Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland

A lung adenocarcinoma cell line and human lung adenocarcinoma technology, applied in the field of Chinese lung adenocarcinoma cell lines, can solve the problems of delayed research progress in the diagnosis and treatment of lung cancer bone metastases, limited collection of clinical specimens, etc., and achieve uniformity and repeatability. Good, deepening understanding, fast growth effect

Inactive Publication Date: 2011-01-26
SHANGHAI CHEST HOSPITAL
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Problems solved by technology

[0004] Since the occurrence of lung cancer bone metastases is mostly seen in patients with advanced surgical contraindications, the cli

Method used

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  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland
  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland
  • Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The pericardial effusion obtained from the first-diagnosed lung adenocarcinoma patient (57-year-old female) was used for primary cell culture. Collect 50 ml of fresh pericardial effusion, aspirate 8 ml of pleural effusion per cell culture bottle, add 2 ml of RPMI1640 medium without bovine serum and double antibody, shake well, and put it in a German culture room with a culture temperature of 37 °C and a CO2 concentration of 5%. Cultured in a Heraeus BB16 carbon dioxide incubator. Four days later, when observed under a light microscope, it was found that about 20% of the bottom of the cell culture flask was covered with cells. The cells grow adherently; most of the adherent cells are circular. In the early stage of culture, the cells grew slowly; the cell morphology was stable, and when it was passed to the 8th passage, the cell morphology and growth rate became slightly faster. The properties remain unchanged after cryopreservation and resuscitation. The growth of CP...

Embodiment 2

[0039] Human lung adenocarcinoma cell (CPA-Yang4) 70×10 4 , injected into the blood system of immunodeficient mice, weighed every other day after two weeks, and performed weekly radionuclide bone scintigraphy from the third week.

[0040] The medium is RPMI1640 or DMEM medium containing 10% fetal bovine serum, pH=7, the culture temperature is 37°C, 5% CO 2 .

Embodiment 3

[0042] Compared with the SPC-A-1 Chinese lung adenocarcinoma established in the early 1980s, CPA-Yang4 has a shorter and faster first metastasis time. SPC-A-1's first bone metastases were 2 / 19 and were not found until 120 days. CPA-Yang4 was inoculated into the left ventricle in the same way, and severe weight loss occurred in about 3 weeks, and he was dying.

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Abstract

The invention belongs to the filed of microorganism animal cell lines and provides a Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland. According to the invention, hydrothorax of a lung adenocarcinoma first-diagnosis patient is used as primary cells for culturing; the human lung adenocarcinoma cell has the preservation number of CGMCC No.3138 and classified name as human lung adenocarcinoma cell line CPA-Yang4, with the tumorigenesis rate up to 50-100 percent; and the human lung adenocarcinoma cell can grow quickly and metabolize vigorously and has the biological characteristics of mainly transferring the bone, transferring the lever and the salivary gland and other organs as well as classic pathological characteristics of transferring tumor CPA-Yang4 bone and salivary gland. The invention can be used for providing reference data to early diagnosis for transferring the human lung adenocarcinoma bone and further analyzing lung cancer transferred relevant functional genomes and transferred relevant genes and can provide an effective molecule detection method for early diagnosis and curative effect assessment for bone transfer.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, in particular to the use of a Chinese lung adenocarcinoma cell line with high potential for bone, liver and submandibular gland metastasis. Background technique [0002] Lung cancer is one of the malignant diseases that seriously threaten human survival and development, and has become the number one killer of tumor diseases. In 2006, about 174,470 new cases of lung cancer were diagnosed in the United States, accounting for about 13% (men) and 12% (women) of all tumors, and the incidence was second only to skin cancer; about 162,460 people died of lung cancer, accounting for about 10% of all tumors. 31% (men) and 26% (women), the highest mortality rate [1～2] . There are more than 50,000 new cases of lung cancer in Japan every year, and the mortality rate is nearly 90% [3] . A recent survey on the incidence of malignant tumors in Shanghai shows that lung cancer (21....

Claims

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Application Information

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IPC IPC(8): C12N5/08C12Q1/68C12Q1/18C12R1/91
Inventor 杨顺芳
Owner SHANGHAI CHEST HOSPITAL
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