Quantitative detection kit for human chorionic gonadotropin and application of quantitative detection kit

Abstract

The invention discloses a quantitative detection kit for human chorionic gonadotropin. The quantitative detection kit comprises a reagent R1, a reagent R2 and a calibration material, wherein the reagent R1 comprises a Tris buffer solution, a beta-human chorionic gonadotropin monoclonal antibody and coarse nano latex particles; the reagent R2 comprises a Tris buffer solution, a beta-human chorionic gonadotropin monoclonal antibody, and thin nano latex particles; the calibration material is a beta-human chorionic gonadotropin calibration solution. According to the quantitative detection kit, the concentration of the human chorionic gonadotropin in serum is quantitatively determined by adopting a latex enhanced immune nephelometry; measurement results are accurate; the detection process is convenient and quick; determination results are stable; by adopting a dual-nano-latex technology, the determination linear range is wide and can reach 100,000IU / L, while the determination linear range of existing other methods does not exceed 20,000IU / L; moreover, the quantitative detection kit is suitable for existing various fully automatic biochemical analyzers, low in detection cost, and suitable for developing project determination in batches clinically.

Description

technical field

[0001] The invention relates to the field of medical testing, in particular to a quantitative detection kit for human chorionic gonadotropin and its application. Background technique

[0002] Human chorionic gonadotropin is a glycoprotein secreted by the trophoblast cells of the placenta, which is composed of α- and β-dimer glycoproteins. Among them, the α-subunit is shared with the anterior pituitary hormone, and the β-subunit is specific to human chorionic gonadotropin, which is often used clinically for the diagnosis of early pregnancy. Commonly used early pregnancy test strips detect pregnancy by qualitatively detecting human chorionic gonadotropin in urine. However, in the judgment of abnormal pregnancy and placental function, the diagnosis and condition monitoring of trophoblastic tumors, and the auxiliary diagnosis and condition monitoring of some other tumors, it is necessary to quantitatively detect the concentration of human chorionic gonadotropin ...

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