Semi-automatic smearing method of hydrothorax and ascite liquid-base cytology

Abstract

The invention discloses a semi-automatic method for producing pleural and ascites liquid-based cytology. After the sample is collected, it is placed in a preservation solution bottle, and the preservation solution bottle is placed on a vibrator, and then passed through a centrifuge tube with a sharp bottom in a centrifuge. Add the cell dispersion liquid into the sampler, absorb and discard the supernatant in the test tube, remove the remaining liquid, and place the centrifuged sample on the sample delivery device of the film maker, and each sample passes through the spectrometer in turn, and the sample is aspirated The sample is placed on the corresponding slide in the transfer module, and the dried slide can be used for Papanicolaou staining or HE staining for cytological diagnosis, and Feulgen special staining for DNA quantitative analysis. The present invention is mainly used to improve and solve the problems existing in the non-cervical cell extraction, including the small number of cells, the overlapping of cells, the long production time, the background impurities of the produced films, and the low degree of automation. The quality of slides is improved, the number and distribution of cells on slides are more uniform, easy to use, and has the value of popularization and application.

Description

technical field [0001] The invention relates to a medical testing method, in particular to a semi-automatic method for producing pleural and ascites liquid-based cytology. Background technique [0002] Liquid-based cytology has been developed in China for more than 20 years and is widely used in cervical cancer screening and exfoliative cytology examination of other malignant tumors. Liquid-based cytology detection technology is relatively mature at present, and the products are also emerging in an endless stream. From the method of film production, it is mainly divided into two types: membrane type and sedimentation type. Here, centrifugal film production is also classified as sedimentation method. Many brands have been derived, mainly TCT (cervical liquid-based cytology test) which entered the Chinese market in the 1990s. In recent years, BD company-based LCT (super cypress) film production technology has appeared. Generally speaking, the current production technology for...

AI Technical Summary

Problems solved by technology

Generally speaking, the current production technology for cervical exfoliated cell detection has basically been perfected, and subsequent further research is mainly on how to make it more automated and form an assembly line. However, there are still certain limitations for non-cervical malignant tumor cytology detection. Insufficient, mainly reflected in the number of cells after sampling, as well as the background color of the thin slices and the diversity and complexity of impurities, etc., which affect the judgment of pathologists, increasing the burden on doctors and diagnosis time