Hydrothorax and ascite malignant cell embedding method

Abstract

The invention provides a hydrothorax and ascite malignant cell embedding method. The method comprises the following steps: putting hydrothorax and ascite in a plurality of 50-ml centrifugal tubes respectively, adding analytically pure ethyl alcohol into the centrifugal tubes respectively, and uniformly mixing and standing for 2-4h to obtain sediments; sucking up moisture in the sediment, wrapping a specimen by using a fixing mold and putting into a material drawing box; dehydrating the obtained specimen with the ethyl alcohol, and transparentizing the dehydrated specimen by adopting a transparent agent-xylene; putting the transparentized specimen in a 70-DEG C constant-temperature box for wax dipping, wherein an embedding agent is paraffin wax; and embedding the specimen after wax dipping with paraffin in an embedding workstation, pouring the specimen and the paraffin into an embedding box, putting the embedding box into cold water to rapidly solidify the paraffin after the paraffin on the surface of the embedding box is solidified, taking the paraffin block out of the embedding box and storing. The method can greatly increase the positive rate of cast-off cells, and can be used in further diagnosis of molecules, genes, immunohistochemistry and the like.

Description

technical field [0001] The invention belongs to the field of cell biology and relates to a method for embedding malignant cells in pleural effusion. Background technique [0002] The gold standard for diagnosing malignant pleural effusions is to find cancer cells in the pleural fluid. [0003] Diagnosis of exfoliated cells in pleural effusion has been widely used clinically due to its advantages of quickness, simplicity, and less pain for patients, and has become one of the important means of diagnosing diseases, especially playing a key role in the identification of benign and malignant diseases, which can be used for clinical Provide very valuable, so pleural and ascites exfoliated cytology has become an important means of early screening of malignant tumors. [0004] However, the positive rate of diagnosis of pleural effusion is often very low. One of the reasons is the choice of detection method. At present, the exfoliative cytology examination method commonly used in...

AI Technical Summary

Problems solved by technology

However, due to the limitation of the number of specimens and smears, further follow-up molecular and genetic diagnosis cannot be performed.

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