Kit for detection or assisted detection of tuberculous pleurisy

Abstract

The invention discloses a kit for detection or assisted detection of tuberculous pleurisy. The kit comprises a device for detecting the expression amount of an immune factor, wherein the immune factor comprises (1) IL-6, IL-13, G-CSF, CCL1, CCL21, IP-10 (CXCL10), CCL8 (MCP-2), CXCL9 (MIG) and CXCL12 (SDF-1); or (2) at least one of IL-6, IL-13, G-CSF, CCL1, CCL21, IP-10 (CXCL10), CCL8 (MCP-2), CXCL9 (MIG) and CXCL12 (SDF-1). Experiments prove that a method for specifically identifying tuberculous pleurisy by taking hydrothorax as a detection object, utilizing a group of immunologic molecular marker, and adopting a multiple-factor microsphere detection technique is developed, and the purposes of improving the disease diagnosis accuracy rate, scientifically judging the state of illness and the like are realized by a multiple-factor combination manner.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting or assisting in detecting tuberculous pleurisy. Background technique [0002] Tuberculosis is an infectious disease that seriously endangers people's health and the national economy. Nearly one-third of the world's population is infected with Mycobacterium tuberculosis. Tuberculous pleurisy is an important cause of pleural effusion in high-incidence areas of tuberculosis. The most reliable diagnosis of tuberculous pleurisy is the presence of Mycobacterium tuberculosis or the presence of granulomas of pleural tissue by biopsy. However, the number of Mycobacterium tuberculosis in pleural effusion is usually very small, and the detection sensitivity of acid-fast staining is extremely low, and tissue sectioning is an invasive diagnostic method, so there are limitations in clinical operations. Therefore, some diagnostic methods using biomolecular markers have emerged ...

AI Technical Summary

Problems solved by technology

Therefore, some diagnostic methods using biomolecular markers have emerged one after another, such as adenosine deaminase (adenosine deaminase, ADA) and IFN-γ. Diagnostic of some value, but less than ideal disease specificity as both factors are also elevated in some other diseases

At the same time, studies have also shown that the high IFN-γ background affects the accuracy of the Mycobacterium tuberculosis-specific IFN-γ release assay (IGRA) of pleural effusion cells, and in some patients, the number of pleural effusion cells obtained is relatively small, which cannot meet the requirements. Test requirements

In addition, tuberculous pleurisy hydrothorax is often misdiagnosed as cancerous hydrops. Therefore, a new simple and easy diagnostic method for tuberculous pleurisy is urgently needed to be developed

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