Hydrothorax and ascite malignant cell embedding method

A malignant cell, thoracic and abdominal technology, applied in the field of cell biology, can solve problems such as inability to diagnose molecular and genetic, and achieve the effect of overcoming molecular diagnosis and genetic testing, improving the positive detection rate and improving the quality of slices

Inactive Publication Date: 2014-10-29
北京海思特医学检验实验室有限公司
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Problems solved by technology

However, due to the limitation of the number of specimens and smears, further follow-up molecular and genetic diagnosis cannot be performed.

Method used

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  • Hydrothorax and ascite malignant cell embedding method

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Embodiment Construction

[0017] The specific implementation of the present invention will be further described below in conjunction with the examples, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention.

[0018] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0019] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0020] Such as figure 1 As shown, in the bloody background, the erythrocytes are lysed and broken, the tumor cells are dense and evenly distributed, the tumor cell membrane structure is clear, the nuclear chromatin is basophilic, coarse-grained, the nucleoli are clear, and pathological mitotic figures can be seen. The film can be directly diagnosed as a malignant tumor, b...

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Abstract

The invention provides a hydrothorax and ascite malignant cell embedding method. The method comprises the following steps: putting hydrothorax and ascite in a plurality of 50-ml centrifugal tubes respectively, adding analytically pure ethyl alcohol into the centrifugal tubes respectively, and uniformly mixing and standing for 2-4h to obtain sediments; sucking up moisture in the sediment, wrapping a specimen by using a fixing mold and putting into a material drawing box; dehydrating the obtained specimen with the ethyl alcohol, and transparentizing the dehydrated specimen by adopting a transparent agent-xylene; putting the transparentized specimen in a 70-DEG C constant-temperature box for wax dipping, wherein an embedding agent is paraffin wax; and embedding the specimen after wax dipping with paraffin in an embedding workstation, pouring the specimen and the paraffin into an embedding box, putting the embedding box into cold water to rapidly solidify the paraffin after the paraffin on the surface of the embedding box is solidified, taking the paraffin block out of the embedding box and storing. The method can greatly increase the positive rate of cast-off cells, and can be used in further diagnosis of molecules, genes, immunohistochemistry and the like.

Description

technical field [0001] The invention belongs to the field of cell biology and relates to a method for embedding malignant cells in pleural effusion. Background technique [0002] The gold standard for diagnosing malignant pleural effusions is to find cancer cells in the pleural fluid. [0003] Diagnosis of exfoliated cells in pleural effusion has been widely used clinically due to its advantages of quickness, simplicity, and less pain for patients, and has become one of the important means of diagnosing diseases, especially playing a key role in the identification of benign and malignant diseases, which can be used for clinical Provide very valuable, so pleural and ascites exfoliated cytology has become an important means of early screening of malignant tumors. [0004] However, the positive rate of diagnosis of pleural effusion is often very low. One of the reasons is the choice of detection method. At present, the exfoliative cytology examination method commonly used in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36
Inventor 刘建勇李微张益清王绪华梁超陈忠方国伟黄士昂
Owner 北京海思特医学检验实验室有限公司
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