Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea
A technology of Botrytis cinerea and Blight blight, which is applied in the field of microorganisms, can solve problems such as insecurity, and achieve a strong inhibitory effect
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Embodiment 1
[0020] Example 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)
[0021] The present invention obtained amylase chromogenic streptomyces ( Streptomyces diastatochromogenes ) D, save.
[0022] Amylase Streptomyces chromogenes ( S. diastatochromogenes ) Determination of antibacterial activity of D: Take 1 mL of the fermentation supernatant in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50°C. After cooling, put 1 diameter Cucumber solanum blight or tomato Botrytis cinerea with a thickness of 4 mm was tested. The cake was connected to the center of the petri dish (the diameter of the petri dish was 9 cm), and cultured in an incubator at 28°C for 36 h. The treatment was repeated 3 times as a control; the colony diameter was determined by the cross method, and the inhibition rate was calculated by the following formula:
[0023]
[0024] The results showed that the inhibition rate of amylase Streptomyces chromogenes D metaboli...
Embodiment 2
[0025] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolites)
[0026] Follow these steps:
[0027] (1) The slant surface activation culture of the strain test tube: the slant medium is Gaoshi No. 1 medium, and its composition and content are: K 2 HPO 4 0.5 g, KNO 3 1 g, MgSO 4 0.5 g, 20 g soluble starch, 0.5 g NaCl, FeSO 4 0.01 g, 20 g of agar, after dissolving, make up water to 1L; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15× 150 mm), put it in an incubator at 28°C±1°C, activate and culture for 96 h, until the inclined surface of the test tube is covered with spores;
[0028] (2) Fermentation culture: The fermentation medium contains 20 g of soluble starch per 1000 mL, 40 g of soybean flour, NH 4 Cl 3 g, CaCO 3 5 g, MgSO 4 2 g, the balance is water; 60 mL of fermentation broth per 300 m...
Embodiment 3
[0030] Embodiment 3: (tetramycin P is to the inhibition of cucumber solanum blight or tomato Botrytis cinerea)
[0031] (1) Prepare tetramycin P solutions of various concentrations;
[0032] (2) Determination of antibacterial activity: Take 1 mL of tetramycin P solution of each concentration in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50°C, and place on each medium plane after cooling. Put a 4 mm diameter bacterial cake of cucumber solanum blight or tomato cinerea cinerea, connect the bacterial cake to the center of the petri dish (the diameter of the petri dish is 9 cm), put it in an incubator for 36 h at 28°C, and use conventional chemical The medicament and sterile water treatment were used as controls, repeated 3 times; the colony diameter was measured by the cross method, and the inhibition rate was calculated by the following formula:
[0033]
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