgoKmut) is constructed through pK19mob, under the soil potting condition, compared with a plant inoculated with USDA110, the fresh weight, nodule number, nodule weight and enzyme activity of a plant inoculated with USDA110 (dgoKmut) are all significantly increased; compared with indigenous nodule bacteria, the competitive nodulation capacity of the USDA110 (dgoKmut) is improved by 24.5%, and can reach 31.8%. Therefore, the USDA110 (dgoKmut) has good symbiotic nitrogen fixation and competitive nodulation capacities, and can be used as a promising strain in field experiments.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for improving the nodulation competition ability of rhizobia USDA110. Background technique

[0002] China accounts for 8%-10% of the world's arable land, but the use of chemical fertilizers accounts for 35% of the world. Excessive use of chemical fertilizers not only increases the economic burden of farmers, but also seriously damages the natural environment. The fertilizer utilization rate of my country's main food crops is only 20%-35%, while that of developed countries is 40%-50%. At the same time, my country releases more than 20 million tons of nitrogen fertilizer and more than 10 million tons of phosphate fertilizer to the natural environment every year. Excessive use of nitrogen fertilizers has caused surface and groundwater pollution and soil acidification. We must pay attention to environmental pollution caused by excessive use of chemi...

Claims

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