Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland
A lung adenocarcinoma cell line and human lung adenocarcinoma technology, applied in the field of Chinese lung adenocarcinoma cell lines, can solve the problems of delayed research progress in the diagnosis and treatment of lung cancer bone metastases, limited collection of clinical specimens, etc., and achieve uniformity and repeatability. Good, deepening understanding, fast growth effect
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Embodiment 1
[0036] The pericardial effusion obtained from the first-diagnosed lung adenocarcinoma patient (57-year-old female) was used for primary cell culture. Collect 50 ml of fresh pericardial effusion, aspirate 8 ml of pleural effusion per cell culture bottle, add 2 ml of RPMI1640 medium without bovine serum and double antibody, shake well, and put it in a German culture room with a culture temperature of 37 °C and a CO2 concentration of 5%. Cultured in a Heraeus BB16 carbon dioxide incubator. Four days later, when observed under a light microscope, it was found that about 20% of the bottom of the cell culture flask was covered with cells. The cells grow adherently; most of the adherent cells are circular. In the early stage of culture, the cells grew slowly; the cell morphology was stable, and when it was passed to the 8th passage, the cell morphology and growth rate became slightly faster. The properties remain unchanged after cryopreservation and resuscitation. The growth of CP...
Embodiment 2
[0039] Human lung adenocarcinoma cell (CPA-Yang4) 70×10 4 , injected into the blood system of immunodeficient mice, weighed every other day after two weeks, and performed weekly radionuclide bone scintigraphy from the third week.
[0040] The medium is RPMI1640 or DMEM medium containing 10% fetal bovine serum, pH=7, the culture temperature is 37°C, 5% CO 2 .
Embodiment 3
[0042] Compared with the SPC-A-1 Chinese lung adenocarcinoma established in the early 1980s, CPA-Yang4 has a shorter and faster first metastasis time. SPC-A-1's first bone metastases were 2 / 19 and were not found until 120 days. CPA-Yang4 was inoculated into the left ventricle in the same way, and severe weight loss occurred in about 3 weeks, and he was dying.
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